Disease resistance breeding of<i>Phalaenopsis</i>spp. for tropical environment and molecular marker development for plant selection

Sukma, Dewi; Elina, Juanita; Giyanto, _; Sudarsono, Sudarsono

doi:10.17660/actahortic.2017.1167.36

Cited by 14 publications

(22 citation statements)

References 10 publications

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“…The activities include breeding for new varieties and developing disease resistant ones (Fatimah & Sukma 2011;Elina 2015;Sukma et al 2015;Sukma & Elina 2017;Handini et al 2016;Haristianita et al 2017).…”

mentioning

confidence: 99%

Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisation

Sudarsono¹,

Elina²,

Giyanto³

et al. 2018

Plant Prot. Sci.

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Sudarsono S., Elina J., Giyanto, Sukma D. (2018): Pathogen causing Phalaenopsis soft rot disease -16S rDNA and virulence characterisation. Plant Protect. Sci., 54: 1-8.The pathogen causing Phalaenopsis soft rot disease and developed detached leaf inoculation methods were identified. Based on its 16S rDNA sequences, the pathogen causing soft rot disease in Phalaenopsis was Erwinia chrysanthemi/ dickeya chrysanthemi. Both virulent and avirulent strains were revealed. The detached leaf inoculation assay for E. chrysanthemi/d. chrysanthemi resistance evaluation included wounding and inoculating the detached leaf with 10 8 CFU/ml of bacteria. Soft rot disease symptoms in the inoculated detached leaf were measurable at 20 h after inoculation. The detached leaf assay was applicable for evaluating Phalaenopsis germplasm and progeny resistance in Phalaenopsis breeding programs.

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mentioning

confidence: 99%

Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisation

Sudarsono¹,

Elina²,

Giyanto³

et al. 2018

Plant Prot. Sci.

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“…The total genomic DNA was isolated from a fully open young leaf of the ten orchid accessions, following the basic CTAB protocols for DNA isolation, routinely used for extracting DNA from the fresh young leaf of Phalaenopsis orchids (Handini 2014;Elina et al 2017;Sudarsono et al 2017;Sukma et al 2017;Raynalta et al 2018;Humaira et al 2019). Fresh young leaf tissues (~0.1 g) were ground to powder and transferred to pre-warmed extraction buffer (2% w/v CTAB, 1.4 M NaCl, 20 mM EDTA, 100 mM Tris-HCL, pH 8.0, and 2% v/v β-mercaptoethanol).…”

Section: Total Genomic Dna Isolationmentioning

confidence: 99%

Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids

2020

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Abstract. Sukma D, Handini AS, Sudarsono. 2020. Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids. Biodiversitas 21: 5054-5064. Chalcone synthase (CHS) is a key enzyme in flavonoid biosynthesis. The research aims to isolate and characterize the CHS gene nucleotide sequence diversity of nine Phalaenopsis and one Doritaenopsis genotypes. Genomic DNA was isolated from young leaves and used to PCR amplify the gene using CHS specific degenerate primers. Results of PCR amplification yielded DNA fragments of 700 bp. Upon sequencing and nucleotide sequence analysis, we found that the genomic fragments were partial CHS gene from Phalaenopsis and Doritaenopsis genotypes and deposited the sequences in the NCBI Genebank Database accession numbers of KR184089-KR184098. More analysis of the sequences confirmed they shared ranged from 81-99% sequence identities to known CHS genes from Phalaenopsis deposited in the NCBI Database. They also shared 84-100% amino acid residues identity to CHS polypeptides. Multiple sequence alignment (MSA) and phylogenetic analysis further revealed the determined CHS nucleotide sequences from Phalaenopsis and Doritaenopsis genotypes were closely related to CHS genes from other orchid species.

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“…Tersedianya informasi tentang gen Pto asal Phalaenopsis dan keragaman molekulernya dapat digunakan untuk identifikasi ketahanan Phalaenopsis terhadap infeksi D. dadantii, patogen penyebab busuk lunak pada Phalaenopsis di Indonesia Sukma et al, 2017 …”

Section: Pendahuluanunclassified

Isolasi dan Karakterisasi Gen Pto Asal 20 Aksesi Anggrek Phalaenopsis

Elina¹,

Sukma²,

Giyanto³

et al. 2017

J Agron Indonesia

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Disease resistance breeding ofPhalaenopsisspp. for tropical environment and molecular marker development for plant selection

Cited by 14 publications

References 10 publications

Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisation

Pathogen causing Phalaenopsis soft rot disease - 16S rDNA and virulence characterisation

Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids

Isolasi dan Karakterisasi Gen Pto Asal 20 Aksesi Anggrek Phalaenopsis

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