Disinfectant resistance mechanisms, cross-resistance, and co-resistance

Chapman, John S.

doi:10.1016/s0964-8305(03)00044-1

Cited by 363 publications

(248 citation statements)

References 44 publications

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“…There is growing concern about bacterial cross-resistance to antibiotics, disinfectants, and/or heavy metals in various environments (52)(53)(54)(55). Until now, there have been no available data on the heavy metal tolerance of B. thermosphacta strains.…”

Section: Discussionmentioning

confidence: 99%

Insight into the Genome of Brochothrix thermosphacta, a Problematic Meat Spoilage Bacterium

Stanborough

Fegan

Powell

et al. 2017

Appl Environ Microbiol

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Brochothrix thermosphacta is a dominant but poorly studied meat spoilage organism. It is a close relative of the foodborne pathogen Listeria monocytogenes, and Brochothrix constitutes the second genus in the Listeriaceae family. Here, the genomes of 12 B. thermosphacta strains were sequenced, assembled into draft genomes, characterized, and compared with the genomes of Brochothrix campestris and L. monocytogenes. Phenotypic properties including biogenic amine production and antibiotic and heavy metal susceptibilities were tested. Comparative genomic analyses revealed a high degree of similarity among the B. thermosphacta strains, with bacteriophage genes constituting a significant proportion of the accessory genome. Genes for the production of the malodorous compounds acetate, acetoin, butanediol, and fatty acids were found, as were stress response regulatory genes, which likely play important roles in the spoilage process. Amino acid decarboxylases were not identified in the genomes, and phenotypic testing confirmed their absence. Orthologs of Listeria virulence proteins involved in virulence regulation, intracellular survival, and surface protein anchoring were found; however, key virulence genes were absent. Analysis of antibiotic susceptibility showed that strains were sensitive to the four tested antibiotics, except for one tetracycline-resistant isolate with plasmid-mediated tetracycline resistance genes. Strains tolerated higher levels of copper and cobalt than of cadmium although not at concentrations high enough to categorize the strains as being resistant. This study provides insight into the Brochothrix genome, links previous phenotypic data and data provided here to the gene inventory, and identifies genes that may contribute to the persistence of this organism in the food chain.IMPORTANCE Despite increasing knowledge and advances in food preservation techniques, microbial spoilage of foods causes substantial losses, with negative social and economic consequences. To better control the contamination and microbial spoilage of foods, fundamental knowledge of the biology of key spoilage bacteria is crucial. As a common meat spoilage organism, B. thermosphacta contributes substantially to spoilage-associated losses. Nonetheless, this organism and particularly its genome remain largely unstudied. This study contributes to improving our knowledge of the Brochothrix genus. Spoilage-relevant pathways and genes that may play a role in the survival of this organism in a food processing environment were identified, linking previous phenotypic data and data provided here to the gene inventory of Brochothrix and establishing parallels to and differences from the closely related foodborne pathogen L. monocytogenes.KEYWORDS Brochothrix, Listeria monocytogenes, meat spoilage, spoilage B rochothrix thermosphacta is one of the most abundant spoilage organisms of fresh and cured meats, fish, and fish products, due to its tolerance to high-salt and low-pH conditions, its ability to grow at refrigeration tempera...

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Section: Discussionmentioning

confidence: 99%

Insight into the Genome of Brochothrix thermosphacta, a Problematic Meat Spoilage Bacterium

Stanborough

Fegan

Powell

et al. 2017

Appl Environ Microbiol

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“…are Gram positive pathogens associated with foodborne outbreaks. These bacteria have virulence factors, such as, aggregation substances, extracellular surface proteins, and ability to form biofilms (Chapman 2003, Fisher and Phillips 2009, Marinho et al 2013. The E. faecalis was responsible for an outbreak with lenezolid resistance (LRE) in a hospital, affecting 13 people between 2004 and 2005 in Tennessee, USA (Kainer et al 2007).…”

mentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

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The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocinlike substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

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“…There is no clear evidence that there is a link between antibiotic resistance and biocide resistance and studies still continue in this area (3,5,7,20,23). The fact that antibiotic and biocide resistance mechanisms are similar suggests that there is a possible link between them (3).…”

Section: Discussionmentioning

confidence: 99%

An investigation of the bactericidal activity of chlorhexidine digluconateagainst multidrug-resistant hospital isolates

Ekizoğlu¹,

Sağıroğlu²,

Kılıç³

et al. 2016

Turk J Med Sci

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Disinfectant resistance mechanisms, cross-resistance, and co-resistance

Cited by 363 publications

References 44 publications

Insight into the Genome of Brochothrix thermosphacta, a Problematic Meat Spoilage Bacterium

Insight into the Genome of Brochothrix thermosphacta, a Problematic Meat Spoilage Bacterium

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

An investigation of the bactericidal activity of chlorhexidine digluconateagainst multidrug-resistant hospital isolates

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