Disinfection efficacy of green synthesized gold nanoparticles for medical disinfection applications

Abstract

Background In recent times, biosyntheses of metal nanoparticles were used for several life rescue applications. In this study, Dillenia indica leaf aqueous extract was utilized for the synthesis of gold nanoparticles. Objective To test anti-microbial properties of biologically fabricated gold nanoparticles. Methods Gold nanoparticles were efficiently prepared by making use of aqueous leaf extract of Dillenia indica. The excitation of formed AuNPs was confirmed using UV-Vis spectrophotometer. In particular, absorption spectra of AuNPs exhibited a well-defined SPR band centered at around 530 nm. Results The high-resolution Scanning Electron Microscope (SEM) results of the obtained AuNPs confirmed the formation of particles with a size range of 5–50 nm. The ultra-high resolution TEM (UHRTEM) images displayed clear lattice fringes on the particle surfaces. Single crystalline nature of the biosynthesized AuNPs was represented by means of selected-area electron diffraction pattern. Conclusion The antibacterial activity of AuNPs revealed significant activity towards both gram negative and gram positive bacteria signifying their potential disinfection related applications in medicine and biology.

Green Synthesis of Gold Nanoparticles by Aqueous Extract of Zingiber officinale: Characterization and Insight into Antimicrobial, Antioxidant, and In Vitro Cytotoxic Activities

“…This is of the same order of magnitude as the gold nanoparticles synthesized by the mycelium of Mucor plumbeus (i.e., 13-25 nm; average size: 17 ±4 nm, [54]). Consistent with this study, Huang et al [55] reported that the size of AuNPs fabricated by leaf aqueous extract of Dillenia indica was between 5 and 50 nm, whereas the average particle sizes of AuNPs synthesized by aqueous extract of Garcinia mangostana peel and Mentha longifolia leaf were 32.9 ± 5.3 nm and 36.4 nm, respectively [39,56]. The variation in average particle sizes could be attributed to metabolites that varied between the plants used as reducing, capping, and stabilizing agents.…”

Nanoparticles as Anti-Microbial, Anti-Inflammatory, and Remineralizing Agents in Oral Care Cosmetics: A Review of the Current Situation

“…In recent years, research on NP synthesis has mainly focused on green synthesis using bacteria [73,118] and plants [119][120][121][122]. For example, the marine extremophilic bacteria Pseudoalteromonas was used to obtain Au NPs with non-cytotoxic, non-genotoxic and non-oxidative stress generated over a range of concentrations but provoking alterations in DNA methylation and in the expression of DNA methyltransferase genes [118].…”

Antimicrobial Properties of Metal Nanoparticles and Their Oxide Materials and Their Applications in Oral Biology

“…Huang et al tested the antibacterial activity of AuNPs and found that AuNPs have significant antibacterial activity against gram-negative bacteria and gram-positive bacteria, indicating that AuNPs have potential applications in the fields of medicine and biology [21]. Recently, López-Lorente et al found that spherical AuNPs have no antibacterial activity between 0 and 500 mg•L −1 , while flower-shaped AuNPs and star-shaped AuNPs of the same size have antibacterial properties at high concentrations (250~500 mg•L −1 ) [22].…”