Displaying of acetylcholinesterase mutants on surface of yeast for ultra-trace fluorescence detection of organophosphate pesticides with gold nanoclusters

Liang, Bo; Han, Lei

doi:10.1016/j.bios.2019.111825

Cited by 73 publications

(24 citation statements)

References 36 publications

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“…With GSH-AuNCs, the linear ranges were 20–200 ng/mL (R 2 = 0.9991) and 200–500 ng/mL (R 2 = 0.9921), respectively ( Figure 7 B). The limit of detection (LOD) was calculated to be 6.2 ng/mL according to 3S/N [ 56 ]. In the case of GSH/β-CDs-AuNCs, the linear ranges were 5–100 ng/mL (R 2 = 0.9934) and 100–350 ng/mL (R 2 = 0.9954) ( Figure 8 B).…”

Section: Resultsmentioning

confidence: 99%

A Fluorescent Detection for Paraquat Based on β-CDs-Enhanced Fluorescent Gold Nanoclusters

Ren

Mao

et al. 2021

Foods

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In this report, a fluorescent sensing method for paraquat based on gold nanoclusters (AuNCs) is proposed. It was found that paraquat could quench both glutathione-capped AuNCs (GSH-AuNCs) and β-cyclodextrin-modified GSH-AuNCs (GSH/β-CDs-AuNCs). The modification of β-CDs on the surface of GSH-AuNCs obviously enhanced the fluorescence intensity of GSH-AuNCs and improved the sensitivity of paraquat sensing more than 4-fold. This sensibilization was ascribed to the obvious fluorescence intensity enhancement of GSH-AuNCs by β-CDs and the “host–guest” interaction between paraquat and β-CDs. The fluorescence quenching was mainly due to the photoinduced energy transfer (PET) between GSH/β-CDs-AuNCs and paraquat. With the optimized β-CDs modification of the GSH-AuNC surfaces and under buffer conditions, the fluorescent detection for paraquat demonstrated a linear response in the range of 5.0–350 ng/mL with a detection limit of 1.2 ng/mL. The fluorescent method also showed high selectivity toward common pesticides. The interference from metal ions could be easily masked by ethylene diamine tetraacetic acid (EDTA). This method was applied to the measurement of paraquat-spiked water samples and good recoveries (93.6–103.8%) were obtained. The above results indicate that host molecule modification of fluorescent metal NC surfaces has high potential in the development of robust fluorescent sensors.

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Section: Resultsmentioning

confidence: 99%

A Fluorescent Detection for Paraquat Based on β-CDs-Enhanced Fluorescent Gold Nanoclusters

Ren

Mao

et al. 2021

Foods

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“…The fabricated biosensor was also confirmed to be capable of measuring analytes in tap water, seawater, and sewage. Very recently, AChE surface-displaying yeast cells were utilized to fabricate a fluorescence biosensor [ 101 ]. As shown in Figure 4 c, after hydrolysis of ATCl by the surface-displayed AChE, positively charged thiocholine with thiol groups was generated, leading to aggregation of gold nanoclusters (AuNCs) through electrostatic interactions, and the aggregated AuNCs quenched the fluorescence signal.…”

Section: Eukaryotic Yeast Cell Surface Display For Biosensor Applimentioning

confidence: 99%

“…( c ) Paraoxon biosensing using AChE surface displaying yeasts. Reproduced with permission [ 101 ]. Copyright (2018) Elsevier.…”

Section: Figurementioning

confidence: 99%

Surface Display Technology for Biosensor Applications: A Review

Park

2020

Sensors

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Surface display is a recombinant technology that expresses target proteins on cell membranes and can be applied to almost all types of biological entities from viruses to mammalian cells. This technique has been used for various biotechnical and biomedical applications such as drug screening, biocatalysts, library screening, quantitative assays, and biosensors. In this review, the use of surface display technology in biosensor applications is discussed. In detail, phage display, bacterial surface display of Gram-negative and Gram-positive bacteria, and eukaryotic yeast cell surface display systems are presented. The review describes the advantages of surface display systems for biosensor applications and summarizes the applications of surface displays to biosensors.

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“…Detection of OP compounds has been traditionally performed with analytical methods such as gas chromatography (GC) [6], high-performance liquid chromatography (HPLC) [7], capillary electrophoresis [8], surface plasmon resonance [9], fluorimetry [10], and spectroscopic methods [11]. Although these techniques have been standardized, none of these methods are suitable for on-site monitoring and the rapid detection of OPs [12].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric detection of organophosphates with cysteamine capped gold nanoparticle sensors

Shah¹,

Ren²,

Ahmad³

et al. 2020

Preprint

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Nanozyme biosensors have the potential to provide high sensitivity, multiple functionality, and tunable activity. A facile colorimetric biosensor for the detection of organophosphates (OPs) using cysteamine capped gold nanoparticle probes (C-AuNPs) as enzyme mimics is proposed. Parathion ethyl (PE) a class of OPs is a potent insecticide that functions by inhibiting the acetylcholinesterase (AChE) in the nervous system of insects. The inhibition kinetics of AChE using PE enables the development of a PE sensor. C-AuNPs possess the ability to catalyze the oxidization of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) to a blue-colored product without peroxidase. The detection of PE was monitored by the inability of AChE to generate choline. Choline causes the aggregation of C-AuNPs and the aggregated C-AuNPs has decreased ability to catalyze the oxidization of TMB. A calibration was developed in the 40-320 nM range for the quantitative detection of PE. The limit of detection observed was 20 nM and the method had excellent specificity. The proposed sensor provides an excellent platform for on-site monitoring of PE in environmental and food samples with high sensitivity and greater selectivity.

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Displaying of acetylcholinesterase mutants on surface of yeast for ultra-trace fluorescence detection of organophosphate pesticides with gold nanoclusters

Cited by 73 publications

References 36 publications

A Fluorescent Detection for Paraquat Based on β-CDs-Enhanced Fluorescent Gold Nanoclusters

A Fluorescent Detection for Paraquat Based on β-CDs-Enhanced Fluorescent Gold Nanoclusters

Surface Display Technology for Biosensor Applications: A Review

Colorimetric detection of organophosphates with cysteamine capped gold nanoparticle sensors

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