Disposable Electrochemical Enzyme‐Amplified Genosensor forSalmonellaBacteria Detection

Development of disposable low density screen-printed electrode arrays for simultaneous electrochemical measurements of the hybridisation reaction

“…Any contamination effect between adjacent electrodes was observed. This can be considered an interesting progress in respect to previously published papers [11,13] concerning nucleic acid detection using screen-printed transducers. In this sense, the use of the eight WE array will be a further step forward in easy to use tools for screening analysis of nucleic acid.…”

“…In respect to our previously published papers [11][12][13], in which a silver track alone was used as pseudo-reference, it was experimentally observed that the formation of a chloride layer is necessary to stabilise the simultaneous measurements occurring at the four or eight WEs.…”

In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogens

“…Nucleic acid-based detection coupled with PCR has distinct advantages over culture and other standard methods for the detection of microbial pathogens such as specificity, sensitivity, rapidity, accuracy and capacity to detect small amounts of target nucleic acid in a sample. The further amplicon detection can be achieved with electrochemical DNA biosensors (Ye et al, 2003;Kara et al, 2004;Del Giallo et al, 2005), reducing the time of the assay and providing results to genetic specificity. The development of new materials such as magnetic beads has also brought unique opportunities to DNA detection strategies (Haukanes and Kvam, 1993 as specific cells, antibodies, and enzymes -can be selectively bound to the magnetic beads and then separated from its biological matrix by using a magnetic field.…”

Sensitive and Label-Free Detection of DNA by Surface Plasmon Resonance

“…Over the last two decades, a great number of reports have appeared on the advancements in and applications of the SPR technology [30][31][32][33][34][35][36]. SPR has become a powerful tool for characterizing and quantifying biomolecular interactions.…”