Disposable plasmonic plastic SERS sensor

Oo, Swe Zin; Chen, R.Y.; Siitonen, Samuli; Kontturi, Ville; Eustace, David; Tuominen, Jari; Aikio, Sanna; Charlton, Martin D. B.

doi:10.1364/oe.21.018484

Cited by 32 publications

(26 citation statements)

References 27 publications

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“…To further maximize the enhancement factor for 785 nm excitation, we explored the use of these NPs in combination with structured SERS substrates, which had been introduced and described in our previous studies . Such substrates are produced by imprinting of specially profiled patterns on top of a poly(methylmethacrylate) (PMMA) polymer sheet by roll‐to‐roll UV‐nanoimprint lithography, followed by evaporation‐based coating of the patterns by a thin layer of gold, and the integration of the structures into hydrophobic PDMS wells . Figure A shows SERS spectra of dried 100 μM R6G molecules enhanced by Au‐60% and Au‐30% NPs.…”

Section: Resultsmentioning

confidence: 99%

Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification

Kögler

Ryabchikov

Uusitalo

et al. 2018

Journal of Biophotonics

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The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials.

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Section: Resultsmentioning

confidence: 99%

Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification

Kögler

Ryabchikov

Uusitalo

et al. 2018

Journal of Biophotonics

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“…41 The produced polymer webs and die-cut sheets before and after gold deposition are presented in Figure 1. 41 The produced polymer webs and die-cut sheets before and after gold deposition are presented in Figure 1.…”

Section: Sers Substrate Fabricationmentioning

confidence: 99%

“…The conventional way for fabricating structured SERS substrates is to use methods such as spin-coating, dip c oating, chemical vapour deposition, electrochemical synthesis, electron beam lithography and etching. 40,41 The fabricated SERS substrates are coated with a thin layer of gold by evaporation before integration of hydrophobic sample wells. There are also many methods such as liquidliquid interface formation, pulsed laser deposition on microscope slides and reduction of gold chloride III in natural rubber membranes w hich hav e been only tested in la b scale as batc h fabrication.…”

Section: Introductionmentioning

confidence: 99%

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

et al. 2016

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The rapid and accurate detection of food pathogens plays a critical role in the early prevention of foodborne epidemics.Current bacteria identification practises, including colony counting, polymerase chain reaction (PCR) and immunological methods, are time consuming and labour intensive; they are not ideal for achieving the required immediate diagnosis. Different SERS substrates have been studied for the detection of foodborne microbes. The majority of the approaches are either based on costly patterning techniques on silicon or glass wafers or on methods which have not been tested in large scale fabrication. We demonstrate the feasibility of analyte specific sensing using mass-produced, polymer-based low-cost SERS substrate in analysing the chosen model microbe with biological recognition. The use of this novel roll-to-roll fabricated SERS substrate was combined with optimised gold nanoparticles to increase the detection sensitivity.Distinctive SERS spectral bands were recorded for Listeria innocua ATCC 33090 using an in-house build (785 nm) near infra red (NIR) Raman system. Results were compared to both those found in the literature and the results obtained from a commercial time-gated Raman system with a 532 nm wavelength laser excitation. The effect of the SERS enhancer metal and the excitation wavelength on the detected spectra was found to be negligible. The hypothesis that disagreements within the literature regarding bacterial spectral results from conditions present during the detection process has not been supported. The sensitivity of our SERS detection was improved through optimization of the concentration of the sample inside the hydrophobic polydimethylsiloxane (PDMS) wells. Immuno-magnetic separation (IMS) beads were used to assist the accumulation of bacteria into the path of the beam of the excitation laser. With this combination we have detected L i s t e r i a w i t h g o l d e n h a n c e d S E R S i n a l a b e l f r e e m a n n e r f r o m s u c h l o w s a m p l e c o n c e n t r a t i o n s a s 1 0 4 CFU/ml.Figure 10. a) A normalised concentration series for LOD estimation. b) An exponential fit for the normalised concentration series in logarithmic scale for the entire series and a linear fit for the small concentrations. c) Comparison of the 737 cm -1 peak intensity for different concentration series with 5 -10 µl dried IMS bound L. innocua ATCC 33090 samples placed with AuNPs into a 1 -1.5 mm PDMS well on top of SERS substrate. d) Comparison of baseline corrected Raman intensities for three of the concentration series. All figures are a mean of 9 measurement points with mean absolute deviations.

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“…[1][2][3] Examples of regularly structured materials include lithographically prepared substrates, such as Klarite [4] and those prepared on templates [5] , while randomly structured substrates include nanoparticle aggregates [1][2][3] and roughened electrodes [1] . However, the number of materials which show the enhanced Raman scattering is now very large and growing rapidly.…”

Section: Characterization Of Sers-active Materialsmentioning

confidence: 99%

Surface‐Enhanced Raman Spectroscopy as a Probe of the Surface Chemistry of Nanostructured Materials

et al. 2016

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Surface‐enhanced Raman spectroscopy (SERS) is now widely used as a rapid and inexpensive tool for chemical/biochemical analysis. The method can give enormous increases in the intensities of the Raman signals of low‐concentration molecular targets if they are adsorbed on suitable enhancing substrates, which are typically composed of nanostructured Ag or Au. However, the features of SERS that allow it to be used as a chemical sensor also mean that it can be used as a powerful probe of the surface chemistry of any nanostructured material that can provide SERS enhancement. This is important because it is the surface chemistry that controls how these materials interact with their local environment and, in real applications, this interaction can be more important than more commonly measured properties such as morphology or plasmonic absorption. Here, the opportunity that this approach to SERS provides is illustrated with examples where the surface chemistry is both characterized and controlled in order to create functional nanomaterials.

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Disposable plasmonic plastic SERS sensor

Cited by 32 publications

References 27 publications

Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification

Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification

Detection of Listeria innocua on roll-to-roll produced SERS substrates with gold nanoparticles

Surface‐Enhanced Raman Spectroscopy as a Probe of the Surface Chemistry of Nanostructured Materials

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