2018
DOI: 10.1007/s41048-018-0064-0
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Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers

Abstract: The accessibility of genomic DNA, as a key determinant of gene-related processes, is dependent on the packing density and structural dynamics of chromatin fiber. However, due to the highly dynamic and heterogeneous properties of chromatin fiber, it is technically challenging to study these properties of chromatin. Here, we report a strategy for dissecting the dynamics of chromatin fibers based on single-molecule magnetic tweezers. Using magnetic tweezers, we can manipulate the chromatin fiber and trace its ext… Show more

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Cited by 5 publications
(2 citation statements)
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“…Namely, a new era of experimental research has started from the end of the last century. This means that mechanical manipulations on a single molecule, that are stretching, twisting and untwisting, are now possible [155][156][157][158][159][160][161][162][163][164][165]. While the thermodynamics of these manipulations have been extensively studied [166][167][168], there are still ample opportunities for kinetic studies aimed at detecting emergent DNA behaviours as well as DNAprotein interactions, which could be modelled within the frameworks reviewed in this paper.…”
Section: Conclusion and Discussionmentioning
confidence: 99%
“…Namely, a new era of experimental research has started from the end of the last century. This means that mechanical manipulations on a single molecule, that are stretching, twisting and untwisting, are now possible [155][156][157][158][159][160][161][162][163][164][165]. While the thermodynamics of these manipulations have been extensively studied [166][167][168], there are still ample opportunities for kinetic studies aimed at detecting emergent DNA behaviours as well as DNAprotein interactions, which could be modelled within the frameworks reviewed in this paper.…”
Section: Conclusion and Discussionmentioning
confidence: 99%
“…Moreover, SMFS also enables the control of the reaction coordinate for the folding/unfolding of proteins, yielding detailed insights of the complex protein folding free energy landscape . SMFS can be carried out using various techniques, including magnetic tweezers, optical tweezers, and atomic force microscopy (AFM). …”
Section: Introductionmentioning
confidence: 99%