Dissociation-Association Behavior of Arachin between Dimeric and Monomeric Forms

Isolation and Some Properties of Arachin Subunits

“…About three moles of glycine, two moles of isoleucine and one mole of valine were detected as the N-terminal amino acids on the basis of the molecular weight 180,000. 7 ) These results are consistent with that of Y otsuhashi and Shibasaki. Individual arachin subunits were isolated by repeating the preparative isoelectric focusing.…”

“…7) Of the two species of arachin, arachin I monomer (mol. wt., 180,000) was used in this investigation.…”

Application of HPLC, Gel electrophoresis and size exclusion chromatography for separation and characterization of storage proteins of soybean and peanut

“…Retention time and peak ratio of all these 12 subunits are also summarized in Table V. There has been no earlier report with regard to 12 subunits of arachin on the basis of slab gel electrophoresis as well as RP-HPLC, although arachin was reported to contain 12 subunits by osmometry [37,40].…”

“…Since the first evidence for a total of six differently sedimenting proteins based on sedimentation constants determined by ultracentrifuge [35], several conflicting results have been reported in literature on arachin; these included whether the number of subunits in arachin is 12 [36] or six [37], or nine [38], whether the dissociation-association of arachin species is reversible [39] or there is an existence of different dissociation-association systems between dimmer and monomer [40], and whether the subunit composition of the two species (arachin I and arachin II) is the same, while containing four different kinds of subunits, with respect to molecular weight and six different kinds of subunits with respect to isoelectric points [37] or all the six subunits were distinguishable on PAGE [41]. The addition of SDS to arachin led to its dissociation into species of approximately one-sixth of its molecular weight [42] and later into several subunits [43].…”

Reconstitution of Arachin from Isolated Subunits

“…Arachin was purified from the dor-'mant seeds of groundnut (Arachis hypogaea L., a variety Hakuyu 7-3), as described in the previous paper. 6 ) Of the two molecular species of arachin, arachin I (mol. wt., 180,000) was used in this investigation.. Arachin was dissociated into subunits in 6M urea in the presence of 0.1 MtJ-mercaptoethanol, and the individual subunits were isolated by repeating the isoelectric focusing in a sucrose density gradient in the presence of 6M urea and 0.1 M tJmercaptoethanol, as described previously.…”