Dissociation between synaptic depression and block of long-term depression induced by raising the temperature in rat hippocampal slices

Young, Chainllie; Luo, Ming-Zen; Shen, Yu-Zen; Gean, Po‐Wu

doi:10.1002/1098-2396(200104)40:1<27::aid-syn1023>3.0.co;2-3

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…In rat hippocampal slices, it was reported that LFS at 1 Hz for 15 min became ineffective for the induction of LTD when the temperature of the perfusion medium increased from 32 to 39°C (Young et al, 2001). In the present study, we found that LFS of the same parameter was still effective for the induction of LTD in visual cortical slices when the temperature increased from 31 to 37°C.…”

Section: Temperature Is Not a Factor For The Difference In Effectivensupporting

confidence: 59%

Long-Term Depression Is Not Induced by Low-Frequency Stimulation in Rat Visual CortexIn Vivo: A Possible Preventing Role of Endogenous Brain-Derived Neurotrophic Factor

et al. 2003

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Low-frequency stimulation (LFS) at 1 Hz for 15 min is an effective protocol to induce homosynaptic long-term depression (LTD) in visual cortical slices. It is reported that LFS becomes ineffective when brain-derived neurotrophic factor (BDNF) is applied to slices. It is not known, however, whether such a protocol induces LTD in visual cortex in vivo, and whether endogenous BDNF has the same or similar action. To address these questions, we recorded field potentials of rat visual cortex evoked by stimulation of lateral geniculate nucleus, white matter, or cortical layer IV. We found that LFS did not induce LTD of cortical responses in vivo. To test the possibility that spontaneous activity from retinas would interfere with the induction of LTD, both eyes were removed or inactivated by tetrodotoxin. LTD was not induced in these conditions either. To test whether the difference in temperature between the two preparations is a factor for the discrepancy, the temperature of slices was increased from 31 to 37 degrees C. LTD was induced in slices at either temperature. Then, we hypothesized that endogenous BNDF and its receptors, TrkB, prevent the induction of LTD. To test this, we infused the cortex with an inhibitor of Trk receptor tyrosine kinases, anti-TrkB IgG1, anti-BDNF, and anti-neurotrophin 4/5 antibodies. LTD was induced when the BDNF-TrkB system was blocked. In slices, the level of phosphorylation of Trks was found to decrease with time. These results indicate that activation of TrkB signal pathway prevents LFS from inducing synaptic depression in visual cortex in vivo.

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Section: Temperature Is Not a Factor For The Difference In Effectivensupporting

confidence: 59%

Long-Term Depression Is Not Induced by Low-Frequency Stimulation in Rat Visual CortexIn Vivo: A Possible Preventing Role of Endogenous Brain-Derived Neurotrophic Factor

et al. 2003

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“…Hippocampus slices were transferred to a superfused recording chamber and allowed to stabilize for at least 30 min. Recording temperature was similar in the three groups tested (Controls: 29.7 ± 0.1°C; Ethanol: 29.9 ± 0.07; Pair‐fed: 29.7 ± 0.2; P > 0.05 between each group; unpaired Student‐ t test) and carefully maintained below 32°C since induction of LTD is impaired above this value (Young et al, ). All results were obtained 3–5 h after the dissection since it is the most efficient delay for triggering LTD (Billard, ).…”

Section: Methodsmentioning

confidence: 95%

Aberrant NMDA-dependent LTD after perinatal ethanol exposure in young adult rat hippocampus

Kervern¹,

Ferron²,

Alaux‐Cantin³

et al. 2015

Hippocampus

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Irreversible cognitive deficits induced by ethanol exposure during fetal life have been ascribed to a lower NMDA-dependent synaptic long-term potentiation (LTP) in the hippocampus. Whether NMDA-dependent long-term depression (LTD) may also play a critical role in those deficits remains unknown. Here, we show that in vitro LTD induced with paired-pulse low frequency stimulation is enhanced in CA1 hippocampus field of young adult rats exposed to ethanol during brain development. Furthermore, single pulse low frequency stimulation, ineffective at this age (LFS600), induced LTD after ethanol exposure accompanied with a stronger response than controls during LFS600, thus revealing an aberrant form of activity-dependent plasticity at this age. Blocking NMDA receptor or GluN2B containing NMDA receptor prevented both the stronger response during LFS600 and LTD whereas Zinc, an antagonist of GluN2A containing NMDA receptor, was ineffective on both responses. In addition, LFS600-induced LTD was revealed in controls only with a reduced-Mg(2+) medium. In whole dissected hippocampus CA1 field, perinatal ethanol exposure increased GluN2B subunit expression in the synaptic compartment whereas GluN2A was unaltered. Using pharmacological tools, we suggest that LFS600 LTD was of synaptic origin. Altogether, we describe a new mechanism by which ethanol exposure during fetal life induces a long-term alteration of synaptic plasticity involving NMDA receptors, leading to an aberrant LTD. We suggest this effect of ethanol may reflect a delayed maturation of the synapse and that aberrant LTD may also participates to long-lasting cognitive deficits in fetal alcohol spectrum disorder.

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Long-Term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multielectrode in Acute Brain Slice Preparations

Lukas

Holthoff

Egger

2018

Methods in Molecular Biology

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Single extracellular stimulation electrodes are a widespread means to locally activate synaptic inputs in acute brain slices. Here we describe the fabrication and application of a multielectrode stimulator that was developed for conditions under which independent stimulation of several nearby sites is desirable. For the construction of the multielectrode we have developed a method by which electrode wires can be spaced at minimal distances of 100 μm. This configuration increases the efficiency of stimulation paradigms, such as the comparison of proximal induced and control inputs for studies of synaptic plasticity.In our case the multielectrode was used for acute olfactory bulb slices to independently excite individual nearby glomeruli; the technique allowed us to demonstrate homosynaptic bidirectional long-term plasticity at the mitral/tufted cell to granule cell synapse. We also describe the determinants for successful recordings of long-term plasticity at this synapse, with mechanical and electrophysiological recording stability being tantamount. Finally, we briefly discuss data analysis procedures.

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Dissociation between synaptic depression and block of long-term depression induced by raising the temperature in rat hippocampal slices

Cited by 4 publications

References 40 publications

Long-Term Depression Is Not Induced by Low-Frequency Stimulation in Rat Visual CortexIn Vivo: A Possible Preventing Role of Endogenous Brain-Derived Neurotrophic Factor

Long-Term Depression Is Not Induced by Low-Frequency Stimulation in Rat Visual CortexIn Vivo: A Possible Preventing Role of Endogenous Brain-Derived Neurotrophic Factor

Aberrant NMDA-dependent LTD after perinatal ethanol exposure in young adult rat hippocampus

Long-Term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multielectrode in Acute Brain Slice Preparations

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