Distinct effects of sleep deprivation on binding to norepinephrine and serotonin transporters in rat brain

Hipólide, Débora Cristina; Moreira, Karin M.; Barlow, Karen; Wilson, Alan A.; Nóbrega, José N.; Tufik, Sérgio

doi:10.1016/j.pnpbp.2004.11.015

Cited by 59 publications

(32 citation statements)

References 34 publications

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“…A number of studies that have incorporated sleep deprivation in animals have explored the impact of potential cellular and molecular changes on learning, such as neurotransmitter levels (6), impairment of formation of long-term potentiation (cellular correlate of learning and memory; (7), and effects on levels of extracellular signal-regulated kinases (8). However, the molecular mechanism of changes in neuronal properties induced by sleep deprivation remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

The impact of sleep deprivation on hippocampal-mediated learning and memory in rats

Saygın¹,

Özgüner²,

Önder³

et al. 2017

BLL

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BACKGROUND: To examine the impact of different types of sleep deprivation on hippocampal-mediated learning and memory in rats. METHODS: Forty-eight Sprague-Dawley male rats were randomly assigned to 1 of 4 equal-size groups: (1) 12 hours of sleep per day (control). (2) total sleep deprivation (TSD), (3) rapid eye movement (REM) deprivation (RD), and (4) sleep restricted to 4 hours per day (SR). All rats were subjected to swimming training in the Morris water maze (MWM). At the end of the experiments, the rats were decapitated, and hippocampus tissue was analyzed for several neurotransmitters and receptors. RESULTS: The time spent at the target quadrant increased from 20.2 to 30.0 seconds in the control group on the third day of the experiment, whereas corresponding values increased from 20.2 to 21.8 seconds in the TSD group, 22.1 to 25.4 seconds in the RD group, and 21.2 to 32.0 sec in the SR group (p = 0.026). On the seventh day of the experiment, the values decreased to 25.0 seconds in controls, 22.5 in the RD group, and 23.6 in the SR group (p = 0.045). The TSD group demonstrated signifi cant decreases in glutamate and serotonin levels compared with the control group. There was a signifi cant increase in 5-HT2 a receptor expression in all intervention groups compared with the controls. CONCLUSIONS: Our results of glutamate levels and 5-HT2a receptor expression in the hippocampus seem to be primarily involved in sleep and memory regulation (Tab. 2, Fig. 4, Ref. 59). Text in PDF www.elis.sk.

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Section: Introductionmentioning

confidence: 99%

The impact of sleep deprivation on hippocampal-mediated learning and memory in rats

Saygın¹,

Özgüner²,

Önder³

et al. 2017

BLL

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“…5-HT is a neurotransmitter of the central and peripheral nervous system and modulates a variety of physiological processes such as sleep, mood, and appetite (Hipolide et al 2005;Serretti et al 2006;Jimerson et al 1990). After the release of 5-HT into the synaptic cleft, 5-HT is actively transported back into the presynaptic neurons by SERT.…”

Section: Introductionmentioning

confidence: 99%

Membrane Glycoprotein M6B Interacts with the Human Serotonin Transporter

2008

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The serotonin transporter (SERT) belongs to a family of sodium- and chloride-dependent neurotransmitter transporters that are responsible for the active re-uptake of the neurotransmitter serotonin from the synapse. In the present study, using the yeast two-hybrid system, we identified the membrane glycoprotein M6B as a binding partner of SERT. This interaction was further verified by co-immunoprecipitation and glutathione-S-transferase pull-down assays. M6B belongs to a proteolipid protein family, which is expressed in neurons and in oligodendrocytes in the brain. The knowledge of the biological function of this protein family is sparse, but their expression in most brain regions have led to the hypothesis that they are involved in cellular housekeeping functions such as membrane trafficking and cell-to-cell communication. The co-expression of SERT with M6B results in a significant decrease in SERT-mediated serotonin uptake caused by a down-regulation of SERT surface expression. Furthermore, we find, using confocal microscopy, that M6B co-localizes with SERT when transiently expressed in HEK-MSR-293 cells and when endogenously expressed in RN46A cells. Taken together, our data suggest that M6B regulates the serotonin uptake by affecting cellular trafficking of the serotonin transporter.

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“…Serotonin uptake sites in the rat brain have been described using a variety of selective inhibitors of the transporter, including [ 3 H]indalpine (Savaki et al 1985), [3H]cyanoimipramine (Kovachich et al 1988;Belej et al 1996), [ 3 H]paroxetine (DeSouza and Kuyatt 1987;Hrdina et al 1990;Chen et al 1992), [ 11 C]DASB (Hipolide et al 2005), and [ 3 H]citalopram (D'Amato et al 1987;Duncan et al 1992) (Table 2). Unlike the DAT, which is present in relatively low concentrations, the level of SERT binding in the amygdala is midway between that in the raphe nuclei, which contain the densest concentrations in the brain, and the cerebellum, which contains the least.…”

Section: Serotonin Transportermentioning

confidence: 99%

“…The overall brain distribution of the rodent NET has also been well characterized, but in many of these reports, once again, the amygdala has often been considered as a single structure or data has only been reported for the basolateral nucleus (Tejani-Butt 1992; Benmansour et al 2004;Hipolide et al 2005) (Table 3). In a more complete report, Sanders et al (2005) found that the relative densities of binding to the NET in the three subnuclei that were analyzed (lateral, basolateral, posterior cortical) were more or less equivalent.…”

Section: Norepinephrine Transportermentioning

confidence: 99%

“…NET;Hipolide et al 2005) binding sites in the rat brain at the level of the amygdala.Bl basal nucleus, lateral division; Bm basal nucleus, medial division; CEl central nucleus, lateral division; CEm central nucleus, medial division; Co cortical nucleus; L lateral nucleus. [ 3 H]nisoxetine image reproduced from Hipolide et al (2005) with permission from Elsevier Fig.…”

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confidence: 99%

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The comparative distributions of the monoamine transporters in the rodent, monkey, and human amygdala

Smith

Porrino

2008

Brain Struct Funct

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The monoamines in the amygdala modulate multiple aspects of emotional processing in the mammalian brain, and organic or pharmacological dysregulation of these systems can result in affective pathologies. Knowledge of the normal distribution of these neurotransmitters, therefore, is central to our understanding of both the normal processes regulated by the amygdala and the pathological conditions associated with monoaminergic dysregulation. The monoaminergic transporters have proven to be accurate and reliable markers of the distributions of their substrates. The purpose of this review was twofold: First, to briefly recount the functional relevance of dopamine, serotonin, and norepinephrine transmission in the amygdala, and second, to describe and compare the distributions of the monoamine transporters in the rodent, monkey, and human brain. The transporters were found to be heterogeneously distributed in the amygdala. The dopamine transporter (DAT) is consistently found to be extremely sparsely distributed, however the various accounts of its subregional topography are inconsistent, making any cross-species comparisons difficult. The serotonin transporter (SERT) had the greatest overall degree of labeling of the three markers, and was characterized by substantial inter-species variability in its relative distribution. The norepinephrine transporter (NET) was shown to possess an intermediate level of labeling, and like the SERT, its distribution is not consistent across the three species. The results of these comparisons indicate that caution should be exercised when using animal models to investigate the complex processes modulated by the monoamines in the amygdala, as their relative contributions to these functions may differ across species.

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Distinct effects of sleep deprivation on binding to norepinephrine and serotonin transporters in rat brain

Cited by 59 publications

References 34 publications

The impact of sleep deprivation on hippocampal-mediated learning and memory in rats

The impact of sleep deprivation on hippocampal-mediated learning and memory in rats

Membrane Glycoprotein M6B Interacts with the Human Serotonin Transporter

The comparative distributions of the monoamine transporters in the rodent, monkey, and human amygdala

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