Distinct expression profile of lncRNA in endometrial carcinoma

Abstract

Endometrial carcinoma (EC) is the most common malignancy in women. Dispite its prevalence, the prognosis of endometrial carcinoma still relies on conventional histological type, grade and invasion information. Its morbidity is still increasing and the outcome is very poor. To the best of our knowledge, hormonal imbalance and/or molecular genetic alterations are the main cause of EC. However, the alterations of lncRNAs which accounts for approximately 4/5 of human transcripts are still poorly understood. In the present study, using the RiboArray™ Custom Array, we studied the expression profiles of lncRNA in EC as compared to normal endometrium (NE) to find potential core lncRNAs for the diagnosis of EC. We found the potential core lncRNA by GO, KEGG, lncRNA and mRNA co-expression network. The potential functional lncRNAs were further detected by qPCR to validate the microarray results. A total of 172 lncRNAs and 188 mRNAs were found to be differentially expressed between type Ⅰ EC and the NE samples (fold change >1.5). qPCR validation showed good consistency with the microarray data. GO, pathway analysis, the lncRNA and mRNA co-expression network as well as the TCGA data revealed that 6 lncRNAs (KIAA0087, RP11-501O2, FAM212B-AS1, LOC102723552, RP11-140I24 and RP11-600K151) may be the core regulators of endometrial carcinogenesis. The potential core lncRNAs revealed by the mRNA and lncRNA co-expression network might be helpful to explore potential early diagnostic and therapeutic targets for EC.

Novel DNA methylation sites associated with cigarette smoking among African Americans

“…It is involved in cell growth, proliferation, and differentiation, and some classes of its protein are involved in cancer development [24]. KIAA0087 is a noncoding RNA gene associated with endometrial and pancreatic cancers [25]. Also, a non-coding RNA gene, MGAT3 (Mannosyl (Beta-1,4-)-Glycoprotein Beta-1,4-N-Acetylglucosaminy ltransferase) is involved in the function and biosynthesis of glycoprotein oligosaccharides.…”

Non-Coding RNAs and Endometrial Cancer

“…The expression of lncRNA and their role in defining EEC subgroups and their clinical aggressiveness must be defined further. Xu et al suggested that six lncRNAs may be the main regulators of endometrial carcinogenesis [ 38 ]. Xu measured a total of 172 lncRNAs and 188 mRNAs and reported that they were expressed differently in type I EC and the normal control samples [ 38 ].…”

Identification of long non‑coding RNA expression patterns useful for molecular‑based classification of type�I endometrial cancers

“…Nevertheless, mounting evidence has emphasized the roles and clinical significance of non-coding RNAs and, in particular lncRNAs, among others in endometrial cancer (33,34).…”