Distribution and antimicrobial resistance profile of coagulase-negative staphylococci from cattle, equipment, and personnel on dairy farm and abattoir settings

Gizaw, Fikru; Kekeba, Tolera; Teshome, Fikadu; Kebede, Matewos; Abreham, Tekeste; Hayishe, Halefom; Waktole, Hika; Tufa, Takele Beyene; Edao, Bedaso Mammo; Ayana, Dinka; Abunna, Fufa; Beyi, Ashenafi Feyisa; Abdi, Reta D.

doi:10.1016/j.heliyon.2020.e03606

Cited by 21 publications

(24 citation statements)

References 81 publications

(106 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another interesting nding in this research was that the highest AMR was found in isolates from humans and the environment, mainly against penicillins, cefoxitin, erythromycin and tetracyclines, being signi cantly higher in S. aureus. These results are similar to those reported in NAS and MRSA of human and environmental origin in dairy farms in different countries (Visciano et al 2014;Gizaw et al 2020). Other studies have found that isolates of S. aureus and NAS from environmental sources (milking parlor, canteens, cooling tank, among others) and from humans share the same AMR and virulence pro le with strains isolated from milk of cows with mastitis (Farias et al 2018;Krukowski et al 2020).…”

Section: Discussionsupporting

confidence: 91%

Antimicrobial resistance of Staphylococcus spp isolated in dairy farms and its relationship with the antimicrobial control and treatment of bovine mastitis

Rodríguez¹,

Gómez

2022

Preprint

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) of Staphylococcus strains from dairy farms has been attributed to the use of antibiotics in humans and animals. We conducted this research to identify the AMR genotypes and phenotypes of Staphylococcus spp isolates from different sources on dairy farms and their relationship with the control and treatment of mastitis. Antimicrobial susceptibility test was performed on 349 Staphylococcus isolates, identified by Vitek® 2 compact automated system and Maltitoff, from milk of cows with subclinical mastitis, teats of cows with and without mastitis, nasal mucosa of workers, and the environment of dairy farms. AMR genes were identified in S. aureus isolates by PCR. 152 S. aureus and 197 non-aureus staphylococci (NAS) showed resistance mainly to penicillin (54.1% and 32.4%) and ampicillin (50.3% and 27.0%), which was associated with the use of these antibiotics on farms. S. aureus isolated from workers and the environment exhibited significantly higher AMR compared to β-lactams. The identification of the blaZ (83.9%), aacA-aphD (48.6%), ermC (23.5%), tetK (12.9%) and mecA (11.8%) genes in S. aureus had low correspondence with the AMR phenotype. We conclude that the use of β-lactam antibiotics influences the expression of AMR on Staphylococcus circulating in dairy farms and that environmental and human isolates of S. aureus may be involved in the persistence of resistant strains in dairy farms.

show abstract

Section: Discussionsupporting

confidence: 91%

Antimicrobial resistance of Staphylococcus spp isolated in dairy farms and its relationship with the antimicrobial control and treatment of bovine mastitis

Rodríguez¹,

Gómez

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The consumption of milk in Ethiopia was reported to be between 17 and 19 liters per capita, which is lower than the regional average (for instance, the amount of milk consumed per capita in Uganda and Kenya is 50 and 90 liters, respectively) [ 12 ]. The production of milk in the country is lower than the requirement, and this might be due to versatile factors; including diseases, improper production of dairy animals, and milk processing practices in farms [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and antimicrobial susceptibility of Staphylococcus aureus in dairy farms and personnel in selected towns of West Shewa Zone, Oromia, Ethiopia

Banu

Gebremedhin

2022

PLoS ONE

View full text Add to dashboard Cite

Staphylococcus aureus is one of the foodborne disease-causing bacterial pathogens. A cross-sectional study was conducted in selected towns of the West Shewa Zone, Oromia, Ethiopia from December 2020 to April 2021. The objectives of the study were to estimate the occurrence and load of S. aureus in raw cows’ milk, the antimicrobial susceptibility patterns of the S. aureus isolates, and assess the knowledge, attitude, and practice of the farmers on factors of antimicrobial resistance. A total of 311 samples from raw cows’ milk (212), milkers’ hands (44), and milking buckets (55) swabs were collected and tested. The disc diffusion method was used to test the antimicrobial susceptibility of the isolates. A questionnaire survey was conducted to assess the factors of milk contamination with S. aureus and antimicrobial resistance. The Chi-square test, one-way analysis of variance, and logistic regression analysis were used for data analyses. The result indicated that 16.72% (52/311) (95% CI: 12.75–21.34%) of the samples were positive for S. aureus. The occurrence of S. aureus was 22.73%, 16.51%, and 12.73% in milkers’ hand swabs, cow milk, and milking bucket swabs, respectively. The mean count of S. aureus from raw cows’ milk was 4.3± 1.45 log10 CFU/ml. About 88% of S aureus isolates were resistant to ampicillin while 82.9% and 70.7% of the isolates were susceptible to ciprofloxacin and cefotaxime respectively. The majority of the S. aureus isolates (61%) showed multi-drug resistance. The odds of S. aureus isolation from the milk of cows were significantly high in older cows (Adjusted Odds Ratio [AOR]: 5.54; p = 0.001), in late lactation stages (AOR: 3.6; p = 0.012), and in farms where house cleaning was done twice per week (AOR: 8.7; p = 0.001). A high percentage of farmers had insufficient knowledge, attitude, and practice (KAP) about the factors contributing to antimicrobial resistance. In conclusion, the poor milk hygienic practices, high rate of antimicrobial resistance (AMR), and inadequate KAP of farmers about factors of AMR suggest potential public health risks thus requiring training and surveillance programs.

show abstract

“… 10 The CNS is of great importance due to its multidrug resistance nature. 12 In addition, studies showing the specific species of CNS in Ethiopia are scarce. Therefore, the current study was conducted with the objectives of estimating the prevalence of mastitis, assessment of potential risk factors associated with Zebu ( Bos indicus ) cows’ mastitis, isolation, and identification of Staphylococcus species, and determination of the antimicrobial susceptibility of the Staphylococcus aureus and CNS isolates.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Risk Factors of Mastitis and Isolation, Identification and Antibiogram of Staphylococcus Species from Mastitis Positive Zebu Cows in Toke Kutaye, Cheliya, and Dendi Districts, West Shewa Zone, Oromia, Ethiopia

Dabele¹,

Borena²,

Admasu³

et al. 2021

IDR

View full text Add to dashboard Cite

Purpose Mastitis is one of the major global problems severely affecting the dairy sector. Staphylococcus species are the primary bacteria consistently identified from mastitic milk. This study was aimed to estimate the prevalence of mastitis, isolate Staphylococcus species, determine risk factors, and the antimicrobial susceptibility profile of Staphylococcus species from mastitic Zebu cows in West Shewa Zone, Ethiopia. Materials and Methods A total of 404 lactating Zebu cows were tested for mastitis. Isolation and identification of Staphylococcus from mastitis positive samples were done by bacteriological culture and biochemical tests. Further identification of coagulase-negative Staphylococcus (CNS) species and antimicrobial susceptibility test of the Staphylococcus aureus and the CNS was done by the Phoenix machine. Descriptive statistics was used to summarize the prevalence of mastitis while the Chi-square test and logistic regression were used to determine the association between the prevalence of mastitis and the risk factors and the magnitude of association, respectively. Results The present study showed an overall cow and quarter level mastitis prevalence of 30.5% (95% confidence interval [CI]:26.0–35.2) and 8.3% (95% CI 7.0–9.8), respectively. The quarter level isolation rate of Staphylococcus species was 38.6% (95% CI: 30.1–47.6). Five Staphylococcus species namely S. intermedius, S. hyicus, S. aureus, S. lentus , and S. sciuri were identified. The latter two are CNS and were identified for the first time in Ethiopia. Antimicrobial susceptibility testing showed none of the isolates of S. aureus , 100% of S. sciuri , and 87.5% of S. lentus species were multidrug-resistant. The independent predictors of mastitis (p<0.05) were the age of the cows, stage of lactation, type of housing, the interval of bedding cleaning, and previous history of mastitis. Conclusion The study showed a high prevalence of mastitis, Staphylococcus species, and multidrug resistant S. lentus , and S. sciuri in Zebu cows.

show abstract

Distribution and antimicrobial resistance profile of coagulase-negative staphylococci from cattle, equipment, and personnel on dairy farm and abattoir settings

Cited by 21 publications

References 81 publications

Antimicrobial resistance of Staphylococcus spp isolated in dairy farms and its relationship with the antimicrobial control and treatment of bovine mastitis

Antimicrobial resistance of Staphylococcus spp isolated in dairy farms and its relationship with the antimicrobial control and treatment of bovine mastitis

Occurrence and antimicrobial susceptibility of Staphylococcus aureus in dairy farms and personnel in selected towns of West Shewa Zone, Oromia, Ethiopia

Prevalence and Risk Factors of Mastitis and Isolation, Identification and Antibiogram of Staphylococcus Species from Mastitis Positive Zebu Cows in Toke Kutaye, Cheliya, and Dendi Districts, West Shewa Zone, Oromia, Ethiopia

Contact Info

Product

Resources

About