Distribution of adipose-derived stem cells in adipose tissues from human cadavers

Mishra²,

et al. 2018

IJMS

101

Adipose-derived stromal/stem cells (ASCs) seems to be a promising regenerative therapeutic agent due to the minimally invasive approach of their harvest and multi-lineage differentiation potential. The harvested adipose tissues are further digested to extract stromal vascular fraction (SVF), which is cultured, and the anchorage-dependent cells are isolated in order to characterize their stemness, surface markers, and multi-differentiation potential. The differentiation potential of ASCs is directed through manipulating culture medium composition with an introduction of growth factors to obtain the desired cell type. ASCs have been widely studied for its regenerative therapeutic solution to neurologic, skin, wound, muscle, bone, and other disorders. These therapeutic outcomes of ASCs are achieved possibly via autocrine and paracrine effects of their secretome comprising of cytokines, extracellular proteins and RNAs. Therefore, secretome-derivatives might offer huge advantages over cells through their synthesis and storage for long-term use. When considering the therapeutic significance and future prospects of ASCs, this review summarizes the recent developments made in harvesting, isolation, and characterization. Furthermore, this article also provides a deeper insight into secretome of ASCs mediating regenerative efficacy.

Section: Introductionmentioning

confidence: 99%

Revisiting the Advances in Isolation, Characterization and Secretome of Adipose-Derived Stromal/Stem Cells

Mishra²,

et al. 2018

IJMS

101

Invest. Ophthalmol. Vis. Sci.

“…Kishi et al discovered that in human cadavers, fat tissues harvested from the thoracic back and lower abdomen had the highest concentration of CD34(þ)/CD31(À) ADSCs. 1,19 In our study, the cell markers CD31, CD34, CD45, CD73, CD90, CD105, and CD146 were used to identify ADSCs.…”

Section: Isolation and Culture Of Hoadscsmentioning

confidence: 99%

Orbital Volume Augmentation After Injection of Human Orbital Adipose-Derived Stem Cells in Rabbits

Lee

Shin

et al. 2013

“…Also adipose derived stem cells (ASCs) are available in adipose tissue (Kishi et al, 2010;Ulicna et al, 2010). ASCs in vivo are transformed into endothelial cells and they organize and develope angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue

Duman¹,

Aktan²,

Cüce³

et al. 2013

Int. J. Morphol.

SUMMARY:The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental step in the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and Cshowed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit® machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.