Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

Wang, L.P.; Hóng, Ní; Wang, G.P.; Xú, Wénxìng; Michelutti, R.; Wang, A.M.

doi:10.1016/j.cropro.2010.08.003

Cited by 25 publications

(12 citation statements)

References 38 publications

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“…BA is one of the most commonly used and effective cytokinins for developing axillary buds in both apple and pear [35] [36]. Other cytokinins such as Zea, 2iP and kinetin [37] are less commonly used, and the use of phenylurea derivatives such as thidiazuron and N-2-chloro-4-pyridyl)-N-phenylurea (CPPU) is sometimes described on shoot proliferation in pear cultures [38].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

Lizárraga¹,

Fraga²,

Ascasíbar³

et al. 2017

AJPS

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Section: Discussionmentioning

confidence: 99%

In Vitro Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

Lizárraga¹,

Fraga²,

Ascasíbar³

et al. 2017

AJPS

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“…Although the high temperatures applied over prolonged periods of time are more effective for virus eradication they also reduce the viability of the shoots cultured in vitro as well as their survival [15]. In our case the duration of heat Figure 2.…”

Section: Resultsmentioning

confidence: 98%

“…The viruses are transmissible by grafting and persist in the vegetative propagation material of infected trees being probably the main source of inoculums. There are neither known vectors nor other ways of natural transmission for these viruses [5] [6]. Therefore, cultivation of virus-free plants has the advantage of preventing other plants or fruit trees from being contaminated by these viruses.…”

Section: Introductionmentioning

confidence: 99%

“…Thermotherapy is most frequently performed at 36˚C -38˚C for 21 -35 days [14]. However, maintaining high temperatures for long periods of time has a negative effect on the survival and growth of meristems even though it is more effective in achieving high rates of virus-free plants [15]. New methods, like organogenesis, have tried to get higher rates from adventitious buds but they failed to eradicate viruses [16].…”

Section: Introductionmentioning

confidence: 99%

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Fast and Effective Thermotherapy Treatment for In Vitro Virus Eradication in Apple and Pear Trees

Lizárraga¹,

Ascasíbar²,

Fernández-González³

2017

AJPS

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Heat therapy followed by the isolation and in vitro culture of apical meristems is a suitable procedure for virus eradication. However, the period of heat treatment is usually long (28 -50 days) and the yield of viable plants free of viruses after treatment is often low (<50%). Here, we describe an alternative method to obtain virus-free plants. We used traditional Galician cultivars, six apple trees and two pear trees, infected with Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV). We combined heat therapy of in vitro shoots using a temperature gradient from 25˚C to 40˚C increasing 1˚C per day for a shorter period of time (18 days) with the posterior isolation and culture of apical meristems. All DNA samples analyzed, obtained from plants developed from meristems, were 100% free of ApMV and almost 90% free of ACLSV. With this in vitro procedure combined we obtained a good yield of tested plants free of viruses. Our method is fast and effective and it could be also useful to eradicate these and other viruses in other fruit trees.

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“…Some authors also reported that especially mixed infections of more than one virus are more diffi cult to eliminate (Knapp et al 1995;Da Câmara Machado et al 1998;Wang et al 2006;Paprstein et al 2008).…”

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confidence: 99%

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

Paprštein

Sedlák

Svobodová

et al. 2013

Hort. Sci. (Prague)

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Parštein F., Sedlák J., Svobodová L., Polák J., Gadiou S., 2013. Results of in vitro chemotherapy of apple cultivar Fragrance -Short communication. Hort. Sci. (Prague),.Th e eff ect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. Th e results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial fi eld-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with welldeveloped leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the fi rst chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. Th e success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity.

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Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

Cited by 25 publications

References 38 publications

<i>In Vitro</i> Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

<i>In Vitro</i> Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

Fast and Effective Thermotherapy Treatment for <i>In Vitro</i> Virus Eradication in Apple and Pear Trees

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

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Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

Cited by 25 publications

References 38 publications

&lt;i&gt;In Vitro&lt;/i&gt; Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

&lt;i&gt;In Vitro&lt;/i&gt; Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

Fast and Effective Thermotherapy Treatment for &lt;i&gt;In Vitro&lt;/i&gt; Virus Eradication in Apple and Pear Trees

Results of in vitro chemotherapy of apple cv. Fragrance - Short communication

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Resources

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<i>In Vitro</i> Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

<i>In Vitro</i> Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

Fast and Effective Thermotherapy Treatment for <i>In Vitro</i> Virus Eradication in Apple and Pear Trees