Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients

Khoshnood, Saeed; Shahi, Fatemeh; Jomehzadeh, Nabi; Montazeri, Effat Abbasi; Saki, Morteza; Mortazavi, Seyed Mojtaba; Maghsoumi‐Norouzabad, Leila

doi:10.1556/030.66.2019.015

Cited by 22 publications

(20 citation statements)

References 28 publications

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“…In southwest of Iran, in a hospital setting nearly 80% of the S. aureus strains isolated from burn patients were MRSA. 27 …”

Section: Discussionmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus Nasal Colonization Among Health Care Workers of a Tertiary Hospital in Ecuador and Associated Risk Factors

Baroja¹,

Guerra²,

Coral-Almeida

et al. 2021

IDR

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Background Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to most of the commonly used antibiotics and is therefore a public health issue. Colonization with MRSA is a risk factor for infection or transmission. Purpose To determine the prevalence of colonization with Staphylococcus aureus (SA) and MRSA strains in health care workers (HCWs) at a tertiary hospital in Ecuador and to determine the risk factors associated with carriage. Methods Out of a cohort of 3800 HCWs, 481 individuals from different hospital departments were randomly selected, and a single nasal swab was collected. Detection of SA and MRSA was carried out with the LightCycler ® MRSA Advanced Test. A questionnaire was performed that gathered demographic and occupational information of the participants to determine risk factors for MRSA colonization. Statistical analysis was performed with univariate and multivariate analysis and the R-software version 4.0.2. Results Colonization with SA and MRSA occurred in respectively 23.7% (95% CI, 22.7–24.6) and 5% (95% CI, 3.39–7.58) of the individuals. The multivariate analysis showed that being older in age (OD 1.09) and being male (OD 2.78) were risk factors for SA and MRSA colonization ( p -value < 0.001). Previous use of antibiotics or the use of nasal ointments diminished the colonization rates of SA (24% versus 3.7% and 10.1% respectively). Conclusion About 20% of the HCWs who were colonized with SA were colonized with MRSA, representing a risk for nosocomial infections and hospital outbreaks. Active monitoring and a decolonization treatment of the HCWs can reduce these risks.

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“…In southwest of Iran, in a hospital setting nearly 80% of the S. aureus strains isolated from burn patients were MRSA. 27 …”

Section: Discussionmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus Nasal Colonization Among Health Care Workers of a Tertiary Hospital in Ecuador and Associated Risk Factors

Baroja¹,

Guerra²,

Coral-Almeida

et al. 2021

IDR

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“…16 A previous study on MRSA in Khuzestan province revealed a dramatic annual increase in the proportion of isolates resistant to methicillin during the recent years, rising from 60% in 2017 and 2018 to a peak of 77% in 2019. [19][20][21][22] Such differences in the prevalence of MRSA infections in our region might reflect the fact that in these areas, different policies are considered for infection control and other factors. 23 There is a continuous increase in the global prevalence of MRSA in European countries.…”

Section: Discussionmentioning

confidence: 99%

Characterization of SCCmec, Spa Types and Multidrug Resistant of Methicillin-Resistant Staphylococcus aureus Isolates in Ahvaz, Iran

Moosavian¹,

Dehkordi²,

Hashemzadeh³

2020

IDR

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most pathogens associated with health care. Molecular typing methods are vital for outbreak investigations of MRSA. The aim of this study was characterization of SCCmec, spa types and multidrug resistant of methicillin-resistant Staphylococcus aureus isolates in Ahvaz, Iran. Methods: A total of 50 MRSA isolates were determined by using the phenotypic method and mecA gene. Antibiotic resistance profile and SCCmec types were screened using disc diffusion method and PCR, respectively. For spa typing of MRSA isolates, two molecular typing methods including the PCR-sequencing and high-resolution melting (HRM) analysis were used. Results: In the present study, the highest sensitivity of MRSA was to vancomycin and linezolid and the lowest to clindamycin. In the MRSA isolates, 22% were XDR and 78% were MDR. SCCmec type III was found commonly among MRSA. Based on PCR-sequencing and HRM results, 10 different spa types were identified. The spa types t037 and t030 were the most common in this study. Conclusion: This study emphasizes the spa variation among MRSA isolates, which may be considered as an important criterion when treating staphylococcal infections. Accurate and early detection of MDR, XDR, or even PDR MRSA isolates strains must be commenced by all clinical microbiology laboratories to reduce the menace of antimicrobial resistance.

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“…The proportions of isolates carrying this gene were nearly thrice as high as reported by Smith et al . [ 19 ] but still lower than the rates documented in staphylococcal infections (40–60%) [ 8 , 28 ]. In previous studies, mec A-carriage was frequently demonstrated in CoNS belonging to S. haemolyticus, S. epidermidis , and S. hominis species, which is consistent with our findings [ 5 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…oxacillin) and show insusceptibility to all β-lactams, including antibiotics with β -lactamase inhibitors and carbapenems. Furthermore, resistance to methicillin is frequently associated with the resistance to other groups of antibiotics (macrolides, lincosamides, aminoglycosides, fluoroquinolones, and sulfonamides), and requires the use of second-line antibiotics such as vancomycin, daptomycin, or linezolid [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Garbacz

Wierzbowska

Kwapisz

et al. 2021

Journal of Oral Microbiology

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Background: The use of antibiotics in dentistry is associated with the emergence and spread of antibiotic-resistant microorganisms, including commensal staphylococci. Methods: A total of 367 oral samples were collected, from which staphylococci were isolated and identified by using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF). The antibiotic susceptibility of the isolates was determined and molecular characteristics for methicillin-resistant staphylococci was performed. Results: A total of 103 coagulase-negative staphylococci (CoNS), among them S. warneri, S. haemolyticus, S. saprophyticus, S. pasteuri, S. epidermidis, S. hominis, S. xylosus, S. equorum, S. kloosii, S. succinus, S. cohnii , and S. simulans , were confirmed by MALDI-TOF. Resistance to most tested antibiotics was statistically higher in CoNS than in S. aureus isolates ( P -value < 0.05). CoNS isolates showed high resistance to penicillin ( S. saprophyticus 88.9%), erythromycin ( S. haemolyticus 84.6%), fusidic acid ( S. saprophyticus 77.8%), co-trimoxazole ( S. epidermidis 71.4%), gentamicin ( S. warneri 63.8%), and tetracycline ( S. saprophyticus 55.6%). Multidrug resistance was largely observed, especially among S. haemolyticus and S. saprophyticus species. Methicillin-resistance in S. haemolyticus (38.5%), S. saprophyticus (22.2%) and S. aureus (13.5%) was associated with the presence of the mec A gene and SCC mec type IV or V. Conclusion: Coagulase-negative staphylococci, especially S. haemolyticus and S. saprophyticus , seem to be a reservoir of methicillin resistance and multidrug resistance in the oral cavity.

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Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients

Cited by 22 publications

References 28 publications

Methicillin-Resistant Staphylococcus aureus Nasal Colonization Among Health Care Workers of a Tertiary Hospital in Ecuador and Associated Risk Factors

Methicillin-Resistant Staphylococcus aureus Nasal Colonization Among Health Care Workers of a Tertiary Hospital in Ecuador and Associated Risk Factors

<p>Characterization of <em>SCCmec</em>, <em>Spa</em> Types and Multidrug Resistant of Methicillin-Resistant <em>Staphylococcus aureus</em> Isolates in Ahvaz, Iran</p>

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

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