Distribution of mast-cell precursors in hematopoeitic and lymphopoietic tissues of mice.

Kitamura, Yukihiko; Shimada, M.; Go, Soei; Matsuda, Hiroshi; Hatanaka, Kenji; Seki, Masatoshi

doi:10.1084/jem.150.3.482

Cited by 121 publications

(51 citation statements)

References 16 publications

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“…Although controversy still exists concerning the precursors of mast cells and basophils, many investigators consider these cells to arise from a common stem cell. This view is supported by reports that indicate that mast cell precursors originate in the bone marrow, as is known for basophils (7,15 (Fig. 4).…”

supporting

confidence: 75%

Brief Communication

1996

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supporting

confidence: 75%

Brief Communication

1996

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“…Remarkably, the mast cells in the gastric mucosa stained only with alcian blue. The CFU-S represents a precursor of the mast cell lineage (32,47), but normal mouse peritoneal mast cells can exhibit extensive proliferative potential when injected intracutaneously into WBB6FI-W/W ~ mice (40). Taken together, these observations suggest that the alcian blue-positive mast cells in the gastric mucosa were actually derived from the injected peritoneal mast cells, and raises the possibility that, under certain circumstances, CTMC give rise to cells with at least some of the features of MMC.…”

Section: Discussionmentioning

confidence: 99%

“…Paraffin sections (10 #m) of tissues were made by routine methods and were stained with alcian blue-safranin. Mast cells in the skin, spleen, and glandular stomach were counted as described previously to determine whether successful transfer and development of mast cells had occurred (32,47). ~105 cells were examined in cytocentrifuge preparations of peritoneal cells after staining with alcian bluesafranin.…”

Section: Assay Of Spleen Colony-forming Units (Cfu-s) the Methods Of mentioning

confidence: 99%

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

Nakano¹,

Sonoda²,

Hayashi³

et al. 1985

The Journal of Experimental Medicine

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471

268

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Both connective tissue mast cells and mast cells grown in vitro are derived from multipotential hematopoietic stem cells, but these two mast cell populations exhibit many differences in morphology, biochemistry, and function. We investigated whether the phenotype of cultured mast cells or their progeny was altered when the cells were transferred into different locations in vivo. Cultured mast cells were immature by ultrastructure, and stained with alcian blue but with neither safranin or berberine sulfate, a fluorescent dye that binds to the heparin of connective tissue mast cell granules. By contrast, mast cells recovered from the peritoneal cavity of congenitally mast cell-deficient (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mice 10 wk after intraperitoneal injection of cultured WBB6F1-+/+ or C57BL/6-bgJ/bgJ mast cells stained with both safranin and berberine sulfate. Staining with berberine sulfate was prevented by treatment of the cells with heparinase but not chondroitinase ABC, suggesting that the adoptively transferred mast cell population had acquired the ability to synthesize and store heparin. Furthermore, the recovered mast cells were indistinguishable by ultrastructure from the normal mature peritoneal mast cells of WBB6F1-+/+ mice, and contained substantially more histamine than mast cells studied directly from culture. Intravenous injection of cultured mast cells resulted in the development of safranin-and berberine sulfate-positive mast cells in the peritoneal cavity, spleen, skin, and glandular stomach muscularis propria. Mast cells also developed on the glandular stomach mucosa, but these cells stained with alcian blue rather than safranin, and did not stain with berberine sulfate. This result suggests that cultured mast cells can give rise to mast cells of either the connective tissue type or mucosal phenotype, depending on anatomical location. Furthermore, transplantation of cultured mast cells into WBB6F1-W/Wv mice had no measurable effect on the anemia of the recipient mice, suggesting a possible strategy for repairing the mast cell deficiency of WBB6F1-W/Wv mice without affecting other bone marrow-derived populations such as erythrocytes. Intravenous injection of representative connective tissue type mast cells (30-50% pure peritoneal mast cells derived from WBB6F1-+/+ mice) gave results similar to those obtained with cultured mast cells: mast cells developing in the peritoneal cavity, skin, spleen, and glandular stomach muscularis propria of WBB6F1-W/Wv recipients stained with safranin and berberine sulfate, whereas mast cells developing in the mucosa of the glandular stomach stained only with alcian blue.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Origin of mast cell precursors were determined by using giant granules of bgJ/bg J mice as a marker (22,24). In one experiment, bone marrow cells of C57BL/6-bgJ/bg J mice were injected intravenously to nonirradiated WBB6F1-W/W v mice.…”

Section: Assay Of Spleen Colony-forming Unit (Cfu-s) the Methods Of Tmentioning

confidence: 99%

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Sonoda¹,

Kanayama²,

Hara³

et al. 1984

The Journal of Experimental Medicine

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Presence of mast cell precursors in the mouse peritoneal cavity was demonstrated, and the precursors were characterized. When a cell suspension, containing mast cell precursor(s), was directly injected into the skin of genetically mast cell-deficient WBB6F1 (WB X C57BL/6)-W/Wv mice, a cluster composed of approximately 2,000 mast cells appeared at the injection site. By determining the proportion of injection sites at which the mast cell cluster appeared, the concentration of mast cell precursors can be calculated by limiting dilution analysis. The concentration in the peritoneal cavity was about five times as great as the concentration in the bone marrow. Although peritoneal mast cell precursors were shown to originate from the bone marrow, physical characterization revealed that the peritoneal precursors differed from the marrow precursors. The peritoneal precursors were less susceptible to irradiation than the marrow precursors; the former were heavier than the latter. When a 95% pure mast cell suspension was prepared from the peritoneal cells by the removal of phagocytes and the density gradient centrifugation, 1 out of 16 cells had the potentiality to make a mast cell cluster in the skin of the W/Wv mice. Moreover, when a single mast cell was identified under the phase contrast microscope and picked up with the micromanipulator, 1 out of 17 mast cells made the cluster. This indicated that some peritoneal mast cells kept extensive proliferative potentiality even after morphological differentiation. In other words, some peritoneal mast cells themselves may function as the committed precursors.

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Distribution of mast-cell precursors in hematopoeitic and lymphopoietic tissues of mice.

Cited by 121 publications

References 16 publications

Brief Communication

Brief Communication

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

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