Distribution of phospholipase C isozymes in normal human lung tissue and their immunohistochemical localization

Hwang, Sung Chul; Park, Kwang Hwa; Ha, Mahn Joon; Noh, In Sook; Park, Tae Byung; Lee, Yi Hyung

doi:10.3346/jkms.1996.11.4.305

Cited by 7 publications

(4 citation statements)

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“…For instance, the rate of proteolytic degradation of protein coronas on gold nanoparticles (AuNPs) varied between different proteins, with half times (time required for degradation of 50% of the corona) ranging from around 4 to 70 hours, and this variation correlated with the degree of cell damage (Ma et al, 2015). Similarly, in our experiments, the delayed toxicity of Curosurf ® pre-incubated ZnONWs may be a result of the time required for the enzymatic degradation of the phospholipid corona, possibly by phospholipases, which are known to be present in lung tissue (Hwang et al, 1996). In type 2 alveolar epithelial cells, the degradation half time of internalized DPPC has been measured at around 2 hours (Fisher and Dodia, 1996).…”

Section: Discussionsupporting

confidence: 61%

Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells

et al. 2016

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Inhaled nanoparticles have high deposition rates in the alveolar region of the lung but the effects of pulmonary surfactant (PS) on nanoparticle bioreactivity are unclear. Here, the impact of PS on the stability and dissolution of ZnO nanowires (ZnONWs) was investigated, and linked with their bioreactivity in vitro with human alveolar epithelial type 1-like cells (TT1). Pre-incubation of ZnONWs with Curosurf® (a natural porcine PS) decreased their dissolution at acidic pH, through the formation of a phospholipid corona. Confocal live cell microscopy confirmed that Curosurf® lowered intracellular dissolution, thus delaying the onset of cell death compared to bare ZnONWs. Despite reducing dissolution, Curosurf® significantly increased the uptake of ZnONWs within TT1 cells, ultimately increasing their toxicity after 24h. Although serum, improved ZnONW dispersion in suspension similar to Curosurf®, it had no effect on ZnONW internalization and toxicity, indicating a unique role of PS in promoting particle uptake. In the absence of PS, ZnONW length had no effect on dissolution kinetics or degree of cellular toxicity, indicating a less important role of length in determining ZnONW bioreactivity. This work provides unique findings on the effects of PS on the stability and toxicity of ZnONWs, which could be important in the study of pulmonary toxicity and epithelial-endothelial translocation of nanoparticles in general.

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Section: Discussionsupporting

confidence: 61%

Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells

et al. 2016

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“…Moreover, although normal osteoblasts and low‐aggressive osteosarcoma cell lines (MG‐63 and SaOS‐2) share very similar expression panels with PLC enzymes, significant differences were identified in the expression of the most recently identified PLC η subfamily (Lo Vasco et al 2013). Based on literature data, the subcellular distribution of PLC enzymes seems to play a role in influencing their activity (Di Raimo et al 2016; Graber and Losa 1993; Hwang et al 1996; Staudt et al 2016). Therefore, given the involvement of PLC enzymes in a broad spectrum of physiopathological processes, we have investigated the presence of PLC in Extracellular Vesicles (EVs) produced by osteosarcoma cell lines.…”

Section: Introductionmentioning

confidence: 99%

Phosphoinositide-specific phospholipase C isoforms are conveyed by osteosarcoma-derived extracellular vesicles

Urciuoli

Leopizzi

Maio

et al. 2020

J. Cell Commun. Signal.

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Cancer cells are able to release high amounts of extracellular vesicles, thereby conditioning the normal cells in the surrounding tissue and/or in distant target organs. In the context of bone cancers, previous studies suggested that osteosarcoma cancer cells produce transforming extracellular vesicles able to induce a tumour‐like phenotype in normal recipient cells. Indeed, phosphoinositide‐specific phospholipase C (PI‐PLC) enzymes are differentially expressed in osteosarcoma cell lines with increasing aggressiveness, thus providing helpful insights to better define their role and functions in this bone tumour. By confocal microscopy analysis, we demonstrated that osteosarcoma‐derived extracellular vesicles convey all the assessed PI‐PLC isoforms, and that they localize into cell membrane bubble‐like structures, resembling extracellular vesicles about to be released, as conveyed and/or membrane protein. Cytofluorimetric analysis confirmed the presence of PI‐PLC isoforms in the extracellular vesicles collected from conditioned media of osteosarcoma cells. These findings suggest the feasibility to use circulating extracellular vesicles as biomarkers of osteosarcoma progression and/or the monitoring of this distressing disease.

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“…The panel of PLC expression was also modified by specific stimuli (61)(62)(63), including inflammation (64)(65)(66), or by some specific inhibitors (67,68). The subcellular distribution of the PLC enzymes influences their activity, suggesting specific roles for each isoform besides the cleavage of PIP2 (69)(70)(71)(72)(73). The PLC enzymes are involved in some human diseases, including the nervous system tumors (64,65) and abnormalities (64,(74)(75)(76)(77)(78)(79).…”

Section: ) (37)mentioning

confidence: 99%

Phosphoinositide Signal Transduction Pathway and Osteosarcoma Metastases

Vasco

Rita²

2021

Jentashapir J Cell Mol Biol

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: Metastasis spreading confers a worse prognosis to the clinical outcome among patients suffering from osteosarcoma, the most common primary bone tumor in childhood and adolescence. The detection of molecules involved in metastasis spreading might contribute to understanding tumor dissemination mechanisms, thereby opening the way to novel therapeutic strategies. The Ezrin-radixin-moesin (ERM) family proteins are activated after interacting with molecules belonging to the phosphoinositide signal transduction pathway. The phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule in the PI pathway, stabilizes the ERM proteins or a more efficient receptor binding. The PIP2 levels in the pathway are a critical element for regulating several cell events. The PIP2 levels are regulated using enzymes, including the PI-specific Phospholipase C family. A decrease in the PIP2 levels induces the dissociation of the ERM protein from the membrane. In this regard, the PI-PLC enzymes regulate this event. In this paper, the role of the PI signal transduction molecules in osteosarcoma metastases is discussed.

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Distribution of phospholipase C isozymes in normal human lung tissue and their immunohistochemical localization

Cited by 7 publications

References 0 publications

Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells

Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells

Phosphoinositide-specific phospholipase C isoforms are conveyed by osteosarcoma-derived extracellular vesicles

Phosphoinositide Signal Transduction Pathway and Osteosarcoma Metastases

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