2011
DOI: 10.15835/nsb315422
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Distribution of Trans-Anethole and Estragole in Fennel (Foeniculum vulgare Mill) of Callus Induced from Different Seedling Parts and Fruits

Abstract: In the present study, seeds from local cultivar of fennel were germinated on Murashige and Skoog medium (MS) without plant growth regulators. Different types of explants from the growing seedling such as cotyledonal leaves, hypocotyls, epicotyls and roots were cultured on MS medium, contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or with kinetin. Differential responses in the essential oil constituents were observed in the induction and development of callus. The major… Show more

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Cited by 24 publications
(16 citation statements)
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“…A quantity of 3 g of in vitro produced shoots in each treatment (control and TDZ treatments) was used for the extraction of volatile products by crushing with hexane (20 ml) for 2.5 h. The obtained solution was dried over anhydrous sodium sulphate (0.2 g per each sample) and then concentrated to 1 ml (Afify et al 2011).…”
Section: Extraction Of Volatile Productsmentioning
confidence: 99%
“…A quantity of 3 g of in vitro produced shoots in each treatment (control and TDZ treatments) was used for the extraction of volatile products by crushing with hexane (20 ml) for 2.5 h. The obtained solution was dried over anhydrous sodium sulphate (0.2 g per each sample) and then concentrated to 1 ml (Afify et al 2011).…”
Section: Extraction Of Volatile Productsmentioning
confidence: 99%
“…Herbal medicine is still the mainstay of about 65-80% of the whole population, mainly in developing countries, for primary health care because of better cultural acceptability, better compatibility with the human body and fewer side effects. Leaves, flowers, stems, roots, seeds, fruit and bark can all be constituents of herbal medicines (Afify et al, 2011a(Afify et al, , 2011b(Afify et al, , 2012aAli et al, 2011). The medicinal values of these plants lie in their phytochemical components which produce definite physiological actions on the human body (Afify and El-Beltagi, 2011).…”
Section: Introductionmentioning
confidence: 99%
“…Sorghum oil was significantly decreased after fermentation which improves the bioavailability of iron and zinc during soaking and germination of three white sorghum varieties (Afify et al, 2011a;Alemu, 2009). On the same time fennel were germinated on Murashige and Skoog medium (MS) without plant growth regulators reduce the amount of estragole (not favorite for Human consumption) and increase the amount of trans-anethole (Afify et al, 2011c).…”
Section: Resultsmentioning
confidence: 99%