Distributions of total and active bacteria in biofilms lining tubes of the onuphid polychaete Diopatra cuprea

Phillips, Tina; Lovell, Charles R.

doi:10.3354/meps183169

Cited by 25 publications

(22 citation statements)

References 18 publications

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“…Several earlier studies have indicated that the burrow wall can harbour greater number of bacterial cells than the ambient sediment (Papaspyrou et al, 2005;Kinoshita et al, 2008) and can function as a site for enhanced microbial metabolic activity (Phillips and Lovell, 1999). The phylogenetic analyses performed herein suggest that bioturbated sediment may also have higher diversity and species richness than ambient sediment and that the presence of U. deltaura or C. subterranea may result in shrimp-specific differences in bacterial community composition.…”

Section: Figurementioning

confidence: 67%

Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

et al. 2010

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Bioturbation is a key process in coastal sediments, influencing microbially driven cycling of nutrients as well as the physical characteristics of the sediment. However, little is known about the distribution, diversity and function of the microbial communities that inhabit the burrows of infaunal macroorganisms. In this study, terminal-restriction fragment length polymorphism analysis was used to investigate variation in the structure of bacterial communities in sediment bioturbated by the burrowing shrimp Upogebia deltaura or Callianassa subterranea. Analyses of 229 sediment samples revealed significant differences between bacterial communities inhabiting shrimp burrows and those inhabiting ambient surface and subsurface sediments. Bacterial communities in burrows from both shrimp species were more similar to those in surface-ambient than subsurface-ambient sediment (R ¼ 0.258, Po0.001). The presence of shrimp was also associated with changes in bacterial community structure in surrounding surface sediment, when compared with sediments uninhabited by shrimp. Bacterial community structure varied with burrow depth, and also between individual burrows, suggesting that the shrimp's burrow construction, irrigation and maintenance behaviour affect the distribution of bacteria within shrimp burrows. Subsequent sequence analysis of bacterial 16S rRNA genes from surface sediments revealed differences in the relative abundance of bacterial taxa between shrimp-inhabited and uninhabited sediments; shrimp-inhabited sediment contained a higher proportion of proteobacterial sequences, including in particular a twofold increase in Gammaproteobacteria. Chao1 and ACE diversity estimates showed that taxon richness within surface bacterial communities in shrimp-inhabited sediment was at least threefold higher than that in uninhabited sediment. This study shows that bioturbation can result in significant structural and compositional changes in sediment bacterial communities, increasing bacterial diversity in surface sediments and resulting in distinct bacterial communities even at depth within the burrow. In an area of high macrofaunal abundance, this could lead to alterations in the microbial transformations of important nutrients at the sediment-water interface.

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Section: Figurementioning

confidence: 67%

Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

et al. 2010

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“…In terms of physico-chemical characteristics, infaunal burrow walls are fairly stable environments compared to the more frequently disturbed sediment/water interface (Steward et al 1996). This stability over time allows for the development of complex microbial biofilms (Steward et al 1996, Phillips & Lovell 1999. It leads to the formation of microbial communities fundamentally different from the nearby surface and subsurface sediments, with substantial bacterial diversity and high bacterial densities and growth rates (Steward et al 1996, Dobbs & Guckert 1988a.…”

Section: Discussionmentioning

confidence: 99%

“…Studies analyzing how marine deposit-feeders affect microbial variables focused on diverse species of crustaceas, holothurias and molluscs. Few reports deal with polychaetes (Aller & Yingst 1985, Duchene et al 1988, Reichardt 1988, Plante et al 1989, Plante & Jumars 1993, Steward et al 1996, Plante & Shriver 1998, Phillips & Lovell 1999. Among polychaetes, data dealing with the deposit-feeder and borrowing Hediste (Nereis) diversicolor (O.F.…”

Section: Introductionmentioning

confidence: 99%

“…A few studies have examined the impacts of depositfeeding infauna on microbial community structure using either cultivation techniques (Findlay & White 1983, Duchêne et al 1988 or analysis of phospholipid fatty acid (PLFA) profiles (Dobbs & Guckert 1988a,b, Steward et al 1996, Phillips & Lovell 1999, Bird et al 2000. Cultivation techniques have drawbacks because isolates typically represent less than 1% of natural bacterial communities (Amann et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of free-living and attached bacteria in sediments colonized by Hediste diversicolor

Lucas¹,

Bertru²,

Höfle³

2003

Aquat. Microb. Ecol.

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The dynamics of free-living and attached bacteria populations were studied in salt marsh sediments that were extensively colonized by the polychaete Hediste (Nereis) diversicolor (O. F. Müller). Bioturbation by H. diversicolor affected the density and community structure of free-living (FLB) and attached (AB) bacteria assemblages. Passage through the polychaete gut resulted in an 81 to 88% decrease of both bacterial groups and shift in favor of FLB in the hindgut (HG). In surficial sediments outside and inside the worm bed, attached bacteria represented 77 to 99% of the total bacterial densities. Although FLB were minor in terms of biomass, their densities strongly increased in January and May and peaked from 9.6 to 16.2 × 10 8 cells g -1 . AB peaked in August and October with densities from 205.8 to 283.6 × 10 8 cells g -1. AB densities were significantly higher in burrow wall sediment than in surrounding sediment. The density of FLB and AB was reduced in surficial sediment inside the worm bed compared to sediments not colonized by H. diversicolor. Community fingerprints of environmental 5S rRNA showed that there was no significant difference between the structure of the 2 bacterial assemblages. However, in August 1995, the 2 assemblages were highly divergent in the sediment outside the worm bed. The structure of FLB and AB from inside the worm bed was significantly different from the structure of bacterial assemblages outside the sediments colonized by H. diversicolor.

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“…Although they are known as biogeochemical hot-spots with high organic loading, bacterial numbers, and activity (Kristensen 1988, Reichardt 1988, Hansen et al 1996, Phillips & Lovell 1999, relatively few studies have quantified and located biogeochemical reactions in the vicinity of the infaunal burrows. Direct measurements in the sediment immediately surrounding burrow structures have shown that this unique environment increases oxygen consumption (Binnerup et al 1992), hampers sulfate reduction rates (Gribsholt et al 2003, Nielsen et al 2003 and increases potential nitrification and denitrification (Kristensen 1985, Mayer et al 1995 compared to the surrounding sediment.…”

Section: Introductionmentioning

confidence: 99%

Microscale distribution of oxygen and nitrate in sediment inhabited by Nereis diversicolor: spatial patterns and estimated reaction rates

Nielsen

Gribsholt

Kristensen³

et al. 2004

Aquat. Microb. Ecol.

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Profiles of oxygen and nitrate were measured with microsensors in sediment surrounding burrow structures of the polychaete Nereis diversicolor in a shallow mudflat in Limfjorden, Denmark. Rates and spatial distribution of reactions involving oxygen and nitrate (oxygen consumption, nitrification and nitrate reduction) in the vicinity of burrow structures and surface sediment were calculated and identified from concentration profiles. The burrow walls of the natural N. diversicolor population increased the surface area of the sediment -water interface several-fold and introduced oxic microniches with high metabolic activity into otherwise anoxic sediment. Nitrification and nitrate reduction were spatially separated processes, with nitrification restricted to the 0.9 to 1.5 mm thick oxic zone and nitrate-reduction occurring as a strictly anaerobic process in the nitratecontaining layers next to the oxic zone. At a density of 2560 individuals m -2 , burrow walls of N. diversicolor account for 12 to 40% of bulk sediment oxygen uptake and 50 to 77% and 58 to 82% of bulk nitrification and nitrate reduction, respectively. Thus, burrow structures of macro-infauna are potentially a major sediment compartment involved in nitrogen cycling and may promote loss of bioavailable nitrogen from the sediment.

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Distributions of total and active bacteria in biofilms lining tubes of the onuphid polychaete Diopatra cuprea

Cited by 25 publications

References 18 publications

Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

Characterization of free-living and attached bacteria in sediments colonized by Hediste diversicolor

Microscale distribution of oxygen and nitrate in sediment inhabited by Nereis diversicolor: spatial patterns and estimated reaction rates

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