Disulfide Cross-Linked Network Structure of Intermediate Filament and Matrix in Hair and Wool Cortices

Suzuta, Kazuyuki; Arai, Kozo

doi:10.2115/fiber.71.237

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…When wool fibers were dissolved and regenerated to form keratin film (KF), the diffraction peaks corresponding to crystal structure (α-helix structure and β -sheet structure) were remarkably reduced, and the proportion of amorphous region was increased. It was obviously indicated that the secondary structure of part of the keratin molecular chain was destroyed during the fiber dissolution process [35]. These molecular chains were disorderedly entangled together and eventually formed the amorphous region in the KF sample.…”

Section: Resultsmentioning

confidence: 99%

Effects of Graphene Oxide on the Structure and Properties of Regenerated Wool Keratin Films

Yang

Niu

et al. 2018

Polymers

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Much research has focused on improvement of the structural and mechanical properties of regenerated keratin materials by physical or chemical methods in recent years. In this research, regenerated keratin materials were modified with graphene oxide (GO). The properties of modified keratin films and the mechanism of interaction between GO and keratin macromolecules were studied. The SEM and XRD test results showed that the orientation of keratin macromolecules could be effectively improved by GO, which favored improvement of the keratin material’s crystallinity and made the films more uniform and compact. The thermal stability and mechanical properties of GO-modified keratin films were also improved significantly. At the same time, the reaction mechanism between keratin and GO materials was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), FT-IR, and Raman spectroscopy. It was shown that there was no chemical reaction between GO and keratin molecules, and the interaction between them was mainly via hydrogen bonding and van der Waals forces.

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Section: Resultsmentioning

confidence: 99%

Effects of Graphene Oxide on the Structure and Properties of Regenerated Wool Keratin Films

Yang

Niu

et al. 2018

Polymers

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“…The disulfide bonds in cystine form a three-dimensionally linked network together with salt type bonds and hydrogen bonding. 9,10 It is well known that disulfide bonds have great bonding energy, making keratin's secondary structure difficult to damage easily, 11 so the wool fiber has higher stability and lower solubility. How to effectively break the disulfide bonds in the dissolving process while at the same time ensuring the protein macromolecule structure is not damaged or degraded too much is the key to obtaining high molecular weight keratin solution.…”

mentioning

confidence: 99%

Study on the effect of organic phosphonic compounds on disulfide bonds in wool

Yang

Niu

et al. 2018

Textile Research Journal

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In this study a new type of organic phosphonic compound named LKS-610, a kind of reducing agent, was used to cleave disulfide bonds in wool fibers. Research into the extraction of keratin from wool and processing into useful textile materials is a hot topic in the field of textiles. Wool fibers are rich in disulfide bonds which are the main chemical crosslinkage to maintain the fiber's secondary structure. If the disulfide bonds between molecular chains can be sufficiently disrupted, it will facilitate to obtain the keratin solution suitable for the regenerated materials preparation. The reaction mechanism and effect were analyzed through scanning electron microscopic test (SEM), X-ray photoelectron spectroscopy test (XPS) and amino acid content test. After a period of processing time, the valence state of sulfur element on fiber surface changed significantly; this result shows that the disulfide bonds reacted with LKS-610 reagent and generated thiol groups. Amino acid analysis demonstrated that for wool fibers treated for 60 min at 80 C, the quality percentage of cystine in wool was reduced from 10.09% to 1.05%, the majority of disulfide bonds had been cleaved. In this case, fiber scales were almost completely stripped and the cortical layer was not significantly damaged but became prone to swelling. This indicated that LKS-610 reagent could disrupt the disulfide bonds in the wool thoroughly and efficiently, and reduce these bonds to thiol groups. Wool fibers pretreated by LKS-610 reagent can be dissolved readily to prepare keratin solution; this keratin solution with high dissolution ratio and large molecular weight can be used in research into modification and preparation of regenerated protein materials.

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“…The perm treatment process consists of two steps, the breaking of disulfide bonds in cysteine of protein by reducing agents and the restructuring of the disulfide bonds by oxidation. [9][10][11] Inspired by perm, it is a green and cleaner method to construct crosslinking structure based on disulfide bonds instead of adding the crosslinking agent. Disulfide bond is a chemical bond that drives from the sulfhydryl groups of two different cysteine residues in different peptide chains or the same peptide chain.…”

Section: Doi: 101002/mame202100498mentioning

confidence: 99%

Perm‐Inspired High‐Performance Soy Protein Isolate and Chicken Feather Keratin‐Based Wood Adhesive without External Crosslinker

Zhou

et al. 2021

Macro Materials & Eng

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Crosslinking modification can effectively improve the water resistance of soy protein isolate (SPI) adhesive, but it often depends on petroleum-based reagents, violating the concept of green environmental protection. Here, inspired by the breaking and recombination of the disulfide bond in the perm process, a high-performance wood adhesive is prepared by incorporation of SPI (modified by sodium sulfite to cleave the disulfide bonds of protein chains) and feather keratin (FK, extracted from waste chicken feathers by breaking the disulfide bond) without using any other crosslinkers. The crosslinking reaction occurs by disulfide bonds derived from the sulfhydryl group of FK and SPI. Thus the wet shear strength of the SPI/FK-20 adhesive is improved from 0.57 to 1.18 MPa, an increment of 107%. This study provides a green strategy to prepare high-performance protein-based adhesive from the waste products-chicken feathers, which will contribute to the development of the environmentally friendly wood adhesive industry.

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Disulfide Cross-Linked Network Structure of Intermediate Filament and Matrix in Hair and Wool Cortices

Cited by 4 publications

References 23 publications

Effects of Graphene Oxide on the Structure and Properties of Regenerated Wool Keratin Films

Effects of Graphene Oxide on the Structure and Properties of Regenerated Wool Keratin Films

Study on the effect of organic phosphonic compounds on disulfide bonds in wool

Perm‐Inspired High‐Performance Soy Protein Isolate and Chicken Feather Keratin‐Based Wood Adhesive without External Crosslinker

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