Disulfide‐linked surface molecules of monoclonal antigen‐specific suppressor T cells: evidence for T cell receptor structures

Ballinari, Dario; Castelli, Chiara; Traversari, Catia; Parmiani, Giorgio; Palmieri, Gabriella; Ricciardi‐Castagnoli, Paola; Adorini, Luciano

doi:10.1002/eji.1830150822

Cited by 13 publications

(3 citation statements)

References 41 publications

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“…Suppression may be mediated by many diverse cell types (8)(9)(10)(11)25) and by a variety of soluble products (10,25,26 (27,28). In other cases heterodimeric polypeptides have not been identified (29,30 It is interesting to speculate that the receptor polypeptides have similar molecular masses to murine 'y-8 heterodimeric proteins (21)(22)(23)(24).…”

Section: Discussionmentioning

confidence: 99%

CD3-associated heterodimeric polypeptides on suppressor hybridomas define biologically active inhibitory cells.

Weiner

Hanna²,

Bluestone³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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We have investigated the relationship between CD3 expression and the suppressor T-cell function. We have isolated stable clonal cell lines of the F12.23 suppressor T-cell hybridoma that are either CD3+ or CD3-. These lines were subjected to functional assays including inhibition of in vivo hapten-specific delayed-type hypersensitivity responses, in vitro hapten-specific interleukin 2 responses, as well as haptenspecific cytotoxic T-lymphocyte assays. In all assays, the functional suppressor phenotype absolutely correlated with CD3 surface expression. Furthermore, we have immunoprecipitated heterodimeric proteins that share molecular features with some receptor polypeptides previously described. CD3 polypeptides found on the surface of suppressor T cells are phosphorylated after phorbol ester stimulation. Collectively these studies unambiguously define the suppressive supernatant function as a product of CD3+ receptor-bearing T cells.

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Section: Discussionmentioning

confidence: 99%

CD3-associated heterodimeric polypeptides on suppressor hybridomas define biologically active inhibitory cells.

Weiner

Hanna²,

Bluestone³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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“…Characterization of the two cell lines was not limited to phenotype, but also concerned the fine antigen specificity and the immunological function of the cells and their soluble products (Adorini et al, , 1983aRicciardi-Castagnoli et al, 1981Sinigaglia et al, 1984). We also previously showed that disulfide-linked molecules of 42 kd were revealed by two-dimensional gel electrophoresis of radiolabeled LH8 cell surface proteins under non-reducing/reducing conditions (Ballinari et al, 1985). We also previously showed that disulfide-linked molecules of 42 kd were revealed by two-dimensional gel electrophoresis of radiolabeled LH8 cell surface proteins under non-reducing/reducing conditions (Ballinari et al, 1985).…”

Section: Gene Rearrangementmentioning

confidence: 92%

Rearrangement and expression of the antigen receptor alpha, beta and gamma genes in suppressor antigen-specific T cell lines.

Mori¹,

Lecoq²,

Robbiati³

et al. 1985

The EMBO Journal

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The rearrangement and transcription of the antigen receptor alpha, beta and gamma genes were investigated in murine antigen‐specific suppressor T cell lines, to establish whether the suppressor T cell subset expresses the same antigen receptor transcripts previously found in helper and cytotoxic T lymphocytes. The genomic organization of the alpha, beta and gamma chain loci was investigated using probes representative of the entire gene or fragments from variable, joining and constant regions. The present results show that in functional suppressor T cells the three antigen receptor genes are all rearranged. The beta gene is expressed in all the tested cell lines, while the expression of the alpha and gamma genes is variable. In one cell line (LH8) alpha and gamma genes are not efficiently transcribed; in the other cell line (LA41) the gamma mRNA is found in amounts similar to beta mRNA, whereas the alpha gene is expressed at low levels. These data suggest that in suppressor T cells no direct correlation exists between the expression of alpha, beta and gamma antigen receptor genes and the effector function.

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“…However, the subunit composition ofthe antigen receptor on suppressor T cells (Ts) remains to be solved; several investigators have reported discrepant results, demonstrating that some Ts lines and hybridomas express a-and p-chain genes, whereas some do not (5)(6)(7)(8)(9) despite having their Ts function. Thus, two points should be discussed in this regard.…”

mentioning

confidence: 99%

Sequence and expression of transcripts of the T-cell antigen receptor alpha-chain gene in a functional, antigen-specific suppressor-T-cell hybridoma.

Imai¹,

Kanno²,

Kimoto³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

103

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We have detected rearrangement and expression of a gene encoding a T-cell antigen receptor a chain in the keyhole limpet hemocyanin (KLH)-specific, inducible suppressor-T-cell (TJ) hybridoma 34S-281 (BW5147 lymphoma-C57BL/6 TJ) by using a-chain cDNA clones isolated from this T, hybridoma. The cDNA clones have a restriction length polymorphism in the constant region that identifies them as being of C57BL/6 origin. The cDNA sequence has an ATG start codon for an open reading frame including variable, joining, and constant gene segments. Furthermore, the Ts a-chain gene transcripts were detected on membrane-bound polysomes by RNA blot analysis using a variable-region fragment from one of the a-chain cDNA clones as probe, suggesting that they are actively translated in the Ts hybridoma. Ca Probe. A cDNA clone (Ta-802) encoding the V-J-C a-chain message was isolated from a C57BL/6 thymus cDNA library by screening with three synthetic oligodeoxynucleotides (21-mers) corresponding to the C region of a-chain gene. A 590-base-pair (bp) Nco I fragment from this clone was used as the C, probe (Fig. 2b). DNA Blot Analysis. DNA was digested with restriction enzymes and separated by electrophoresis according to standard methods (16). DNA was blotted to nitrocellulose by the procedure of Southern (17). After hybridization, the filters were washed with 2 x SSC/0.1% NaDodSO4 ( x SSC is 0.15 M NaCl/0.015 M sodium citrate) at 65°C and then with 0.1x SSC/0.1% NaDodSO4 at 650C. Air-dried filters were exposed at -80°C to Kodak XAR-5 film with intensifying screens for one to several days. Long exposures were carried out for at least 1 week.

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Disulfide‐linked surface molecules of monoclonal antigen‐specific suppressor T cells: evidence for T cell receptor structures

Cited by 13 publications

References 41 publications

CD3-associated heterodimeric polypeptides on suppressor hybridomas define biologically active inhibitory cells.

CD3-associated heterodimeric polypeptides on suppressor hybridomas define biologically active inhibitory cells.

Rearrangement and expression of the antigen receptor alpha, beta and gamma genes in suppressor antigen-specific T cell lines.

Sequence and expression of transcripts of the T-cell antigen receptor alpha-chain gene in a functional, antigen-specific suppressor-T-cell hybridoma.

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