Diurnal variation in degradation of phytic acid by plant phytase in the pig stomach

Kemme, P.A.; Jongbloed, A.W.; Mroz, Z.; Beynen, A.C.

doi:10.1016/s0301-6226(97)00163-2

Cited by 33 publications

(30 citation statements)

References 28 publications

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“…In addition, the non-enzymatic degradation of IP6 in the stomach can be considered as negligible, because IP6 molecule is very stable when exposed to gastric pH condition (Kemme et al, 1998).…”

Section: Fluctuation Of Post-prandial Plasma Mineral Level Of Juvenilmentioning

confidence: 99%

FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION

Laining

Rachmansyah

Lideman³

et al. 2010

Indonesian Aquaculture Journal

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In order to investigate the phytic acid degradation in the gut of post juvenile Japanese flounder, indirect method was carried out by measuring the pre-prandial and postprandial plasma mineral and alkaline phosphatase (ALP) level as well as liver phosphorus content. The experiment was designed into a Randomized Block in which experiment units were grouped according to sampling days at 10, 20 and 30 days of feeding time. Experimental diets contained three levels of dietary inorganic phosphorus at 0.0; 0.25 and 0.5% combined with two levels of dietary phytase at 0 and 2000 FTU/kg diet. Juvenile Japanese flounder (IBW = 36.2 g) were randomly distributed into 6 tanks of a 200 L capacity with density of 15 fish/tank. Blood sampling was carried out at 0 hour (before feeding or pre-prandial) and at 1, 3, 6 and 12 hour post feeding (post-prandial) time in three sampling days, respectively. Plasma was measured for mineral and ALP levels, while liver was analyzed for P content. The observation showed that fish fed without both dietary IP and phytase supplements had the lowest postprandial plasma IP, Mg and ALP levels during 12-h postprandial period. Plasma IP level at 6-h post-feeding in groups fed dietary 0.25 and 0.5% IP were significant higher when diet supplemented with phytase than those without phytase supplement. Peak level of plasma IP in fish fed 0.25% IP was similar to fish fed 0.5% with the presence of dietary phytase. At 1 and 3-h post-feeding, plasma Ca level increased in all groups, but significant difference was only observed between group fed diet without both dietary IP and phytase and other groups. Similar to plasma IP level, peak of plasma Mg and ALP concentration occurred in fish fed 0.25% IP together with phytase, and did not significantly differ from fish fed with 0.5% IP even when phytase was included in diet.

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Section: Fluctuation Of Post-prandial Plasma Mineral Level Of Juvenilmentioning

confidence: 99%

FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION

Laining

Rachmansyah

Lideman³

et al. 2010

Indonesian Aquaculture Journal

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“…Thus, in theory degradation of InsP 6 has to be optimised before digesta enters the small intestine. In dry fed pigs InsP 6 is mainly degraded by phytase in the stomach (Kemme et al, 1998;Rapp et al, 2001), where the retention time of the diet is limited and thereby the time for InsP 6 degradation. On the other hand, when feed and water are mixed before feeding, InsP 6 degradation by phytase is initiated (Carlson and Poulsen, 2003;Blaabjerg et al, 2007), and may continue in the pig's stomach.…”

Section: Introductionmentioning

confidence: 99%

Heat-treatment, phytase and fermented liquid feeding affect the presence of inositol phosphates in ileal digesta and phosphorus digestibility in pigs fed a wheat and barley diet

Blaabjerg

Jørgensen

Tauson

et al. 2010

Animal

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The aim was to evaluate the effect of heat-treatment, microbial phytase addition and feeding strategy (dry feeding v. fermented liquid feeding) on degradation of phytate (myo-inositol hexakisphosphate, InsP 6 ) and formation and further degradation of lower inositol phosphates (myo-inositol pentakisphosphate-myo-inositol bisphosphate, InsP 5 -InsP 2 ) at the distal ileum of pigs. Furthermore, the apparent ileal digestibility/degradability (AID) of phosphorus (P), InsP 6 -P and calcium (Ca) and the apparent total tract digestibility (ATTD) of P and Ca were studied. Pigs were fitted with a T-shaped ileal cannula for total collection of digesta at 2 h intervals during an 8 h sampling period after feeding the morning meal. Each period lasted for 2 weeks: 8 days of adaptation followed by 3 days of total collection of faeces and 3 days of total collection of ileal digesta. The experiment was designed as a 4 3 4 Latin square with four pigs fed four diets. A basal wheat/barley-based diet was fed either as nonheat-treated or heat-treated (steam-pelleted at 908C). The heat-treatment resulted in an inactivation of plant phytase below detectable level. Diet 1 (non-heat-treated basal diet fed dry); diet 2 (heat-treated basal diet fed dry); diet 3 (as diet 2 but with microbial phytase (750 FTU/kg as fed) fed dry); diet 4 (as diet 3 fed liquid (fermented for 17.5 h nighttime and 6.5 h daytime at 208C with 50% residue in the tank)). Chromic oxide (Cr 2 O 3 ) was included as marker and ATTD was determined both by total collection of faeces (ATTD Total ) and Cr 2 O 3 (ATTD Cr ). InsP 6 was completely degraded in diet 4 before feeding resulting in no InsP 6 -P being present in ileal digesta. InsP 6 -P concentration in ileal digesta decreased with increasing dietary levels of plant or microbial phytase in pigs fed the dry diets. Consequently, AID and ATTD of P and Ca were greatest for pigs fed diet 4 followed by diets 3, 1 and 2. The ATTD of P depended on the used method as ATTD Total of P was 72%, 61%, 44% and 34%, whereas ATTD Cr of P was 65%, 52%, 38% and 23% for diets 4, 3, 1 and 2, respectively. In all pigs the ileal concentration of InsP 5 -InsP 2 -P was extremely small, and thus unimportant for maximisation of ATTD of plant P. In conclusion, fermented liquid feeding with microbial phytase seems to be an efficient approach to improve ATTD of plant P compared with dry feeding. This opens up for further reductions in P excretion.

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“…A maioria das plantas apresenta atividade fitásica, mas o nível e a ação nos grãos variam entre os cereais (Barrier-Guillot et al, 1996). O triticale, por exemplo, apresenta alta atividade fitásica endógena, que pode aumentar a degradação do ácido fítico em 97% em relação às dietas à base de milho e farelo de soja para suínos (Kemme et al, 1998). Neste estudo, entretanto, não foi observado efeito do triticale sobre a digestão do fósforo.…”

Section: Resultsunclassified

Utilização do triticale e de enzimas em dietas para suínos: digestibilidade e metabolismo

Hauschild

Lovatto

Lehnen

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliou-se o efeito da inclusão de níveis de triticale sobre o valor nutritivo de dietas para suínos com ou sem enzimas. Foram utilizados 24 suínos machos, castrados, com peso inicial de 59kg, alojados em gaiolas metabólicas. O delineamento foi de blocos ao acaso em arranjo fatorial 3 x 2 (níveis de triticale, 0, 30 e 60%, com ou sem enzimas), com quatro repetições cada. A inclusão de triticale na dieta em até 60% e a adição de enzimas não influenciou (P>0,05) a digestibilidade da matéria seca, fósforo, energia bruta, metabolização da energia, energia digestível e metabolizável e o balanço do N. Os valores médios de energia digestível e metabolizável foram de 3.537 e 3.435kcal. Houve interação triticale x enzimas na proteína digestível aparente (PD a ) (P<0,01). Na dieta com 60% de triticale sem adição de enzimas a PD a foi 6% inferior à controle. Na dieta com 30 e 60% de triticale com enzimas, a PD a foi similar à controle e 5% superior à dieta com 60% de triticale sem enzimas. A inclusão de 60% de triticale em dietas para suínos reduz a PD a . A adição de enzimas melhora a PD a em dietas com 30 e 60% de triticale.Palavras-chave: suíno, β-glucanase, fósforo, metabolizibilidade, nitrogênio, xilanase O Rio Grande do Sul é um dos estados mais importantes na produção nacional de grãos. A localização geográfica do RS apresenta condições de clima e solo favoráveis à produção ABSTRACT

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Diurnal variation in degradation of phytic acid by plant phytase in the pig stomach

Cited by 33 publications

References 28 publications

FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION

FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION

Heat-treatment, phytase and fermented liquid feeding affect the presence of inositol phosphates in ileal digesta and phosphorus digestibility in pigs fed a wheat and barley diet

Utilização do triticale e de enzimas em dietas para suínos: digestibilidade e metabolismo

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