Diuron metabolites act as endocrine disruptors and alter aggressive behavior in Nile tilapia (Oreochromis niloticus)

Boscolo, Camila Nomura Pereira; Pereira, Thiago Scremin Boscolo; Batalhão, Isabela Gertrudes; Dourado, Priscila Leocádia Rosa; Schlenk, Daniel; Almeida, Eduardo Alves de

doi:10.1016/j.chemosphere.2017.10.009

Cited by 47 publications

(27 citation statements)

References 56 publications

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“…Insecticides can cause changes that modify the endocrine response of fish (Zhang et al, 2015). These modifications may cause deregulation on the HPI axis and promote important behavioral alterations for fish (Boscolo et al, 2018), such as changes in memory consolidation (Manuel et al, 2014;Bennion et al, 2015). Memory consolidation prepares animals to defend themselves from dangerous situations, such as predation risk (Oliveira et al, 2017), via an aversive memory (Gerlai, 2016).…”

Section: Introductionmentioning

confidence: 99%

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Gusso

Reolon

Gonzalez

et al. 2020

Front. Behav. Neurosci.

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Pyriproxyfen is one of the most used larvicides and insecticides; it acts as an analog of juvenile insect hormone (a growth regulator). It is highly toxic during all stages of mosquito development, suppresses metamorphosis, and interferes in insect reproduction and proliferation. Pyriproxyfen and its main metabolite have been shown to affect brain development in rodents. This compound is employed mainly to eliminate outbreaks of the genus Aedes, even in potable water. Despite the increasing number of toxicological studies about larvicides and insecticides-with an indication of continuous use-there have been few studies about the effects of pyriproxyfen in non-target species such as fish. This study evaluated the effects of pyriproxyfen on behavioral, cognitive, and endocrine parameters in zebrafish. We exposed adult zebrafish to different pyriproxyfen (Pestanal) concentrations (0.125, 0.675, and 1.75 mg/l) for 96 h. We analyzed behavioral parameters, memory, cortisol levels, and gene expression of glucocorticoid receptor (gr) and corticotrophin-releasing factor (crf) after pyriproxyfen exposure. This exposure did not alter locomotion (distance or mean speed), anxiety-like behavior (latency to enter to the top zone of the tank or time in the top zone of the tank), and social or aggressive behavior. However, there was impaired inhibitory avoidance memory at all tested pyriproxyfen concentrations. Cortisol levels were reduced in exposed groups when compared to control or vehicle. However, gr and crf gene expression in pyriproxyfen-treated animals were unaltered when compared to control or vehicle groups. Taken together, these findings indicate that pyriproxyfen may induce cognitive impairment and altered cortisol levels in zebrafish, a non-target species.

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Section: Introductionmentioning

confidence: 99%

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Gusso

Reolon

Gonzalez

et al. 2020

Front. Behav. Neurosci.

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“…Similarly, Pereira et al (2015) observed anti-androgenic effects and delayed development of male sexual organs and germ cells in Nile tilapia exposed to diuron and diuron metabolites. Indeed, Boscolo et al (2017) observed that diuron decreased testosterone levels leading to a decrease in the aggressive behavior in Nile tilapias. Thus, the effects of these two urea-derived compounds seem to act similarly, causing anti-androgenic effects.…”

Section: Discussionmentioning

confidence: 99%

“…Diuron, another phenylurea herbicide extensively used worldwide for weed control was also found in surface waters at concentrations up to 200 ng/L in the San Francisco Bay-USA (Schlenk et al, 2012). Based on this information, studies regarding alterations in sexual hormones and gametogenesis in tilapias were previously done by our group, using 40, 100 and 200 ng/L od diuron (Pereira et al, 2015(Pereira et al, , 2016Felício et al, 2015;Boscolo et al, 2017). Therefore, tebuthiuron concentrations used in this study was chosen considering realistic tebuthiuron concentration found in natural environments but also considering other studies previously published by our laboratory with diuron, for comparative purposes.…”

Section: Experimental Designmentioning

confidence: 99%

“…Diuron, another urea-derived herbicide, was reported to cause morphological (Scheil et al, 2009;Mhadhbi and Beiras, 2012), biochemical (Sanchez-Muros et al, 2013) and physiological changes in fish (Miranda et al, 2008), besides causing also deficiency in fish growth (Nebeker and Schuytema, 1998). Diuron at concentrations similar to those commonly found in most contaminated aquatic environments (up to 200 ng/L; Schlenk et al, 2012;Köck-Schulmeyer et al, 2013;Masiá et al, 2015) have also been recently shown to alter the metabolism of sexual hormones in Nile tilapia, leading to alterations in gametogenesis and gonadosomatic index (Pereira et al, 2015(Pereira et al, , 2016Felício et al, 2015) and aggressive behavior (Boscolo et al, 2017). Schlenk et al (2012) first proposed that diuron in combination with alkylphenol ethoxylates at environmental concentrations would exert estrogenic effects by increasing vitellogenin levels in Japanese medaka (Oryzias latipes) using in vivo bioassays.…”

Section: Introductionmentioning

confidence: 99%

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Estrogenic and anti-androgenic effects of the herbicide tebuthiuron in male Nile tilapia (Oreochromis niloticus)

et al. 2018

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Tebuthiuron is a phenylurea herbicide widely used in agriculture that can reach the aquatic environments, possibly posing negative effects to the aquatic biota. Phenylurea herbicides, such as diuron, are known to cause estrogenic and anti-androgenic effects in fish, but no such effects were yet reported for tebuthiuron exposure. Thus, the aim of this study was to evaluate if tebuthiuron, at environmentally relevant concentrations (100 and 200ng/L) and after 25days of exposure have estrogenic and/or anti-androgenic effects on male of Nile tilapia (Oreochromis niloticus), through the evaluation of plasmatic testosterone (T) and estradiol (E) levels, brain aromatase (CYP19) levels (western-blot), and by evaluating the histology of the testicles. When compared to the control group, plasmatic T levels decreased about 76% in the animals exposed to 200ng/L of tebuthiuron, while E levels increased about 94%, which could be related to a significant increase (77%) in CYP19A1 levels, an enzyme that catalyzes the conversion of androgens into estrogens. Histological analyses of the testicles also demonstrated that tebuthiuron at both tested concentrations caused a decrease in the diameter of the seminiferous tubules and in the diameter of the lumen. Therefore, the gonadosomatic index (GSI) was reduced by 36% % in the animals exposed 200ng/L to tebuthiuron. Indeed, the relative frequency of spermatocytes and spermatids increased respectively 73% (200ng/L) and 61% (100ng/L) in the tebuthiuron exposed animals, possibly due to the impairment of sperm release into the lumen, that was decreased 93% (200ng/L) in the treated animals compared to the control. These results confirm that tebuthiuron causes estrogenic and anti-androgenic effects in Nile tilapias at environmentally relevant concentrations.

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“…These estrogenic activities of Diuron and its metabolites were recently studied by Pereira et al [25] and proved that the exposure of Diuron can interfere with the normal functions of the endocrine system of fish as one of the Endocrines Disrupting Chemicals (EDCs). EDCs are a class of environmental pollutants that alter the estrogenic functions of the organism through the disruption of the estrogen receptors [26]. Additionally, the major mechanisms of endocrine disruption mentioned by Carnevali et al [27] are the EDC binding to the hormone receptor as well as the inhibition or stimulation of hormone metabolism.…”

Section: Discussionmentioning

confidence: 99%

Herbicide Diuron as Endocrine Disrupting Chemicals (EDCs) through Histopathalogical Analysis in Gonads of Javanese Medaka (Oryzias javanicus, Bleeker 1854)

Kamarudin

Zulkifli

Azmai

et al. 2020

Animals

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The expeditious augmentation of the agriculture industry is leaving a significant negative impact on aquatic ecosystems. However, the awareness of the impacts of herbicide Diuron toxicities on the non-targeted aquatic organism, especially fish is still lacking. Javanese medaka, a new model fish species were exposed under sublethal levels and the long-term effects on gonads were investigated via histological studies. A total of 210 sexually mature fish were exposed to Diuron at seven different concentrations; control, solvent control, 1, 50, 100, 500, and 1000 μg/L for 21 days. In this study, Diuron caused histopathological alterations in gonads (ovary and testis) of Javanese medaka (Oryzias javanicus) by decreasing in gonadal staging and maturity of germ cells in oogenesis and spermatogenesis of female and male Javanese medaka. The results obtained in this study had proven our hypothesis that long-term exposure of herbicide Diuron can cause alterations in the gonadal histology of the adults of Javanese medaka.

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Diuron metabolites act as endocrine disruptors and alter aggressive behavior in Nile tilapia (Oreochromis niloticus)

Cited by 47 publications

References 56 publications

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Pyriproxyfen Exposure Impairs Cognitive Parameters and Alters Cortisol Levels in Zebrafish

Estrogenic and anti-androgenic effects of the herbicide tebuthiuron in male Nile tilapia (Oreochromis niloticus)

Herbicide Diuron as Endocrine Disrupting Chemicals (EDCs) through Histopathalogical Analysis in Gonads of Javanese Medaka (Oryzias javanicus, Bleeker 1854)

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