Diverse single-stranded DNA viruses associated with honey bees (Apis mellifera)

Kraberger, Simona; Cook, Chelsea N.; Schmidlin, Kara; Fontenele, Rafaela S.; Bautista, Joshua; Smith, Brian H.; Varsani, Arvind

doi:10.1016/j.meegid.2019.03.024

Cited by 35 publications

(33 citation statements)

References 38 publications

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“…Many of the newly described microviruses identified in this study group with those isolated from other arthropods such as those from honey bees [34]. Taking into consideration that only 40 blackflies were sampled, the sheer number and diversity of microviruses that have been found here is remarkable.…”

Section: Resultsmentioning

confidence: 72%

“…Genomoviruses have been frequently found to be associated with various arthropods [11,14,16,17,20,33,34] in addition to various other samples. Genomoviruses are typically ~2 kb in genome size and likely infect fungi based on a study which showed a genomovirus species, Sclerotinia sclerotiorum hypovirulence virus 1 , is able to replicate and cause hypovirulence in Sclerotinia sclerotiorum [35].…”

Section: Resultsmentioning

confidence: 99%

“…isolate ctbg173 (MH617562) [42], Pacific flying fox faeces-associated circular DNA virus-2 (KT732829) [40]. BfV-1, -2, -9, -14, -17 and -18 Reps are singletons sharing 31–42% with Reps of Apis mellifera virus 15 (MH973771) [34], Circoviridae sp. isolate ctbe41 (MH617348) [42], uncultured virus clone CG155 (KY487824) [51], CRESS virus sp.…”

Section: Resultsmentioning

confidence: 99%

“…Microviruses, which infect bacteria, are commonly found where their host is present including the microbiome of arthropods [34,54] and harbour unidirectional genomes which encode a replication initiation protein, major and minor capsid proteins and other accessory proteins [19]. Divided into two subfamilies, Gokushovirinae and Bullavirinae (International committee for virus taxonomy: 2018 release; ), microviruses are largely host specific [19].…”

Section: Resultsmentioning

confidence: 99%

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Unravelling the Single-Stranded DNA Virome of the New Zealand Blackfly

Kraberger

Schmidlin

Fontenele

et al. 2019

Viruses

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Over the last decade, arthropods have been shown to harbour a rich diversity of viruses. Through viral metagenomics a large diversity of single-stranded (ss) DNA viruses have been identified. Here we examine the ssDNA virome of the hematophagous New Zealand blackfly using viral metagenomics. Our investigation reveals a plethora of novel ssDNA viral genomes, some of which cluster in the viral families Genomoviridae (n = 9), Circoviridae (n = 1), and Microviridae (n = 108), others in putative families that, at present, remain unclassified (n = 20) and one DNA molecule that only encodes a replication associated protein. Among these novel viruses, two putative multi-component virus genomes were recovered, and these are most closely related to a Tongan flying fox faeces-associated multi-component virus. Given that the only other known multi-component circular replication-associated (Rep) protein encoding single-stranded (CRESS) DNA viruses infecting plants are in the families Geminiviridae (members of the genus Begomovirus) and Nanoviridae, it appears these are likely a new multi-component virus group which may be associated with animals. This study reiterates the diversity of ssDNA viruses in nature and in particular with the New Zealand blackflies.

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Section: Resultsmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Unravelling the Single-Stranded DNA Virome of the New Zealand Blackfly

Kraberger

Schmidlin

Fontenele

et al. 2019

Viruses

Self Cite

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“…These studies were able to expand the range of known honey bee viruses significantly and aside from numerous viruses belonging to the order Picornavirales, numerous other RNA viruses have been discovered belonging to the orders Bunyavirales, Mononegavirales (containing the family Rhabdoviridae) and Articulavirales (containing the family Orthomyxoviridae), and several unclassified RNA viruses such as LSV (33)(34)(35)(36)(37). DNA viruses have also been described, such as Apis mellifera Filamentous virus (AmFV) (38), and numerous single-stranded DNA viruses (39). While these sequencing efforts have vastly increased the number of known honey bee related viruses, the relevance of most of these viruses remains enigmatic.…”

Section: Introductionmentioning

confidence: 99%

Hymenoptera associated eukaryotic virome lacks host specificity

Deboutte

Beller

Yinda

et al. 2020

Preprint

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Recent advancements in sequencing technologies and metagenomic studies have increased the knowledge of the virosphere associated with honey bees tremendously. In this study, viral-like particle enrichment and deep sequencing was deployed to detect viral communities in managed Belgian honey bees. A substantial number of previously undescribed divergent virus genomes was detected, including a rhabdovirus and a recombinant virus possessing a divergent Lake Sinai Virus capsid and a Hepe-like polymerase. Furthermore, screening > 5,000 public sequencing datasets for the retrieved set of viral genomes revealed an additional plethora of undetected, divergent viruses present in a wide range of Hymenoptera species. The unexpected high number of shared viral genomes within the Apidae family and across different families within the order Hymenoptera suggests that many of these viruses are highly promiscuous, that virus sharing within and between Hymenoptera families occurs frequently, and that the concept of species-specific viral taxa inside the Hymenoptera should be revisited. In particular, this estimation implies that sharing of several viral species, thought to be specific for bees, across other eukaryotic taxa is rampant. This study provides important insights on the host taxonomical breadth of some of the known “bee viruses” and might have important implications on strategies to combat viruses that are relevant to pollinators.

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Honey Bee Viral Diseases

Amiri

Rueppell

2021

Honey Bee Medicine for the Veterinary Practitioner

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Diverse single-stranded DNA viruses associated with honey bees (Apis mellifera)

Cited by 35 publications

References 38 publications

Unravelling the Single-Stranded DNA Virome of the New Zealand Blackfly

Unravelling the Single-Stranded DNA Virome of the New Zealand Blackfly

Hymenoptera associated eukaryotic virome lacks host specificity

Honey Bee Viral Diseases

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