Diversity analysis of methanogens in rumen of Bubalus bubalis by 16S riboprinting and sequence analysis

Chaudhary, Prem Prashant; Sirohi, Smita; Saxena, Jyoti

doi:10.1016/j.gene.2011.11.041

Cited by 25 publications

(22 citation statements)

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“…are members of the order Methanomicrobiales that appear to be abundant in certain rumens (Yanagita et al, 2000;Tajima et al, 2001;Shin et al, 2004;Chaudhary et al, 2012), although the basis of their apparent dominance over Methanobrevibacter spp. in those cases is not known.…”

Section: Overview Of Rumen Ecology/methanogensmentioning

confidence: 99%

Section: Overview Of Rumen Ecology/methanogensmentioning

confidence: 99%

“…Methanomicrobium mobile is a hydrogenotrophic methanogen (Paynter and Hungate, 1968) that should compete directly with Methanobrevibacter spp. Other members of the order Methanomicrobiales are detected or isolated occasionally, but are never abundant (Janssen and Kirs, 2008;Kim et al, 2011;Chaudhary et al, 2012).Members of a third order, Methanosarcinales, also occur in the rumen, but are reported in much lower numbers except under some special circumstances when rumen turnover rates are very low (Rowe et al, 1979). Members of the genus Methanosarcina are capable of growth with and methane formation from hydrogen, methyl compounds and acetate.…”

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confidence: 99%

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Progress in the development of vaccines against rumen methanogens

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Janssen

Leahy

et al. 2013

Animal

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Vaccination against rumen methanogens offers a practical approach to reduce methane emissions in livestock, particularly ruminants grazing on pasture. Although successful vaccination strategies have been reported for reducing the activity of the rumen-dwelling organism Streptococcus bovis in sheep and S. bovis and Lactobacillus spp. in cattle, earlier approaches using vaccines based on whole methanogen cells to reduce methane production in sheep have produced less promising results. An anti-methanogen vaccine will need to have broad specificity against methanogens commonly found in the rumen and induce antibody in saliva resulting in delivery of sufficiently high levels of antibodies to the rumen to reduce methanogen activity. Our approach has focussed on identifying surface and membrane-associated proteins that are conserved across a range of rumen methanogens. The identification of potential vaccine antigens has been assisted by recent advances in the knowledge of rumen methanogen genomes. Methanogen surface proteins have been shown to be immunogenic in ruminants and vaccination of sheep with these proteins induced specific antibody responses in saliva and rumen contents. Current studies are directed towards identifying key candidate antigens and investigating the level and types of salivary antibodies produced in sheep and cattle vaccinated with methanogen proteins, stability of antibodies in the rumen and their impact on rumen microbial populations. In addition, there is a need to identify adjuvants that stimulate high levels of salivary antibody and are suitable for formulating with protein antigens to produce a low-cost and effective vaccine.Keywords: vaccine, antigen, antibody, methane, methanogens, Methanobrevibacter ruminantium Implications A number of different mitigation strategies are being investigated for reducing methane emissions from livestock, including the development of vaccines that specifically target the methane-producing methanogens in the rumen. Previous studies on vaccinating ruminants against the rumen-dwelling organisms Streptococcus bovis and Lactobacillus spp. have demonstrated the feasibility of vaccinating animals to produce salivary antibodies that neutralise the activity of these microbes in the rumen (Shu et al., 1999(Shu et al., , 2000a(Shu et al., , 2000b(Shu et al., and 2001Gill et al., 2000). Owing to the complexity of the rumen microbiota and diversity of methanogens in the rumen, an effective vaccine for reducing methane emissions from ruminants will need to target a wide range of methanogens in the rumen among an even more diverse assemblage of bacteria, fungi and protozoa. This paper reviews the progress towards developing an anti-methanogen vaccine. IntroductionA range of different strategies are being investigated to reduce methane emissions from farmed ruminants (reviewed by Martin et al., 2010;Buddle et al., 2011;Clark, 2013). Vaccination of ruminants against rumen methanogens has the potential to reduce methane emissions by decreasing the number or activity of meth...

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Section: Overview Of Rumen Ecology/methanogensmentioning

confidence: 99%

Section: Overview Of Rumen Ecology/methanogensmentioning

confidence: 99%

mentioning

confidence: 99%

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Progress in the development of vaccines against rumen methanogens

Wedlock

Janssen

Leahy

et al. 2013

Animal

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“…Methane is one of the most potent greenhouse gases that significantly contribute to global warming. Therefore, decreasing the number and activity of methanogens are considered as effective strategies to tackle this problem of global warming [6,9,10,57]. The most common species of methanogens isolated and identified so far from the fresh water sediment samples belong to the genera Methanosarcina, Methanomicrobium, Methanobacteriales and Methanococcus [24,26,33,35,51,53].…”

Section: Introductionmentioning

confidence: 99%

“…The most common species of methanogens isolated and identified so far from the fresh water sediment samples belong to the genera Methanosarcina, Methanomicrobium, Methanobacteriales and Methanococcus [24,26,33,35,51,53]. With the advancement of molecular techniques over the last two decades, it is now possible to study the diversity of anaerobic microorganisms in extreme environments, such as deep sediment samples and gut/rumen environments without the need of cultivation [9,10,13,29,37]. It has become evident that with a change in sediment depth, the gradient of physical and chemical conditions also changes, and provides unique environments for the growth of different types of metabolically diverse microorganisms in the sediment [34,55].…”

Section: Introductionmentioning

confidence: 99%

Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic

et al. 2014

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The variation in the diversity of methanogens in sediment depths from Sitka stream was studied by constructing a 16S rRNA gene library using methanogen-specific primers and a denaturing gradient gel electrophoresis (DGGE)-based approach. A total of nine different phylotypes from the 16S rRNA library were obtained, and all of them were clustered within the order Methanosarcinales. These nine phylotypes likely represent nine new species and at least 5-6 new genera. Similarly, DGGE analysis revealed an increase in the diversity of methanogens with an increase in sediment depth. These results suggest that Methanosarcinales phylotypes might be the dominant methanogens in the sediment from Sitka stream, and the diversity of methanogens increases as the depth increases. Results of the present study will help in making effective strategies to monitor the dominant methanogen phylotypes and methane emissions in the environment.

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Comparative community structure of archaea in rumen of buffaloes and cattle

et al. 2016

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This study indicated that there is a substantial difference in community structure of ruminal methanogens of cattle and buffaloes. The study has also indicated that the percent of species-level operational taxonomic units shared between cattle and buffalo is very low, and thus host species-specific methane mitigation strategies need to be developed for cattle and buffaloes. © 2016 Society of Chemical Industry.

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Diversity analysis of methanogens in rumen of Bubalus bubalis by 16S riboprinting and sequence analysis

Cited by 25 publications

References 30 publications

Progress in the development of vaccines against rumen methanogens

Progress in the development of vaccines against rumen methanogens

Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic

Comparative community structure of archaea in rumen of buffaloes and cattle

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