Diversity and systematics ofDeschampsia sensu lato (Poaceae), inferred from karyotypes, protein electrophoresis, total genomic DNA hybridization and chloroplast DNA analysis

García-Suárez, Rosalina; Alonso‐Blanco, Carlos; Fernandez-Carvajal, M. C.; Fernández-Prieto, Jose-Ángel; Roca, A.; Giráldez, R.

doi:10.1007/bf00982800

Cited by 19 publications

(21 citation statements)

References 17 publications

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“…Our results are in agreement with earlier reported data on the karyotypes of few Deschampsia species [1, 25–28]. This long metacentric chromosome was suggested to be a result of a translocation (or a centric fusion) between two acrocentric chromosomes, giving rise to the unusual for cereals chromosome number 2n = 26 [1]. The intercalary signal of telomere repeats in that long chromosome previously revealed by FISH [27] confirms indirectly this assumption as intercalary sites of telomere repeats are considered to be the remnants of ancestral chromosomal rearrangements occurred during the evolution [53].…”

Section: Discussionsupporting

confidence: 94%

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

et al. 2017

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The genus Deschampsia P. Beauv (Poaceae) involves a group of widespread polymorphic species. Some of them are highly tolerant to stressful and variable environmental conditions, and D. antarctica is one of the only two vascular plants growing in Antarctic. This species is a source of useful for selection traits and a valuable model for studying an environmental stress tolerance in plants. Genome diversity and comparative chromosomal phylogeny within the genus have not been studied yet as karyotypes of most Deschampsia species are poorly investigated. We firstly conducted a comparative molecular cytogenetic analysis of D. antarctica (Antarctic Peninsula) and related species from various localities (D. cespitosa, D. danthonioides, D. elongata, D. flexuosa (= Avenella flexuosa), D. parvula and D. sukatschewii by fluorescence in situ hybridization with 45S and 5S rDNA, DAPI-banding and sequential rapid in situ hybridization with genomic DNA of D. antarctica, D. cespitosa, and D. flexuosa. Based on patterns of distribution of the examined markers, chromosomes of the studied species were identified. Within these species, common features as well as species peculiarities in their karyotypic structure and chromosomal distribution of molecular cytogenetic markers were characterized. Different chromosomal rearrangements were detected in D. antarctica, D. flexuosa, D. elongata and D. sukatschewii. In karyotypes of D. antarctica, D. cespitosa, D. elongata and D. sukatschewii, 0–3 B chromosomes possessed distinct DAPI-bands were observed. Our findings suggest that the genome evolution of the genus Deschampsia involved polyploidy and also different chromosomal rearrangements. The obtained results will help clarify the relationships within the genus Deschampsia, and can be a basis for the further genetic and biotechnological studies as well as for selection of plants tolerant to extreme habitats.

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Section: Discussionsupporting

confidence: 94%

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

et al. 2017

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“…Deschampsia P. Beauv. is a cosmopolitan genus that involves a group of taxa with a wide geographical distribution [ 26 , 41 ]. The high morphological diversity makes the taxonomy of the genus very difficult [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…The D . caespitosa complex is the most studied taxon in the genus with a widespread distribution including extreme polar environments [ 41 , 42 ]. In D .…”

Section: Discussionmentioning

confidence: 99%

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Molecular Cytogenetic Analysis of Deschampsia antarctica Desv. (Poaceae), Maritime Antarctic

et al. 2015

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Deschampsia antarctica Desv. (Poaceae) (2n = 26) is one of the two vascular plants adapted to the harshest environment of the Antarctic. Although the species is a valuable model for study of environmental stress tolerance in plants, its karyotype is still poorly investigated. We firstly conducted a comprehensive molecular cytogenetic analysis of D. antarctica collected on four islands of the Maritime Antarctic. D. antarctica karyotypes were studied by Giemsa C- and DAPI/C-banding, Ag-NOR staining, multicolour fluorescence in situ hybridization with repeated DNA probes (pTa71, pTa794, telomere repeats, pSc119.2, pAs1) and the GAA simple sequence repeat probe. We also performed sequential rapid in situ hybridization with genomic DNA of D. caespitosa. Two chromosome pairs bearing transcriptionally active 45S rDNA loci and five pairs with 5S rDNA sites were detected. A weak intercalary site of telomere repeats was revealed on the largest chromosome in addition to telomere hybridization signals at terminal positions. This fact confirms indirectly the hypothesis that chromosome fusion might have been the cause of the unusual for cereals chromosome number in this species. Based on patterns of distribution of the examined molecular cytogenetic markers, all chromosomes in karyotypes were identified, and chromosome idiograms of D. antarctica were constructed. B chromosomes were found in most karyotypes of plants from Darboux Island. A mixoploid plant with mainly triploid cells bearing a Robertsonian rearrangement was detected among typical diploid specimens from Great Jalour Island. The karyotype variability found in D. antarctica is probably an expression of genome instability induced by environmental stress factors. The differences in C-banding patterns and in chromosome distribution of rDNA loci as well as homologous highly repeated DNA sequences detected between genomes of D. antarctica and its related species D. caespitosa indicate that genome reorganization involving coding and noncoding repeated DNA sequences had occurred during the divergence of these species.

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“…(Garcia-SuArez et al, 1997). From a morphological point of view they include a rather homogeneous group of Correspondence to: Professor R. Giraldez.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Avenella Drejer (Gramineae) in the Iberian Peninsula inferred from micromorphology, cytogenetic analysis, isozyme assays and in situ hybridization

García-Suárez

Alvarez

Prieto

et al. 1997

Botanical Journal of the Linnean Society

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Micromorphology, cytogenetic analysis, isozyme assays, and in situ hybridization were used in order to analyse the diversity of Avaella in the north-west Iberian Peninsula. Among the taxa analysed, somatic chromosome numbers 2n = 4x = 28 and 2n = 8x = 56 were found.Differences in habit, micromorphology and MDH patterns were also detected. Chromosome C-banding pattern and results obtained from total genomic DNA in situ hybridization indicate that the different taxa analysed are closely related. The systematic position of the different taxa is analysed.

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Diversity and systematics ofDeschampsia sensu lato (Poaceae), inferred from karyotypes, protein electrophoresis, total genomic DNA hybridization and chloroplast DNA analysis

Cited by 19 publications

References 17 publications

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

Comparative molecular cytogenetic characterization of seven Deschampsia (Poaceae) species

Molecular Cytogenetic Analysis of Deschampsia antarctica Desv. (Poaceae), Maritime Antarctic

Diversity of Avenella Drejer (Gramineae) in the Iberian Peninsula inferred from micromorphology, cytogenetic analysis, isozyme assays and in situ hybridization

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