Diversity of activator of G-protein signaling (AGS)-family proteins and their impact on asymmetric cell division across taxa

Wavreil, Florence D.M.; Yajima, Mamiko

doi:10.1016/j.ydbio.2020.07.004

Cited by 12 publications

(15 citation statements)

References 138 publications

(238 reference statements)

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“…The C. elegans GPSM2 orthologs, GPR1/2, demonstrate low sequence conservation with GPSM2 proteins despite maintaining its role in spindle orientation (Colombo et al, 2003;Srinivasan et al, 2003;Wavreil & Yajima, 2020). GPR1/2 share only an average of 23% identity with all other animals in our analyses.…”

Section: Dlg and Gpsm2 May Have Coevolved In C Elegansmentioning

confidence: 79%

“…The coevolution could be the cause or consequence of differences observed in C. elegans ACD (asymmetrical cell divisions) compared to other bilaterians. GPR1/2 and LIN-5 (NuMA) act downstream of cell polarity factors in C. elegans embryos (Colombo et al, 2003;Gotta et al, 2003;Srinivasan et al, 2003) whereas Drosophila Pins (GPSM2), Mud (NuMA), and polarity factors are mutually dependent on one another (Chia et al, 2008;Schober et al, 1999;Wavreil & Yajima, 2020;Yu et al, 2000). Furthermore, C. elegans use an evidently unique system that relies on the nematode-specific protein LET-99 to localize GPR1/2 (Krueger et al, 2010;Tsou et al, 2002).…”

Section: Dlg and Gpsm2 May Have Coevolved In C Elegansmentioning

confidence: 99%

“…GPSM2 is thought to work as an adaptor; it binds to NuMA through its Nterminal TPRs (tetratricopeptide repeats) and to myristoylated Gαi through its C-terminal GoLoco domains, thereby anchoring the complex to the cell cortex (Figure 1). This structure is conserved across taxa, though the number of TPR and GoLoco domains varies between and within phyla (Wavreil & Yajima, 2020) A notable exception is the nematode worm, in which two functional homologs to GPSM2, called GPR1 and 2, have a unique structure that lacks GoLoco domains and may contain one or two TPRs, though this is debated (Nguyen-Ngoc et al, 2007;Siller & Doe, 2009;Willard et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Interaction between Discs large and GPSM2: A Comparison Across Species

Schiller

Bergstralh

2021

Preprint

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The orientation of the mitotic spindle determines the direction of cell division, and therefore contributes to tissue shape and cell fate. Interaction between the multifunctional scaffolding protein Discs large (Dlg) and the canonical spindle orienting factor GPSM2 (also called Pins in Drosophila and LGN in vertebrates) has been established in bilaterian models, but its function remains unclear. We used a phylogenetic approach to test whether the interaction is obligate in animals, and in particular whether GPSM2 evolved in multicellular organisms as a Dlg-binding protein. We show that Dlg diverged in C. elegans and the syncytial sponge O. minuta and propose that this divergence may correspond to differences in spindle orientation requirements between these organisms and the canonical pathways described in bilaterians. We also demonstrate that GPSM2 is present in basal animals, but the established Dlg-interaction site cannot be found in either Placozoa or Porifera. Our results suggest that the interaction between GPSM2 and Dlg appeared in Cnidaria, and we therefore speculate that it may have evolved to promote accurate division orientation in the nervous system. This work reveals the evolutionary history of the GPSM2/Dlg interaction and suggests new possibilities for its importance in spindle orientation during epithelial and neural tissue development.

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Section: Dlg and Gpsm2 May Have Coevolved In C Elegansmentioning

confidence: 79%

Section: Dlg and Gpsm2 May Have Coevolved In C Elegansmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interaction between Discs large and GPSM2: A Comparison Across Species

Schiller

Bergstralh

2021

Preprint

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“…The C. elegans Pins/LGN/GPSM2 orthologs, GPR1/2, demonstrate low sequence conservation with Pins/LGN/GPSM2 proteins despite maintaining its role in spindle orientation ( Colombo et al, 2003 ; Srinivasan et al, 2003 ; Wavreil and Yajima, 2020 ). GPR1/2 share only an average of 23% identity with all other animals in our analyses.…”

Section: Discussionmentioning

confidence: 99%

“…1 ). This structure is conserved across taxa, though the number of TPR and GoLoco domains varies between and within phyla ( Wavreil and Yajima, 2020 ). A notable exception is the nematode worm, in which two functional homologs to Pins/LGN/GPSM2, called GPR1 and 2, have a unique structure that lack GoLoco domains and may contain one or two TPRs, though this is debated ( Nguyen-Ngoc et al, 2007 ; Siller and Doe, 2009 ; Willard et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Interaction between Discs large and Pins/LGN/GPSM2: a comparison across species

Schiller

Bergstralh

2021

Biology Open

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The orientation of the mitotic spindle determines the direction of cell division, and therefore contributes to tissue shape and cell fate. Interaction between the multifunctional scaffolding protein Discs large (Dlg) and the canonical spindle orienting factor GPSM2 (called Pins in Drosophila and LGN in vertebrates) has been established in bilaterian models, but its function remains unclear. We used a phylogenetic approach to test whether the interaction is obligate in animals, and in particular whether Pins/LGN/GPSM2 evolved in multicellular organisms as a Dlg-binding protein. We show that Dlg diverged in C. elegans and the syncytial sponge O. minuta and propose that this divergence may correspond to differences in spindle orientation requirements between these organisms and the canonical pathways described in bilaterians. We also demonstrate that Pins/LGN/GPSM2 is present in basal animals, but the established Dlg-interaction site cannot be found in either Placozoa or Porifera. Our results suggest that the interaction between Pins/LGN/GPSM2 and Dlg appeared in Cnidaria, and we therefore speculate that it may have evolved to promote accurate division orientation in the nervous system. This work reveals the evolutionary history of the Pins/LGN/GPSM2-Dlg interaction and suggests new possibilities for its importance in spindle orientation during epithelial and neural tissue development.

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Micromere formation and its evolutionary implications in the sea urchin

Emura

Yajima

2022

Current Topics in Developmental Biology

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Diversity of activator of G-protein signaling (AGS)-family proteins and their impact on asymmetric cell division across taxa

Cited by 12 publications

References 138 publications

Interaction between Discs large and GPSM2: A Comparison Across Species

Interaction between Discs large and GPSM2: A Comparison Across Species

Interaction between Discs large and Pins/LGN/GPSM2: a comparison across species

Micromere formation and its evolutionary implications in the sea urchin

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