Diversity of Adenostemma lavenia, multi-potential herbs, and its kaurenoic acid composition between Japan and Taiwan

Maeda, Miwa; Suzuki, Mayu; Fuchino, Hiroyuki; Tanaka, Nagisa; Kobayashi, Takahiro; Isogai, Ryosuke; Batubara, Irmanida; Iswantini, Dyah; Matsuno, Michiyo; Kawahara, Nobuo; Koketsu, Mamoru; Hamamoto, Akie; Takemori, Hiroshi

doi:10.1007/s11418-021-01565-3

Cited by 9 publications

(4 citation statements)

References 35 publications

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“…showed various activities such as antibacterial, antiplatelet aggregation, and anti‐inflammatory [23–24] . On the other hand, ent‐16α,17‐hydroxy‐19‐kauranoic acid and 9,16‐dioxyhydroxy‐10,12,14‐triene‐18‐carboxylic acid reported to have antioxidant, anti‐inflammatory, and antimicrobials properties [25–27] . These results suggested that these dominant compounds inside F4 fraction proven with good antibacterial, antioxidant as well as antidiabetic activities might affected by the presence of some potential compounds, primarily flavonoid and phenolic class.…”

Section: Resultsmentioning

confidence: 97%

Investigations of Antibacterial, Antioxidant, and Antidiabetic Potential of Extract and Its Active Fractions from the Leaves of Horsfieldia spicata (Roxb.) J. Sinclair

Prastya¹,

Ariani²,

Darmawan³

et al. 2023

Chemistry & Biodiversity

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This study was undertaken to analyse the potential bioactivities including antibacterial, antioxidant and antidiabetic derived from the methanolic extract and the column chromatography ethyl acetate fraction (AcOEt Fr) of Horsfieldia spicata leaves. Methanolic extract and 4 other fractions was calculated for total phenol and flavonoid contents along with tested for antibacterial, antioxidant and antidiabetic properties. Interestingly, the AcOEt Fr had the highest value for total flavonoid content and the best antioxidant, and antidiabetic activities. Therefore, the AcOEt Fr was further separated using column chromatography technique for obtaining 9 selected fractions namely fraction 1 (F1) ‐ fraction 9 (F9) which were further tested. The results showed that the AcOEt column chromatography fractions namely F2, F3, F4 and F6 had the best clear inhibition antibacterial value against all bacterial tested. In addition, these fractions also exhibited better Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) values than others. Antioxidant, 2,2‐diphenylpicrylhydrazyl (DPPH) assayed indicated that AcOEt Fr had the strongest IC50 value of 47.30 μg/mL. Further, F4 column chromatography fraction showed the best inhibition against α‐Glucosidase enzyme related to antidiabetic activity with an IC50 value of 6.11 μg/mL. Liquid chromatography tandem‐mass spectrometry (LC/MS/MS) analysis identified that F4 derived from AcOEt fraction had several compounds belonging to the flavonoid and phenolics such as 3′,5‐dihydroxy‐7,4′‐dimethoxyflavone, 5,7‐dihydroxy‐3‐(4′‐hydroxybenzyl)chromone, and Kadsurenin I.

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Section: Resultsmentioning

confidence: 97%

Investigations of Antibacterial, Antioxidant, and Antidiabetic Potential of Extract and Its Active Fractions from the Leaves of Horsfieldia spicata (Roxb.) J. Sinclair

Prastya¹,

Ariani²,

Darmawan³

et al. 2023

Chemistry & Biodiversity

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“…In this current study, A. madurense had the lowest TPC. According to (Maeda et al 2022), A. madurense contains terpenoids, specifically kaurenoic acid, which possesses anti-inflammatory, antimelanogenic, and antioxidant properties. On the other hand, A. platyphyllum has a higher TPC than total flavonoid content (TFC).…”

Section: Discussionmentioning

confidence: 99%

“…Because A. lavenia leaves contain various secondary metabolites such as terpenoids, flavonoids, alkaloids, polyphenols, and essential oils, they are frequently utilized (Batubara and Prastya 2020b). Different species of Adenostemma, which have morphological traits similar to A. lavenia, such as Adenostemma madurense and Adenostemma platyphyllum, have been found to possess bioactive chemicals with antibacterial, anti-inflammatory, and antioxidant properties (Prasad et al 2013;Maeda et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Variation of morphological characteristics, total phenolic, and total flavonoid in Adenostemma lavenia, A. madurense, and A. platyphyllum

NURLELA

NURFALAH²,

ANANDA³

et al. 2022

Biodiversitas

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Abstract. Nurlela, Nurfalah R, Ananda F, Ridwan T, Ilmiawati A, Nurcholis W, Takemori H, Batubara I. 2022. Variation of morphological characteristics, total phenolic, and total flavonoid in Adenostemma lavenia, A. madurense, and A. platyphyllum. Biodiversitas 23: 3999-4005. Adenostemma has long been used as a herbal medication. Despite some studies regarding the morphology and potency of Adenostemma carried out, the variation of morphological traits, total phenolic content (TPC), and total flavonoid content (TFC) on different species of Adenostemma has been poorly investigated. This study was designed to observe the morphological characteristics, TPC, and TFC of Adenostemma lavenia, A. madurense, and A. platyphyllum. Morphological characteristics were observed using qualitative and quantitative parameters. The colorimetric method determined TPC and TFC in plant leaf ethanol extracts. The plants were morphologically diverse, which significant variation among the three species in plant height, length and width of leaf, and internodes distance. Morphological traits such as leaf length and width, plant height, and internodes distance significantly correlate with phenolic and flavonoid content in Adenostemma. These may be used as important selection criteria in Adenostemma for obtaining high phenolic and flavonoid content. The study revealed that different species of these plants with varying morphological traits substantially impact the content of their chemical compounds. The highest TPC and TFC were found in A. lavenia, suggesting that it is potentially an alternative source of bioactive compounds as herbal medicine.

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“…The activation of NF-E2-related factor 2 (NRF2) is a mechanism of 11αOH-KA-mediated suppression of TYR gene expression [ 29 ]. However, NRF2 has been reported to suppress MITF gene expression [ 30 , 31 ], leading to the downregulation of TYR and TYRP-1. There evidences suggest that in addition to NRF2, an unknown factor associated with MITF in the promoter may contribute to TYR-specific transcription, which may be also the case for GIF-2209.…”

Section: Discussionmentioning

confidence: 99%

GIF-2209, an Oxindole Derivative, Accelerates Melanogenesis and Melanosome Secretion via the Modification of Lysosomes in B16F10 Mouse Melanoma Cells

et al. 2021

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Melanogenesis and melanosome secretion are regulated by several mechanisms. In this study, we found that the oxindole derivative GIF-2209 accelerated melanogenesis associated with the discrimination in the expression and intracellular distributions of two melanogenic enzymes, tyrosinase (TYR) and tyrosinase-related protein-1 (TYRP-1). GIF-2209 upregulated the expression of TYR via a microphthalmia transcription factor (MITF)-independent mechanism, leading to high expression of protein. In contrast, GIF-2209 did not alter the mRNA levels of TYRP-1 and suppressed its protein levels. GIF-2209 induced the dissociation of TYR from TYRP-1 but did not alter the association between TYR and CD63, a melanosome and lysosome marker. The protein levels of CD63 were also upregulated by GIF-2209. GIF-2209 induced lysosome expansion and redistribution in all areas of the cytosol, accompanied by autophagy acceleration (upregulation of LC3BII protein levels and downregulation of p62 protein levels). In addition, GIF-2209 stimulated the secretion of melanosomes containing high levels of TYR, TYRP-1, and CD63 proteins. The GIF-2209 mediated melanosome secretion was sensitive to the lysosome inhibitor chloroquine. These results suggest that GIF-2209 may activate lysosomal functions with TYR gene expression, while it accelerates melanosome secretion, which finally leads to the depletion of intracellular melanogenic enzyme, especially TYRP-1 protein.

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Diversity of Adenostemma lavenia, multi-potential herbs, and its kaurenoic acid composition between Japan and Taiwan

Cited by 9 publications

References 35 publications

Investigations of Antibacterial, Antioxidant, and Antidiabetic Potential of Extract and Its Active Fractions from the Leaves of Horsfieldia spicata (Roxb.) J. Sinclair

Investigations of Antibacterial, Antioxidant, and Antidiabetic Potential of Extract and Its Active Fractions from the Leaves of Horsfieldia spicata (Roxb.) J. Sinclair

Variation of morphological characteristics, total phenolic, and total flavonoid in Adenostemma lavenia, A. madurense, and A. platyphyllum

GIF-2209, an Oxindole Derivative, Accelerates Melanogenesis and Melanosome Secretion via the Modification of Lysosomes in B16F10 Mouse Melanoma Cells

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