Divisome and segrosome components of Deinococcus radiodurans interact through cell division regulatory proteins

Maurya, Ganesh Kumar; Modi, Kruti; Misra, Hari S.

doi:10.1099/mic.0.000330

Cited by 28 publications

(27 citation statements)

References 58 publications

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“…Interactions between DivIVA and ParB were also detected in the non-sporulating bacteria S. pneumoniae [169] and D. radiodurans [180,181]. In the latter, DivIVA interacts with various ParBs encoded by the multipartite genome, with ParB1 (chrI), ParB2 (chrII), and ParB3 and ParB4 from the megaplasmid and plasmid, respectively.…”

Section: The Interactions Of Parb With Topological Determinants Durinmentioning

confidence: 93%

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

et al. 2020

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The segregation of newly replicated chromosomes in bacterial cells is a highly coordinated spatiotemporal process. In the majority of bacterial species, a tripartite ParAB-parS system, composed of an ATPase (ParA), a DNA-binding protein (ParB), and its target(s) parS sequence(s), facilitates the initial steps of chromosome partitioning. ParB nucleates around parS(s) located in the vicinity of newly replicated oriCs to form large nucleoprotein complexes, which are subsequently relocated by ParA to distal cellular compartments. In this review, we describe the role of ParB in various processes within bacterial cells, pointing out interspecies differences. We outline recent progress in understanding the ParB nucleoprotein complex formation and its role in DNA segregation, including ori positioning and anchoring, DNA condensation, and loading of the structural maintenance of chromosome (SMC) proteins. The auxiliary roles of ParBs in the control of chromosome replication initiation and cell division, as well as the regulation of gene expression, are discussed. Moreover, we catalog ParB interacting proteins. Overall, this work highlights how different bacterial species adapt the DNA partitioning ParAB-parS system to meet their specific requirements.

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Section: The Interactions Of Parb With Topological Determinants Durinmentioning

confidence: 93%

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

et al. 2020

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“…In such a scenario, if DivIVA senses the geometry of the cells, marking negative curvature at the poles for MinC to oscillate in parallel to the central septum in this bacterium, cannot be ruled out. Additionally, it has been demonstrated that DivIVA interact with proteins involved in genome maintenance and those regulates cell division(Maurya et al, 2016, Chaudhary et al, 2019. These anticipated morphological changes in D. radiodurans during cell division, and drDivIVA interaction with macromolecular complexes associated with cell division and genome segregation together suggest the possible mechanisms by which drDivIVA could help in cell polarity determination in this bacterium.…”

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confidence: 89%

DivIVA is essential inDeinococcus radioduransand its C terminal domain regulates new septum orientation during cell division

Chaudhary

Kota

Misra

2020

Preprint

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FtsZ assembly at mid cell position in rod shaped bacteria is regulated by gradient of MinCDE complex across the poles. In round shaped bacteria, which lack predefined poles and the next plane of cell division is perpendicular to previous plane, the determination of site for FtsZ assembly is intriguing. Deinococcus radiodurans a coccus shaped bacterium, is characterized for its extraordinary resistance to DNA damage. Here we report that DivIVA a putative component of Min system in this bacterium (drDivIVA) interacts with cognate cell division and genome segregation proteins. The deletion of full length drDivIVA was found to be indispensable while its C-terminal deletion (△divIVAC) was dispensable but produced distinguishable phenotypes like slow growth, altered plane for new septum formation and angular septum. Both wild type and mutant showed FtsZ foci formation and their gamma radiation responses were nearly identical. But unlike in wild type, the FtsZ localization in mutant cells was found to be away from orthogonal axis with respect to plane of previous septum. Notably, DivIVA-RFP localizes to membrane during cell division and then perpendicular to previous plane of cell division. In trans expression of drDivIVA in △divIVAC background could restore the wild type pattern of septum formation perpendicular to previous septum. These results suggested that DivIVA is an essential protein in D. radiodurans and the C-terminal domain that contributes to its interaction with MinC determines the plane of new septum formation, possibly by controlling MinC oscillation through orthogonal axis in the cells.

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“…Cells co-transformed with empty vectors pUT18 and pKNT25 193 were used as negative controls and those transformed with plasmids pKNTEFZ (E. coli FtsZ) 194 and pUTEFA (E .coli FtsA) were used as positive controls. The co-transformants obtained 195 were grown in liquid LB broth containing kanamycin (50 µg/ml), ampicillin (100 µg/ml) and 196 IPTG (0.5 mM) for 24-36h at 30°C and β-galactosidase enzyme activity was assayed and 197 calculated as described earlier in (Maurya et. al., 2016;Battesti and Bouveret, 2012) and 198 plotted using GraphPad Prizm5.…”

Section: In Recombinant E Coli Hostmentioning

confidence: 99%

Topoisomerase IB interacts with genome segregation proteins and is involved in multipartite genome maintenance inDeinococcus radiodurans

Kota¹,

Chaudhary

Mishra

et al. 2020

Preprint

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31Deinococcus radiodurans, an extremophile, resistant to many abiotic stresses including 32 ionizing radiation, has 2 type I topoisomerases (drTopo IA and drTopo IB) and one type II 33 topoisomerase (DNA gyrase). The role of drTopo IB in guanine quadruplex DNA (G4 DNA) 34 metabolism was shown in vitro. Here we report that D. radiodurans cells lacking drTopo IB 35 (∆topoIB) show sensitivity to G4 DNA binding drug (NMM) under normal growth conditions. 36 The activity of G4 motif containing promoters like mutL and recQ was reduced in the 37 presence of NMM in mutant cells. In mutant cells, the percentage of anucleated cells was 38 more while the ploidy numbers of genome elements were less as compared to wild type. 39 Protein-protein interaction studies showed that drTopo IB interacts with genome segregation 40 and DNA replication initiation (DnaA) proteins. The typical patterns of cellular localization of 41 GFP-PprA were affected in the mutant cells. Microscopic examination of D. radiodurans cells 42 expressing drTopo IB-RFP showed its localization on nucleoid forming a streak parallel to the 43 old division septum and perpendicular to newly formed septum. These results together suggest 44 the role of drTopo IB in genome maintenance in this bacterium. 45 46 Radioresistance; Topoisomerases. 48 49 50 51 52 53 54 55 56 57 58 DNA transaction processes like unwinding, rewinding, movement of proteins on DNA, the 60 formation of higher-order structures during DNA compaction leads to topological 61 entanglements. These topological barriers if unresolved, pose threats to genome stability. 62 DNA topoisomerases are ubiquitous, found in all organisms which resolve the topological 63 constraints in the DNA. Based on the structure and kind of action topoisomerases are 64 classified into type I and type II enzymes. Unlike type II DNA topoisomerases that cleave 65 both the DNA strands and change the linking number in the multiples of two, the type I 66 enzyme reversibly cleaves one strand at a time and the change the linking number in the 67 increment of one. Type I topoisomerases are subdivided into type IA and type IB subfamilies.68 Type IA topoisomerases cleave the DNA strand and make a 5'-phosphotyrosine covenant 69 intermediate with a free 3'-OH strand, while type IB topoisomerases prefer double-stranded 70 DNA, forms a 3'-phosphotyrosine covenant intermediate with a free 5'-OH strand. Type IA 71 enzymes relax only negatively supercoiled DNA, while type IB enzymes relax both negative 72 and positive supercoiled DNA. Both the type I enzymes conserves the energy of the 73 phosphodiester bond in the protein -DNA covalent intermediate, thus overall cleavage and re-74 ligation reactions with DNA strand do not require ATP. Type II topoisomerases are also sub 75 divided into type IIA and type IIB, where type IIA enzymes are present in all domains of life 76 while type IIB enzymes are present in archaea and in some higher plants (Champoux, 2001; 77 Chen et al., 2013). Escherichia coli contains 4 topoisomerases, 2 type I topoisomeras...

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Divisome and segrosome components of Deinococcus radiodurans interact through cell division regulatory proteins

Cited by 28 publications

References 58 publications

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes

DivIVA is essential inDeinococcus radioduransand its C terminal domain regulates new septum orientation during cell division

Topoisomerase IB interacts with genome segregation proteins and is involved in multipartite genome maintenance inDeinococcus radiodurans

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