DMSO Represses Inflammatory Cytokine Production from Human Blood Cells and Reduces Autoimmune Arthritis

Elisia, Ingrid; Nakamura, Hisae; Lam, Vivian; Hofs, Elyse; Cederberg, Rachel A.; Cait, Jessica; Hughes, Michael R.; Lee, Leora; Jia, William; Adomat, Hans; Guns, Emma S. Tomlinson; McNagny, Kelly M.; Samudio, Ismael; Krystal, Gerald

doi:10.1371/journal.pone.0152538

Cited by 76 publications

(57 citation statements)

References 47 publications

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“…DMSO solvent was used to dissolve the propofol powder, and then diluted to ensure that the concentration of DMSO would not damage the cells and affect our experimental results. It was reported that DMSO could alleviate the damage of ROS to cells [30]. However, DMSO, at the concentration needed to dissolve propofol as used in our experiment did not demonstrate the above mentioned beneficial effects, indicating that the beneficial effects observed are solely the effects of propofol.…”

Section: Discussioncontrasting

confidence: 60%

Propofol Through Upregulating Caveolin-3 Attenuates Post-Hypoxic Mitochondrial Damage and Cell Death in H9C2 Cardiomyocytes During Hyperglycemia

Deng

Wang

Zhang

et al. 2017

Cell Physiol Biochem

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Background/Aims: Hearts from diabetic subjects are susceptible to myocardial ischemia reperfusion (I/R) injury. Propofol has been shown to protect against myocardial I/R injury due to its antioxidant properties while the underlying mechanism remained incompletely understood. Thus, this study aimed to determine whether or not propofol could attenuate myocardial I/R injury by attenuating mitochondrial dysfunction/damage through upregulating Caveolin (Cav)-3 under hyperglycemia. Methods: Cultured rat cardiomyocyte H9C2 cells were subjected to hypoxia/reoxygenation (H/R) in the absence or presence of propofol under high glucose (HG), and cell viability, lactate dehydrogenase (LDH) and mitochondrial viability as well as creatine kinase-MB (CK-MB), cardiac troponin I (cTnI) and intracellular adenosine triphosphate (ATP) content were measured with colorimetric Enzyme-Linked Immunosorbent Assays. Intracellular levels of oxidative stress was assessed using 2,7-dichlorodihydrofluorescein diacetate (DCF-DA) fluorescent staining and mitochondrial-dependent apoptosis was assessed by detecting mitochondrial membrane potential and the activation of apoptotic caspases 3 and 9. Results: Exposure of cells to HG without or with H/R both significantly increased cell injury, cell apoptosis and enhanced oxidative stress that were associated with mitochondrial dysfunction and decreased Cav-3 protein expression. All these changes were further exacerbated following H/R under HG. Administration of propofol at concentrations from 12.5 to 50 µM but not 100 µM significantly attenuated H/R injury that was associated with increased Cav-3 expression and activation of the prosurvival proteins Akt and STAT3 with the optimal protective effects seen at 50 µM of propofol (P25). The beneficial effects of propofol(P25) were abrogated by Cav-3 disruption with β-methyl-cyclodextrin. Conclusion: Propofol counteracts cardiomyocyte H/R injury by attenuating mitochondrial damage and improving mitochondrial biogenesis through upregulating Cav-3 during hyperglycemia.

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Section: Discussioncontrasting

confidence: 60%

Propofol Through Upregulating Caveolin-3 Attenuates Post-Hypoxic Mitochondrial Damage and Cell Death in H9C2 Cardiomyocytes During Hyperglycemia

Deng

Wang

Zhang

et al. 2017

Cell Physiol Biochem

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“…Another study also reported the reduction of ERK phosphorylation, a kinase upstream of mTOR [31]. The effects observed here with 0.1% DMSO may indicate a proinflammatory response [101][102][103] with subsequent activation on mTOR [104,105].…”

Section: Discussionmentioning

confidence: 51%

Cannabidiol modulates phosphorylated rpS6 signalling in a zebrafish model of Tuberous Sclerosis Complex

Serra

Scheldeman

Bazelot

et al. 2019

Behavioural Brain Research

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Tuberous sclerosis complex (TSC) is a rare disease caused by mutations in the TSC1 or TSC2 genes and is characterized by widespread tumour growth, intractable epilepsy, cognitive deficits and autistic behaviour. CBD has been reported to decrease seizures and inhibit tumour cell progression, therefore we sought to determine the influence of CBD on TSC pathology in zebrafish carrying a nonsense mutation in the tsc2 gene. CBD treatment from 6 to 7 days post-fertilization (dpf) induced significant anxiolytic actions without causing sedation. Furthermore, CBD treatment from 3 dpf had no impact on tsc2-/larvae motility nor their survival. CBD treatment did, however, reduce the number of phosphorylated rpS6 positive cells, and their cross-sectional cell size. This suggests a CBD mediated suppression of mechanistic target of rapamycin (mTOR) activity in the tsc2-/larval brain.

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“…Indeed, oily formulations are not compatible with hydrophilic cell culture medium unless they are solubilized in organic solvents. The problem is that organic solvents can modify and alter the cellular response (Blachley et al, 1985;Luo et al, 1999;Elisia et al, 2016). In light of this, we developed a method that doesn't require any organic solvent.…”

Section: Discussionmentioning

confidence: 99%

Oily formulations challenge: how to evaluate their beneficial effects in hydrophilic cell-based models?

Olivier

L’Hermitte²,

Rat

et al. 2018

OCL

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In the European Union, Israel and India, testing cosmetic products or their ingredients on animals is prohibited. In this context, in vitro cell models play a pivotal role in the evaluation of both safety and beneficial effects of cosmetics. Oily formulations, widely used in cosmetics, are complex to study on cell models due to their lipophilic nature that doesn’t match with hydrophilic culture medium. Organic solvents are then required to solubilize oily formulations, but they can interfere with the cellular response. To avoid the use of organic solvents, we developed a method based on cells to evaluate potential beneficial effects of oily formulations. Our method, suitable for high throughput screening, consists in: (1) incubating cells with oily formulations for a short time followed by a recovery period in culture medium and (2) studying cell parameters using robust techniques such as cytofluorometry and fluorescence resonance energy transfer (FRET). Depending on the studied cell parameter, various beneficial effects can be revealed like antioxidant, anti-inflammatory and skin regeneration. The field of cell parameters is open and can be extended to new perspectives in the development of oily formulations.

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DMSO Represses Inflammatory Cytokine Production from Human Blood Cells and Reduces Autoimmune Arthritis

Cited by 76 publications

References 47 publications

Propofol Through Upregulating Caveolin-3 Attenuates Post-Hypoxic Mitochondrial Damage and Cell Death in H9C2 Cardiomyocytes During Hyperglycemia

Propofol Through Upregulating Caveolin-3 Attenuates Post-Hypoxic Mitochondrial Damage and Cell Death in H9C2 Cardiomyocytes During Hyperglycemia

Cannabidiol modulates phosphorylated rpS6 signalling in a zebrafish model of Tuberous Sclerosis Complex

Oily formulations challenge: how to evaluate their beneficial effects in hydrophilic cell-based models?

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