DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region

Guo, Yahong; Kondo, Kenji; Terabayashi, Susumu; Yamamoto, Yutaka; Shimada, Hiroshi; Fujita, Mitsue; Kawasaki, Takeshi; Maruyama, Toru; Goda, Yukihiro; Mizukami, Hajime

doi:10.1007/s11418-006-0032-8

Cited by 11 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As references, we used leaves of A. lancea, A. chinensis, and A. japonica that were botanically TCT GGT TGG AAT GGT GAC AAC ATG C Forward 335 CCA GGA AGA GCT TCA CTC AAA GCT T Reverse authenticated. The ITS1 sequence between the 18S-5.8S rRNA genes [2,3] derived from the Sojutsu samples was compared with those obtained from the leaves. The 167-base-pair (bp) ITS1 sequence from five pieces of the Sojutsu sample was identical to those of A. chinensis leaf, i.e., 100% homology, whereas it was 99.4% (166 bp/167 bp) and 97.0% (162 bp/167 bp) identical to those of the A. lancea and A. japonica leaves, respectively.…”

Section: Identification Of Atractylodes Species In a Sojutsu Samplementioning

confidence: 99%

“…These species are classified by the Japanese Pharmacopoeia into two crude drugs, Sojutsu and Byakujutsu [1]; Sojutsu ("Atractylodes Lancea Rhizome") is defined as the rhizome of Atractylodes lancea De Candolle, Atractylodes chinensis Koidzumi, or their interspecific hybrids (Asteraceae), whereas Byakujutsu ("Atractylodes Rhizome") is defined as the rhizome of Atractylodes japonica Koidzumi ex Kitamura or Atractylodes macrocephala Koidzumi (= Atractylodes ovata De Candolle). The Atractylodes species of Sojutsu samples can be discriminated by the nucleotide sequence of internal transcribed spacer 1 (ITS1) located between the 18S rRNA and 5.8S rRNA genes [2,3]. Sojutsu contains sesquiterpenoids (e.g., β-eudesmol, hinesol, β-selinene, elemol, and α-bisabolol) and polyacetylene compounds (e.g., atractylodin and atractylodinol) [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antiinflammatory constituents of Atractylodes chinensis rhizome improve glomerular lesions in immunoglobulin A nephropathy model mice

et al. 2019

Self Cite

View full text Add to dashboard Cite

The crude drug Sojutsu, as defined by the Japanese Pharmacopoeia, is the rhizome of Atractylodes lancea De Candolle, Atractylodes chinensis Koidzumi, or their interspecific hybrids (Asteraceae). Sojutsu is one of the traditional Kampo formulas, which are administered to patients suffering from stomach disorders, edema, and nephrotic syndrome. Although antiinflammatory effects of Sojutsu have been reported, its effects on the liver and kidney have not been extensively investigated. Here, we used a Sojutsu sample identified as A. chinensis rhizome and isolated several constituents from its ethyl acetate (EtOAc)-soluble fraction that decreased production of the proinflammatory mediator nitric oxide (NO) in interleukin 1β-treated rat hepatocytes. Among the constituents in this fraction, atractylodin showed the highest activity to suppress NO production, whereas hinesol, β-eudesmol, and α-bisabolol showed low activity. Atractylodin decreased the levels of inducible nitric oxide synthase, tumor necrosis factor α, and lipocalin 2 messenger RNAs (mRNAs). The EtOAc-soluble fraction of the A. chinensis rhizome extract was administered daily for 20 weeks to high immunoglobulin A (HIGA) mice, whose pathological findings resemble human immunoglobulin A nephropathy. This fraction decreased the weight of white adipose tissue and decreased mesangial proliferation and immunoglobulin A deposition in glomeruli. These results indicate that the EtOAc-soluble fraction, which included antiinflammatory constituents, may be responsible for improvement of the mesangial lesions in HIGA mice.

show abstract

Section: Identification Of Atractylodes Species In a Sojutsu Samplementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiinflammatory constituents of Atractylodes chinensis rhizome improve glomerular lesions in immunoglobulin A nephropathy model mice

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Crude drug samples (Table 1) were purchased in local markets in China, and voucher samples were deposited in the Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Nagoya City University. The origins of the crude drug samples were confirmed by the DNA authentication method as described previously [10].…”

Section: Crude Drugsmentioning

confidence: 99%

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

et al. 2010

Self Cite

View full text Add to dashboard Cite

(1)H-NMR spectroscopy was successfully applied to the quantitative determination of atractylon in Atractylodis Rhizoma (dried rhizomes of Atractylodes ovata and A. japonica) and Atractylodis Lanceae Rhizoma (dried rhizomes of Atractylodes lancea and A. chinensis). The analysis was carried out by comparing the integral of the H-12 singlet signal of atractylon, which was well separated in the range of delta 6.95-7.05 ppm in the NMR spectrum, with the integral of a hexamethyldisilane (HMD) signal at delta 0 ppm. The atractylon contents obtained by the (1)H-NMR spectroscopy were consistent with those obtained by the conventional HPLC analysis. The present method requires neither reference compounds for calibration curves nor sample pre-purification. It also allows simultaneous determination of multiple constituents in a crude extract. Thus, it is applicable to chemical evaluation of crude drugs as a powerful alternative to various chromatographic methods.

show abstract

“…The ITS sequences are highly conserved throughout plant species but are suitable markers for the interspecies variation and intraspecies conservation. Thus, ITS sequences are suitable targets for investigations of plant phylogenetic relationships within the same genus and species [15][16][17][18]. The phylogenetic relationship of the Panax genus has been more recently studies and so the relationship of any new strains found within Panax could be better positioned.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Korean Mountain Ginseng Using an Amplification Refractory Mutation System (ARMS)

In¹,

Kim²,

Lee³

et al. 2010

Journal of Ginseng Research

View full text Add to dashboard Cite

: Expensive herbs such as ginseng are always a possible target for fraudulent labeling. New mountain ginseng strains have occasionally been found deep within mountain areas and commercially traded at exorbitant prices. However, until now, no scientific basis has existed to distinguish such ginseng from commonly cultivated ginseng species other than by virtue of being found within deep mountain areas. Polymerase chain reaction (PCR) analysis of the internal transcribed spacer has been shown to be an appropriate method for the identification of the most popular species (Panax ginseng) in the Panax ginseng genus. A single nucleotide polymorphism (SNP) has been identified between three newly found mountain ginseng (KGD4, KGD5, and KW1) and already established Panax species. Specific PCR primers were designed from this SNP site within the sequence data and used to detect the mountain ginseng strains via multiplex PCR. The established multiplex-PCR method for the simultaneous detection of newly found mountain ginseng strains, Korean ginseng, and foreign ginseng in a single reaction was determined to be effective. This study is the first report of scientific discrimination of "mountain ginsengs" and describes an effective method of identification for fraud prevention and for uncovering the possible presence of other, cheaper ginseng species on the market.

show abstract

DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region

Abstract: The internal transcribed spacer 1 (ITS1) region between 18S and 5.

Cited by 11 publications

References 8 publications

Antiinflammatory constituents of Atractylodes chinensis rhizome improve glomerular lesions in immunoglobulin A nephropathy model mice

Antiinflammatory constituents of Atractylodes chinensis rhizome improve glomerular lesions in immunoglobulin A nephropathy model mice

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

Molecular Identification of Korean Mountain Ginseng Using an Amplification Refractory Mutation System (ARMS)

Contact Info

Product

Resources

About