Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

Hasada, Keiko; Yoshida, Toshihide; Yamazaki, Takeshi; Sugimoto, Naotoshi; Nishimura, Takuya; Nagatsu, Akito; Mizukami, Hajime

doi:10.1007/s11418-010-0393-x

Cited by 29 publications

(18 citation statements)

References 12 publications

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“…18 The LOQ values typically vary from 3 to 20 ng/mL in LC-MS methods. 20,21,26 However, the levels of LOD and LOQ values found in this study are comparable with other published applications of NMR in the field of pharmaceutical research, 27,28 including those using a higher magnetic field. 29,30 Although LOD and LOQ values may be lower using other methods (i.e., the HPLC method), the focus of this study is to quantify the amount of TNF release in biological fluid simulant rather than determine its aqueous solubility limit.…”

Section: Lod and Loqsupporting

confidence: 88%

Direct and Real-Time Quantification of Tenofovir Release from pH-Sensitive Microparticles into Simulated Biological Fluids Using 1H Nuclear Magnetic Resonance

Zhang

Oyler

et al. 2014

Journal of Pharmaceutical Sciences

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In vitro drug release evaluation is a very important step toward the quality control of nano- or micro-particular drug delivery systems. However, most quantitative techniques such as HPLC requires a dialysis membrane to separate the released free drug from these delivery systems, thus are not capable of direct detection and real-time quantification of the drug release. This study describes for the first time, a rapid, specific, and direct method for the real-time quantification of in vitro tenofovir (TNF) release from pH-sensitive microparticles using a Varian 400 MHZ 1H nuclear magnetic resonance (1H-NMR) spectrometer. Various analytical performance parameters such as linearity, precision, accuracy, limit of quantification (LOQ), limit of detection (LOD), and robustness were validated according to International Conference on Harmonization (ICH) guidelines. The in vitro release of TNF from microparticles in both simulated vaginal fluid (VFS) and the mixture (VSFS) of VFS and simulated semen fluid (SFS) was monitored and quantified in real-time using 1H-NMR. The capability of real-time quantification of in vitro drug release from microparticles not only provides a more accurate prediction of its biological behavior in vivo, but is also independent of potential interference from the dialysis membrane.

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Section: Lod and Loqsupporting

confidence: 88%

Direct and Real-Time Quantification of Tenofovir Release from pH-Sensitive Microparticles into Simulated Biological Fluids Using 1H Nuclear Magnetic Resonance

Zhang

Oyler

et al. 2014

Journal of Pharmaceutical Sciences

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“…The ethyl acetate fraction was concentrated under reduced pressure to yield each aglycone. The purity of each aglycone thus obtained was estimated by quantitative 1 H NMR analysis (24). Geniposide, genipin, and loganin were obtained from Wako Pure Chemicals (Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

Iridoid-specific Glucosyltransferase from Gardenia jasminoides

Nagatoshi

Terasaka

Nagatsu

et al. 2011

Journal of Biological Chemistry

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Iridoids are one of the most widely distributed secondary metabolites in higher plants. They are pharmacologically active principles in various medicinal plants and key intermediates in the biosynthesis of monoterpenoid indole alkaloids as well as quinoline alkaloids. Although most iridoids are present as 1-Oglucosides, the glucosylation step in the biosynthetic pathway has remained obscure. We isolated a cDNA coding for UDPglucose:iridoid glucosyltransferase (UGT85A24) from Gardenia jasminoides. UGT85A24 preferentially glucosylated the 1-O-hydroxyl group of 7-deoxyloganetin and genipin but exhibited only weak activity toward loganetin and no activity toward 7-deoxyloganetic acid. This suggests that, in the biosynthetic pathway of geniposide, a major iridoid compound in G. jasminoides, glucosylation occurs after methylation of 7-deoxyloganetic acid. UGT85A24 showed negligible activity toward any acceptor substrates other than iridoid aglycones. Thus, UGT85A24 has a remarkable specificity for iridoid aglycones. The mRNA level of UGT85A24 overlaps with the marked increase in genipin glucosylation activity in the methyl jasmonate-treated cell cultures of G. jasminoides and is related to iridoid accumulation in G. jasminoides fruits.

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“…In a previous paper, we reported the quantitative determination of the components of crude drugs by proton-specific quantitative NMR ( 1 HqNMR) [12][13][14][15][16]. The 1 H-qNMR method has various advantages over other quantitative methods, including HPLC.…”

Section: Introductionmentioning

confidence: 99%

Application of a quantitative 1H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan

et al. 2016

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Quantitative (1)H-NMR ((1)H-qNMR) was applied to the determination of paeonol concentration in Moutan cortex, Hachimijiogan, and Keishibukuryogan. Paeonol is a major component of Moutan cortex, and its purity was calculated from the ratio of the intensity of the paeonol H-3' signal at δ 6.41 ppm in methanol-d 4 or 6.40 ppm in methanol-d 4 + TFA-d to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material grade. As a result, the paeonol content in two lots of Moutan cortex as determined by (1)H-qNMR was found to be 1.59 % and 1.62 %, respectively, while the paeonol content in Hachimijiogan and Keishibukuryogan was 0.15 % and 0.22 %, respectively. The present study demonstrated that the (1)H-NMR method is useful for the quantitative analysis of crude drugs and Kampo formulas.

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Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

Cited by 29 publications

References 12 publications

Direct and Real-Time Quantification of Tenofovir Release from pH-Sensitive Microparticles into Simulated Biological Fluids Using 1H Nuclear Magnetic Resonance

Direct and Real-Time Quantification of Tenofovir Release from pH-Sensitive Microparticles into Simulated Biological Fluids Using 1H Nuclear Magnetic Resonance

Iridoid-specific Glucosyltransferase from Gardenia jasminoides

Application of a quantitative 1H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan

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