DNA barcode of matK combined with ITS effectively distinguishes the medicinal plant Stephania brachyandra Diels collected in Laocai, Vietnam

Abstract

In Vietnam, some species in the genus Stephania are being overexploited and recorded in the Red Data Book of Vietnam. In this article, we present the identification results of Stephania spp. collected in Lào Cai province using morphological characteristics and the DNA barcode method to contribute to the conservation and exploitation of genetic resources and pharmaceuticals of this species in Vietnam. The analysis of the data shows that all morphology characteristics and comparative anatomy of the petioles, stems, and leaves are typical of Stephania brachyandra Diels species. The matK gene and ITS region isolated from Stephania_ Laocai have base lengths of 879 bp and 423 bp, respectively. The (Bbasic Llocal Aalignment Ssearch Ttool) analysis of the matK gene and ITS region obtained in this study has the highest similarity, 99.37% and 98.97%, respectively, to S. brachyandra species. The matK sequences were highly conserved and had low variable sites for 747 nucleotides (84.98%) and 132 nucleotides (15.29%), respectively, whereas the short ITS region was less conserved and had variable sites for 78 (18.44%) and 345 (81.56%), respectively. The results of the molecular phylogenetic analysis of the matK gene by the maximum likelihood method for the Stephania_Laocai sample showed that the matK sequence is suggested for better phylogenetic resolution than the ITS region and the combination of the matK gene and ITS region can be used to identify S. brachyandra species. Based on the combination of the characteristics of morphology and nucleotide sequences of the matK gene and ITS region, Stephania spp. collected in Lào Cai province of Vietnam were determined as S. brachyandra Diels.

Evaluation of plastid and nuclear DNA markers in barcoding of Aloe saudiarabica, KSA

“…And through the different databases that were used, the sequences of these markers showed that the plant under study is of the genus Aloe. In many other recent studies, in which these markers were also used, and through which the ability of these markers to distinguish between plant species appeared with high efficiency (22,23). The results of the exploration through the matK genetic marker and using different primers showed that the identification was successful, as the genus Aloe, and especially the A. vera species, was the closest, perhaps, to the availability of the total identification of the genome of this plant.…”

ĐẶC ĐIỂM CỦA GENE rrn16S, trnK-UUU VÀ SỰ PHÁT SINH LOÀI Adinandra megaphylla Hu THU TẠI TỈNH LÀO CAI, VIỆT NAM

“…Vũ Thị Như Trang và cộng sự (2019) đã sử dụng mã vạch gene matK để nhận diện mẫu Thổ nhân sâm (Talinum paniculatum) thu tại một số địa phương phía bắc Việt Nam, đồng thời cũng xác định mối quan hệ di truyền giữa các loài Thổ nhân sâm nghiên cứu [9]. Năm 2021, Pham và cộng sự đã sử dụng mã vạch gene matK kết hợp với vùng ITS giúp phân biệt và xác định mối quan hệ di truyền cây thuốc Stephania brachyandra diels được thu thập tại tỉnh Lào Cai [10]. Huỳnh Thị Thu Huệ và cộng sự (2021) đã đánh giá khả năng phân loại của hai chỉ thị gene rbcL và trnL với một số mẫu Bách bộ (Stemonaceae) thu tại phía Bắc Việt Nam và chứng minh trình tự nucleotide kết hợp hai vùng chỉ thị mã vạch trên có thể là ứng viên mã vạch DNA cho định danh loài S. tuberose Lour [11].…”