DNA Barcoding Application for Seafood Label Traceability of Various Commercial Surimi-Based Products

Abdullah, Asadatun; Sativa, Hana Aulia; Nurhayati, ‪Tati; Nurilmala, Mala

doi:10.17844/jphpi.v22i3.28950

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…DNA isolation has an important role in the success of extraction of purification and quantity of DNA (Abdullah et al, 2019). The size of base pairs (bp) was the normal size of sea fish sequences, where the length of the base size in fish ranges from 200-1500 bp (Liu et al, 1999;Ali et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding and morphometric of Rastrelliger spp. in North Maluku Sea, Indonesia

Akbar¹,

Wibowo²,

Irfan³

et al. 2022

Aceh J. Anim. Sci.

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High exploration activity is feared to have an impact to mackerel populations. A sustainable management approach should be taken to provide information about the status of mackerel populations. Study of mackerel population status can be carried out through genetic information. The DNA of the fish samples were collected at traditional fish markets (Morotai, Bacan and Ternate). Laboratory works such as extraction, amplification, electrophoresis and DNA sequencing were analysis at the Indonesian Biodiversity Laboratory (Bionesia). The molecular characteristics of Rastrelliger kanagurta were 374 base pairs (bp). The composition of nucleotides showed the similarity of frequencies between species. Phylogenetic relationship of R. kanagurta in North Maluku Sea suggested that there was any differentiation. The genetic diversity of R. kanagurta was high with a total number of haplotypes and diverse nucleotide diversity. The minimum spawning networks (MSN) found 5 haplotype networks from a total of 12 samples. Morphological measurements of standard length, head height, body width, pectoral fin length and tail were found to have variable values. The length of the weight of the fish is obtained of b = 3, indicating that the growth pattern was isometric or weight gain was equivalent to the growth of the fish length.Keywords: Biodiversity, conservation, ecology, morphology, species

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Section: Discussionmentioning

confidence: 99%

DNA barcoding and morphometric of Rastrelliger spp. in North Maluku Sea, Indonesia

Akbar¹,

Wibowo²,

Irfan³

et al. 2022

Aceh J. Anim. Sci.

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show abstract

“…Mitochondrial DNA (mtDNA) is often used in DNA barcoding of many organisms, including fish (Abdullah et al 2019;Nurilmala et al 2020) because of the relatively small genome size. Also, mtDNA undergoes faster evolution making it more effective for the identification of adjacent interspecies (Amaral et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers

Nurilmala¹,

Ajitama²,

Jacoeb³

et al. 2020

Biodiversitas

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Abstract. Nurilmala M, Atjitama Y, Jacob AM, Indarwati AR, Nugraha R. 2020. Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers. Biodiversitas 21: 5650-5656. Shrimp crackers are snacks made from starch with the addition of shrimp and other ingredients. Authentication of shrimp crackers becomes a quality control method in line with its traceability since the morphological characters are not recognized in those products. The failure of the authentication will lead to adulteration of shrimp products. The study aimed to design shrimp-specific polymerase chain reaction (PCR) primers by and used them to authenticate shrimp cracker products to assure their traceability. The primers were designed from cytochrome oxidase subunit I (COI) gene with a GC content of 50% and a melting temperature of 57 °C. The amplicon had a length of 613 bp. Fourteen shrimp crackers having DNA concentrations 0.3 to 8.1 ng/µL were evaluated. Eight out of fourteen DNA samples were successfully amplified using the specific primer and could be visualized on the agarose gel at 500-750 bp. Those DNA samples were identified belonging to shrimp species, namely Litopenaeus vannamei, Metapenaeus ensis, and Fenneropenaeus merguiensis with 95% to 100% identity. Meanwhile, the other six DNA samples underwent PCR using universal primers. One sample was amplified and identified as fish (Sphyraena flavicauda). These results indicate illegal substitution of shrimp using unidentified species occurred in shrimp crackers.

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Application of mitochondrial gene markers and real-time PCR for the identification of Isurus spp. and Lamna nasus species

Abdullah,

Yatma,

Nurjanah

2024

BIO Web Conf.

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Sharks are predator species that play an important role in marine ecosystems and have high economic value. Exploiting shark parts, including fins, scales, teeth, cartilage, skin, liver, meat, and tails, is difficult to identify visually, necessitating molecular identification to determine traded shark products. This study aimed to design species-specific primers based on mitochondrial gene markers for shark species Isurus spp. and Lamna nasus, and their application in real-time PCR-based fisheries product authentication. The research was conducted in silico for specific primer design and in vitro for DNA sample isolation, DNA quantity and quality testing, DNA amplification, and real-time PCR analysis. The primer pairs of species included in the CITES II list with different genetic markers have been successfully created. The primers used were able to amplify DNA and showed a Ct difference between the target and non-target DNA, proving their ability to identify the target shark species.

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DNA Barcoding Application for Seafood Label Traceability of Various Commercial Surimi-Based Products

Cited by 7 publications

References 20 publications

DNA barcoding and morphometric of Rastrelliger spp. in North Maluku Sea, Indonesia

DNA barcoding and morphometric of Rastrelliger spp. in North Maluku Sea, Indonesia

Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers

Application of mitochondrial gene markers and real-time PCR for the identification of Isurus spp. and Lamna nasus species

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