DNA barcoding of the funoran-producing red algal genus Gloiopeltis (Gigartinales) and description of a new species, Gloiopeltis frutex sp. nov.

Identification of true Gloiopeltis furcata (Gigartinales, Rhodophyta) and preliminary analysis of its biogeography

“…, Kraft & Saunders , Saunders , Yang et al . , Yang & Kim ). Endocladia muricata (Endlicher) J. Agardh and Gloiosiphonia sp.…”

“…After PEG purification (Lis 1980), PCR products were Sanger sequenced using a CE DTCS Quick Start Kit (Beckman Coulter, Fullerton, CA, USA) and a CEQ8000 DNA analysis system (Beckman Coulter) according to the manufacturer's instructions, or sequenced by a DNA sequencing service (FASMAC, Atsugi, Japan). For the molecular phylogenetic analyses, published and newly determined sequence data were used (Table S1 in the Supporting Information; Freshwater et al 1994, Krayesky et al 2009, Kraft & Saunders 2014, Saunders 2014, Yang et al 2016, Yang & Kim 2018. Endocladia muricata (Endlicher) J. Agardh and Gloiosiphonia sp.…”

“…Endocladia muricata (Endlicher) J. Agardh and Gloiosiphonia sp. were chosen as outgroups, because these two genera are closely related to the genus Gloiopeltis (Yang & Kim 2018). Alignments were prepared using the program MAFFT v.6 (Katoh & Toh 2008) and then manually adjusted prior to phylogenetic analyses.…”

“…Preliminary genetic analyses using mitochondrial cox1 and plastid rbcL gene sequences from Japanese specimens have suggested greater species diversity in the genus than currently recognized, especially in those specimens morphologically referable to G. furcata. Yang and Kim (2018) showed that the specimens from Korea and Japan identified as G. furcata by morphology were split into two clades. Our own preliminary analyses revealed six clades supported by high statistical values (see below and Fig.…”

Incorporation of yellow onion peel extract into the funoran‐based biodegradable films as an antioxidant packaging material

“…Additionally, GF extract is reported to exhibit functional properties, such as anti-inflammatory and anti-tumour activities (Hu et al, 2012). Previously, the polysaccharide isolated from GF was reported to be a galactan mixture, which is called funoran (Yu et al, 2010;Yang & Kim, 2018). Funoran is a heterogeneous sulphated galacto polysaccharide with 3,6-anhydro-a-L-galactose and b-D-galactose-6-sulphate repeating units (Tuvikene et al, 2015).…”