DNA–Carbon Nanotube Conjugates Prepared by a Versatile Method Using Streptavidin–Biotin Recognition

Lyonnais, Sébastien; Goux-Capes, Laurence; Escudé, Christophe; Côte, Denis; Filoramo, Arianna; Bourgoin, Jean‐Philippe

doi:10.1002/smll.200700586

Cited by 22 publications

(23 citation statements)

References 35 publications

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“…31 This adsorption of streptavidin on the surface of the SWNTs can be used to link other molecules to the nanotubes, using the high specific bonding between biotin and streptavidin. Lyionnais et al 51 have used this concept to prepare DNA-Carbon nanotubes conjugates. Bottini et al 52 have used streptavidin as an interlinker between SWNTs and quantum dots (QD).…”

Section: Colorimetric Test Resultsmentioning

confidence: 98%

Non-Specific Adsorption of Streptavidin on Single Walled Carbon Nanotubes

González¹,

Tort²,

Benito³

et al. 2009

J. Nanosci. Nanotech.

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We report the semi quantitative determination of the non specific adsorption of streptavidin onto raw and functionalized single walled nanotubes (SWNTs) using different approaches. SWNTs were covalently functionalized with carboxylated groups, amine groups and polyethylene glycol (PEG), and non-covalently functionalized with polyethylene glycol, Pluronic F-127 and Tween20. SWNTs covalent functionalization has been carried out through oxidation and acyl chloride formation. The non covalent functionalization was carried out by SWNTs dispersion in aqueous solution of the corresponding surfactant or polymer. A colorimetric test based on the horseradish peroxidase-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) was used to determine the presence of streptavidin onto the SWNTs. Streptavidin adsorption was also carried out by covalent bonding of biotin to SWNTs, to compare the specific and non-specific adsorption. Non specific streptavidin adsorption has been observed on pristine and all types of functionalized SWNTs tested. This adsorption of streptavidin on the surface of the SWNTs could be used to link other biomolecules to the nanotubes, using the high specific bonding between biotin and streptavidin.

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Section: Colorimetric Test Resultsmentioning

confidence: 98%

Non-Specific Adsorption of Streptavidin on Single Walled Carbon Nanotubes

González¹,

Tort²,

Benito³

et al. 2009

J. Nanosci. Nanotech.

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“…Moreover, in DNA-directed nanoelectronics, DNA can be envisioned not only as a positioning scaffold, but also as a support for the conducting elements. [32,33] With the aim to fabricate carbon nanotube-based devices by combining DNA with SWNTs, we developed a non-covalent approach based on biotin-streptavidin molecular recognition properties [9][10][11] that preserves the structural quality of the CNTs (Fig. 4).…”

Section: Selective Placement Of Nanotubesmentioning

confidence: 99%

“…biotin at tagged locations along DNA molecules [10] or close to the intersection site of T-shape DNA structures. [11] Such synthesis of three branch DNA scaffolds and the insertion of CNTs at their centre are the first steps toward the bio-directed selfassembly of nanotube transistors with individual gates.…”

Section: Selective Placement Of Nanotubesmentioning

confidence: 99%

“…In the past, a strategy was developed at the Laboratory of Molecular Electronics of the CEA Saclay to build and study electronic devices, starting from commercial nanotubes and going through different steps of chemical purification, [2,3] solubilization, functionalization, [3,4] and assembly. [5][6][7][8][9][10][11] We considered these steps as the key to the development of innovative electronic functions. In particular, we studied carbon nanotube field effect transistors and their chemical optimization, [12,13] high frequency nanotube transistors, [14][15][16] nanotubebased nano-electromechanical systems (NEMS), [17,18] and nanotube-based optoelectronic devices with memory capabilities and related computing architectures.…”

Section: Introductionmentioning

confidence: 99%

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Recent Advances in Molecular Electronics Based on Carbon Nanotubes

Bourgoin

Campidelli²,

Chenevier³

et al. 2010

Chimia

Self Cite

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Carbon nanotubes (CNTs) have exceptional physical properties that make them one of the most promising building blocks for future nanotechnologies. They may in particular play an important role in the development of innovative electronic devices in the fields of flexible electronics, ultra-high sensitivity sensors, high frequency electronics, opto-electronics, energy sources and nano-electromechanical systems (NEMS). Proofs of concept of several high performance devices already exist, usually at the single device level, but there remain many serious scientific issues to be solved before the viability of such routes can be evaluated. In particular, the main concern regards the controlled synthesis and positioning of nanotubes. In our opinion, truly innovative use of these nano-objects will come from: (i) the combination of some of their complementary physical properties, such as combining their electrical and mechanical properties, (ii) the combination of their properties with additional benefits coming from other molecules grafted on the nanotubes, and (iii) the use of chemically- or bio-directed self-assembly processes to allow the efficient combination of several devices into functional arrays or circuits. In this article, we outline the main issues concerning the development of carbon nanotubes based electronics applications and review our recent results in the field.

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“…The challenge is to find an efficient way to integrate dense bundles of oriented CNTs at wafer scale. Several techniques are currently investigated [3][4][5][6][7]. One of the problems to obtain devices with controlled behaviours is the large dispersion of conduction properties observed in CNT samples.…”

Section: Introductionmentioning

confidence: 99%

A simple and versatile method for statistical analysis of the electrical properties of individual double walled carbon nanotubes

Seichepine

Flahaut

Vieu

2011

Microelectronic Engineering

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International audienceDouble-walled carbon nanotubes (DWNTs) are potential candidates for new generation of on chip interconnections due to their nearly metallic behaviour. For such large scale integration purpose it is mandatory to characterize their electrical properties in a statistical way. We thus propose a new methodology for characterizing in one step, the electrical properties of a large population of nanotubes. The method enables to obtain histograms of the conductance and maximum current density of individual nanoobjects

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DNA–Carbon Nanotube Conjugates Prepared by a Versatile Method Using Streptavidin–Biotin Recognition

Cited by 22 publications

References 35 publications

Non-Specific Adsorption of Streptavidin on Single Walled Carbon Nanotubes

Non-Specific Adsorption of Streptavidin on Single Walled Carbon Nanotubes

Recent Advances in Molecular Electronics Based on Carbon Nanotubes

A simple and versatile method for statistical analysis of the electrical properties of individual double walled carbon nanotubes

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