DNA damage, acetylcholinesterase activity, and hematological responses in rainbow trout exposed to the organophosphate malathion

Alavinia, Seyed Jalil; Mirvaghefi, Alireza; Farahmand, Hamid; Rafiee, Gholamreza; Alavinia, Seyed Jamal; Shiry, Nima; Moodi, Saeed

doi:10.1016/j.ecoenv.2019.05.081

Cited by 28 publications

(5 citation statements)

References 33 publications

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“…Pesticides exposed to fish at lethal concentrations of EMB expressed abnormal behaviour like lethargy, rapid opercular movement, loss of balance, excess mucus secretion, and darkening on the body surface. However, no visible symptom was observed in the fish exposed to EMB during the sub-lethal toxicity test for a period of 72 h. In an earlier study, erratic and irregular swimming movements, loss of balance, sudden overexcitability were observed at 0.093 mg L −1 of malathion in rainbow trout [ 46 ]; rapid swimming, higher opercular movements were noticed in cypermethrin treated L. Rohita [ 47 ]; erratic behaviour with sluggish swimming patterns, loss of balance and elevation of opercular respiration in C. carpio exposed to glyphosate [ 48 ]. EMB and abamectin induced hypoactivity in Danio rerio , Zebrafish [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of the effect of sub-lethal acute toxicity of Emamectin benzoate in Labeo rohita using multiple biomarker approach

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Assessment of the effect of sub-lethal acute toxicity of Emamectin benzoate in Labeo rohita using multiple biomarker approach

et al. 2022

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“…The activity of AChE may be altered due to exposing of different pollutants. AChE inhibition or degradation of acetylcholine increases the excitation of cholinergic nerves (Alavinia et al, 2019; Mohamed et al, 2020). In the current research, the alteration of AChE activity and BDNF levels of O. mykiss exposure to AFC may be interpreted as neurological dysfunction and oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Ulexite modulates the neurotoxicological outcomes of acetylferrocene‐exposed rainbow trout

Uçar

Özgeriş

Parlak

et al. 2022

Environ and Mol Mutagen

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In this study, the neuroprotective action potential by ulexite (UX) (18.75 mg/L) against acetylferrocene (AFC) (3.82 mg/L) induced neurotoxicity was aimed to investigate in brain tissues of Oncorhynchus mykiss. For this purpose, the effects on neurotoxicity markers, proinflammatory cytokines, antioxidant immune system, DNA, and apoptosis mechanisms were assessed on brain tissues in the 48-96 h of the 96-trial period. In this research, it was determined that brain-derived nerve cell growth factor (BDNF) level and acetylcholinesterase (AChE) activity were inhibited in the brain tissue compared to the control group by AFC. In addition, inhibition in glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) values (which are antioxidant system biomarkers), and inductions in malondialdehyde (MDA) and myeloperoxidase (MPO) amounts (which are indicators of lipid peroxidation) were determined (p < 0.05) after exposure to AFC. And, while tumor necrosis factor-α (TNF-α) and IL-6 levels were increased in the AFC-exposed group, Nrf-2 levels were found to be remarkably decreased. Upregulation was also detected in 8-hydroxydeoxyguanosine (8-OHdG) and caspase-3 levels, which are related to DNA damage and apoptosis mechanism. On the contrary, UX (single/with AFC) suppressed the AChE and BDNF inhibition by AFC. Moreover, UX mitigated AFC-induced oxidative, inflammatory, and DNA damage and attenuated AFC-mediated neurotoxicity via activating Nrf2 signaling in fish. Collectively, our findings revealed that UX supplementation might exert beneficial effects and may be considered as a natural and promising neuroprotective agent against AFC-induced toxicity.

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“…Dimethoate-induced oxidative stress, and DNA damage has been seen in rat's brain and liver (Yahia & Ali, 2018). Effects of malathion, an OP on rainbow trout, at sub-lethal concentrations caused DNA damage (Alavinia et al, 2019). Another OP insecticide, chlorpyrifos, causes DNA damage in HeLa and HEK293 cells in vitro.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress, DNA damage, and histological alterations in Bombyx mori exposed orally to pesticide dimethoate

Qadri

Qamar

Maheshwari

2022

Physiological Entomology

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Pesticides are an essential part of agricultural practices that ward off pathogens and diseases from the agricultural crop. However, apart from target organisms, these chemicals also have adverse effects on non‐target organisms. Dimethoate is an insecticide used extensively in agriculture and horticulture practices worldwide. We used the silkworm Bombyx mori as a model organism to study the effect of commercial formulation of dimethoate (Dimethoate‐30% EC) on the gut, silk gland, and fat body tissues. LD50 of dimethoate‐30% EC on silkworm (B. mori) was 997 ppm, as reported in a previous study. We used concentrations of 25, 50, and 100 ppm in our experiments. Our results showed that sub‐lethal doses of dimethoate caused weight loss and induced damage at the histological level to the mid‐gut, silk gland, and fat body of B. mori. It also caused a decrease in the level of antioxidants like CAT, SOD, GPx, GSH, and GST, indicating that dimethoate has produced a shift of ROS balance towards free radical generation and therefore resulted in overall damage to this organism. Sub‐lethal doses of this pesticide also caused lipid peroxidation in the silk gland, gut, and fat body of B. mori, damaging these tissues. The disruption was also seen in the mid‐gut and middle silk gland at the DNA level, where it caused single‐strand breaks, as was revealed by single cell gel electrophoresis studies. Damage at histological, biochemical, and molecular levels was most extreme at a concentration of 100 ppm, the highest sub‐lethal concentration given to B. mori.

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DNA damage, acetylcholinesterase activity, and hematological responses in rainbow trout exposed to the organophosphate malathion

Cited by 28 publications

References 33 publications

Assessment of the effect of sub-lethal acute toxicity of Emamectin benzoate in Labeo rohita using multiple biomarker approach

Assessment of the effect of sub-lethal acute toxicity of Emamectin benzoate in Labeo rohita using multiple biomarker approach

Ulexite modulates the neurotoxicological outcomes of acetylferrocene‐exposed rainbow trout

Oxidative stress, DNA damage, and histological alterations in Bombyx mori exposed orally to pesticide dimethoate

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