DNA extraction from dry wood of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling and timber tracking

Tnah, Lee Hong; Lee, Soon Leong; Ng, Kevin Kit Siong; Bhassu, Subha; Othman, Rofina Yasmin

doi:10.1007/s00226-011-0447-6

Cited by 26 publications

(26 citation statements)

References 27 publications

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“…DNA extraction from fresh and dried leaves is a common practice in plant genetic studies. Nevertheless, few studies have reported on DNA extraction from dry wood (Tnah et al 2012) as it is still the major challenge. Once this obstacle is overcome, DNA fingerprinting technology is potentially an efficient tool to identify the origins of timber and track logs along supply chains.…”

Section: Introductionmentioning

confidence: 99%

“…(DumolinLapègue et al 1999;Deguilloux et al 2002), Gonystylus bancanus (Miq.) Kurz (Asif and Cannon 2005), and various dipterocarps (Tnah et al 2012;Rachmayanti et al 2006Rachmayanti et al , 2009Yoshida et al 2007). Liepelt et al (2006) suggested that DNA extracted from ancient Abies Mill., Pinus L., Fagus L., and Quercus wood samples could also be used in genetic studies.…”

Section: Introductionmentioning

confidence: 99%

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Tracking the geographical origin of timber by DNA fingerprinting: a study of the endangered species Cinnamomum kanehirae in Taiwan

Hung

Lin

2017

Holzforschung

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Cinnamomum kanehirae Hay. is endemic in Taiwan and is severely threatened due to intensive utilization and illegal logging. To combat illegal logging, suitable identification markers are needed, which are usable in a court of law, such as microsatellite marker for genotyping. In the present paper, a genetic fingerprinting database was generated based on 15 microsatellites, which are suitable to assess the timber's origin and its population genetic structure. The quality of DNA extractions from C. kanehirae timbers was assessed by comparing cpDNA trnL-trnF sequence lengths. The cumulative probability of identifying unrelated individuals in these microsatellites was 5.151 × 10 −17. The results indicate that the low genetic diversity is a consequence of illegal logging and that there is a significant genetic differentiation among C. kanehirae populations. It was possible to trace back the geographical origin of unknown C. kanehirae timbers based on a genetic reference database, i.e. all blind wood samples were assigned to their true geographical origins. Accordingly, microsatellites are a useful tool to identify the population origins of timbers and can be considered as a tool for combating illegal logging of C. kanehirae.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tracking the geographical origin of timber by DNA fingerprinting: a study of the endangered species Cinnamomum kanehirae in Taiwan

Hung

Lin

2017

Holzforschung

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“…This might be because cp DNA was a high copy number in the cells based on sapwood. Many studies reported that the less potential inhibitors of DNA isolation and PCR amplification are present in the sapwood (Rachmayanti et al 2006;Tnah et al 2012;Yu et al 2016). There are many more living parenchyma cells in the sapwood than in the heartwood (Jiao et al 2014;Yu et al 2016).…”

Section: Indel Predictions and Identification Of C Micranthum And Cmentioning

confidence: 99%

Comparative analysis of the complete chloroplast genomic sequence and chemical components of Cinnamomum micranthum and Cinnamomum kanehirae

Chu

et al. 2017

Holzforschung

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Cinnamomum micranthum (Hayata) Hayata is a tree species that is often confused with Cinnamomum kanehirae Hayata, which is an endemic species in Taiwan and the sole natural host of the valuable medicinal fungus Antrodia cinnamomea. However, the two species are highly similar in morphology and difficult to distinguish based on traditional vegetative structures or wood anatomical methods. The aim of the present study was to identify of these two species by DNA and chemical analysis. The complete chloroplast genome of C. micranthum has been determined and compared with that of C. kanehirae. The leaf methanol extracts of these species were also analyzed by ultra-performance liquid chromatography (UPLC). The length of the C. micranthum chloroplast genome was 152 675 bp, i.e. 25 bp shorter than that of C. kanehirae. In phylogenetic analysis, C. micranthum was more closely related to C. kanehirae than other six Lauracea species. Six validated insertion/deletions of bases in the DNA (InDels) are suitable for differentiation of the two species. Moreover, high amounts of linalool and sesamin were present in the leaves of C. kanehirae, but not in C. micranthum. Overall, this study provided new insight for distinguishing the two species on the basis of the chemical composition of the leaves and the InDel markers.

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“…Expert opinion based on genetic fingerprinting methods now suggests that these techniques provide a level of basic proof acceptable in criminal cases in line with the Penal Code; in forestry, this evidence enables those people who take felled trees away from a forest for the purpose of appropriation to be subject to the same liability as if they had engaged in stealing. Courts now accept that genetic profiles based on nuclear or plastid markers provide rapid and robust tools for comparison between evidence (foliage, wood) and material of reference (tree stumps) in the forest (Tnah et al 2012;Nowakowska and Pasternak 2014). It should be emphasized, however, that a minimum of 4-6 highly polymorphic nuclear markers, that possess between five and 15 alleles each, are required to provide suitable discriminatory power in forensics (Butler 2005;Goodwin et al 2011).…”

Section: Power Of Discrimination Of the Markersmentioning

confidence: 99%

“…To prevent illegally harvested timber and products derived from such timber appearing on the market, genetic tools based on DNA markers have been developed (Asif and Cannon 2005;Deguilloux et al 2002Deguilloux et al , 2003Tnah et al 2012). These DNA markers are additional tools to be used alongside classical methods such as mechanoscopy and dendrochronology, which are often applied to investigate different forms of crime in forests (Yaman and Akkemik 2009); especially, when mechanoscopy or dendrochronology fails to provide sufficient evidence in investigations, i.e.…”

Section: Introductionmentioning

confidence: 99%

Analysis of DNA profiles of ash (Fraxinus excelsior L.) to provide evidence of illegal logging

Tereba¹,

Woodward

Konecka³

et al. 2017

Wood Sci Technol

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Illegal logging is a major problem in many European countries. Recent progress in molecular biology, however, has significantly improved the ability to accurately identify wood material. In this paper, the first application of microsatellite DNA markers is described in a case of illegal logging of European ash wood in Polish State Forests. The genetic fingerprints of seized ash wood samples were determined using six nuclear and four chloroplast microsatellite loci, characterized by sufficient stringency in forensic analyses. By comparing the DNA profiles obtained, the origin of one sample of ash wood used as evidence material was confirmed, from among three samples serving as reference material with 99.99999% of probability. This work demonstrates how DNA authenticity testing can serve as an important technical tool in monitoring the legality of the suspected ash timber and confirms the utility of these techniques in detecting illegally logged timber in general.

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DNA extraction from dry wood of Neobalanocarpus heimii (Dipterocarpaceae) for forensic DNA profiling and timber tracking

Cited by 26 publications

References 27 publications

Tracking the geographical origin of timber by DNA fingerprinting: a study of the endangered species Cinnamomum kanehirae in Taiwan

Tracking the geographical origin of timber by DNA fingerprinting: a study of the endangered species Cinnamomum kanehirae in Taiwan

Comparative analysis of the complete chloroplast genomic sequence and chemical components of Cinnamomum micranthum and Cinnamomum kanehirae

Analysis of DNA profiles of ash (Fraxinus excelsior L.) to provide evidence of illegal logging

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