DNA hybridization using surface plasmon-coupled emission

Malicka, Joanna; Gryczyński, Ignacy; Gryczyński, Zygmunt; Lakowicz, Joseph R.

doi:10.1117/12.530001

Cited by 18 publications

(25 citation statements)

References 19 publications

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“…The enhanced excitation field is confined to the evanescent layer, effectively reducing the background from the sample volume matrix. The exceptional sensitivity of SPCE-based assays has been observed in our and other laboratories (42)(43)(44)(45)(46)(47)(48).…”

supporting

confidence: 54%

Plasmonic Technology: Novel Approach to Ultrasensitive Immunoassays

Lakowicz¹,

Malicka²,

Matveeva³

et al. 2005

Clinical Chemistry

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, we have taken advantage of the favorable properties of surface plasmon-coupled emission (SPCE) to improve fluorescence-based immunoassays. SPCE occurs when excited fluorophores near conducting metallic structures efficiently couple to surface plasmons. These surface plasmons, appearing as free electron oscillations in the metallic layer, produce electromagnetic radiation that preserves the spectral properties of fluorophores but is highly polarized and directional. SPCE immunoassays provide several advantages over other fluorescencebased methods. This review explains new approaches to fluorescence immunoassays, including our own use of SPCE for simultaneous detection of more than one fluorescent marker and performance of immunoassays in the presence of an optically dense medium, such as whole blood. © 2005 American Association for Clinical ChemistryBiological markers in physiologic fluids are very useful tools in clinical diagnostics. Sensitive and reliable detection of specific biomarkers is crucial in disease identification, therapy, and patient screening. Reliable and quick detection of low concentrations of markers is particularly important in a disease such as acute myocardial infarction (AMI).1 Myoglobin (Myo), although not completely cardiac specific, is one of the earliest markers to increase after AMI (1-6 ) and has been recommended for use in combination with other markers, such as creatine kinase-MB, troponin I, and troponin T, as an early diagnostic indicator for AMI (1,(7)(8)(9)(10). The immunoassay technique is widely used in this procedure (11 ).Immunoassay based on fluorescence detection is one approach to high-sensitivity detection of biomarkers (12)(13)(14)(15)(16) . Different fluorescence detection approaches include polarization (17-21 ), resonance energy transfer (22-24 ), and time-resolved gated assays based on long-lived lanthanide emission (25-28 ). New approaches to fluorescence immunoassays are being developed, including multiphoton excitation (29 -31 ), with the emphasis on highthroughput immunoassays (32-35 ).The sensitivity of fluoroimmunoassays is typically limited by background fluorescence, which is present in most biological samples and in the optical elements of the instrumentation. In this report, we describe a new immunoassay format that provides increased sensitivity and background rejection by efficient light collection of emissions occurring near the bioaffinity surface. In this approach, a fluorescently labeled reporting molecule (antibody) is bound near a metallic surface, and the binding produces a highly directional and polarized emission. This effect is based on the resonant coupling of excited fluorophores with collective electron motions/oscillations at the interface between a thin metal film (typically silver or gold) and a dielectric bulk material. The so-called surface plasmons comprise an electromagnetic wave confined to the interface between the metal film and the transparent medium, and the electromagnetic wave of the surface plasmons is coupled to oscillatio...

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supporting

confidence: 54%

Plasmonic Technology: Novel Approach to Ultrasensitive Immunoassays

Lakowicz¹,

Malicka²,

Matveeva³

et al. 2005

Clinical Chemistry

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“…Both the SPR and SPCE angles are highly sensitive to the thickness of the dielectric layer (sample) above the metal. This fact has been used in surface plasmon resonance analysis (SPRA) for detection of bioaffinity reaction on surfaces (13)(14)(15)(16) and in SPCE for bioassay studies (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon–coupled Polarized Emission of N-Acetyl-l-Tryptophanamide¶

Gryczyński¹,

Malicka²,

Łukomska³

et al. 2004

Photochem Photobiol

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We report an observation of ultraviolet (UV) surface plasmon-coupled emission (SPCE) of N-acetyl-l-tryptophanamide (NATA). The sample was spin coated from poly(vinyl alcohol) (PVA) solution on 20 nm aluminum film deposited on a quartz substrate. The directional UV SPCE occurs within a well-defined narrow angle at 52 degrees from the normal to the coupling hemicylinder quartz prism. The NATA directional emission is highly p polarized as expected for surface plasmon-coupled radiation. The 10 nm protective SiO2 layer deposited on top of the aluminum film significantly neutralized the fluorophore quenching by the metal surface. SPCE of NATA demonstrates a remarkable intrinsic dispersive property-the maximum of the emission spectrum depends on the observation angle. The efficient spectral resolution of SPCE can be used in the construction of miniaturized spectrofluorometers. The observation of SPCE of tryptophan opens a new possibility for the study of many unlabeled proteins with the technique complementary to surface plasmon resonance analysis.

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“…The simulation results were comparable to the experimental SPCE results of Gryczynski, Lakowicz, and Malicka. 22,24,30,31 Qi and coworkers' simulations were based on Fresnel's equations and optical reciprocity theorem. 28,29 They simulated a plasmon waveguide structure in the Kretschmann configuration with a dipole emitter positioned at various locations within the waveguide dielectric layer, which was placed between a gold film and an air layer.…”

Section: Introductionmentioning

confidence: 99%

Experimental analysis of waveguide-coupled surface-plasmon-polariton cone properties

Nyamekye

Zhu

Mahmood

et al. 2019

Analytica Chimica Acta

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Experimental data for waveguide-coupled surface-plasmon-polariton (SPP) cones generated from dielectric waveguides is presented. The results demonstrate a simpler route to collect plasmon waveguide resonance (i.e., PWR) data. In the reverse-Kretschmann configuration (illumination from the sample side) and Kretschmann configuration (illumination from the prism side), all the waveguide modes are excited simultaneously with p-or s-polarized incident light, which permits rapid acquisition of PWR data without the need to scan the incident angle or wavelength, in the former configuration. The concentric SPP cone properties depend on the thickness and index of refraction of the waveguide. The angular intensity pattern of the cone is well-matched to simulation results in the reverse-Kretschmann configuration, and is found to be dependent on the polarization of the incident light and the polarization of the waveguide mode. In the Kretschmann geometry, all waveguide-coupled SPP cones are measured at incident angles that produce attenuated light reflectivity. In addition, the enhanced electric field produced under total internal reflection allows high signal-to-noise ratio multimodal spectroscopies (e.g., Raman scattering, luminescence) to measure the chemical content of the waveguide film, which traditionally is not measured with PWR. AbstractExperimental data for waveguide-coupled surface-plasmon-polariton (SPP) cones generated from dielectric waveguides is presented. The results demonstrate a simpler route to collect plasmon waveguide resonance (i.e., PWR) data. In the reverse-Kretschmann configuration (illumination from the sample side) and Kretschmann configuration (illumination from the prism side), all the waveguide modes are excited simultaneously with p-or s-polarized incident light, which permits rapid acquisition of PWR data without the need to scan the incident angle or wavelength. The concentric SPP cone properties depend on the thickness and index of refraction of the waveguide. The angular intensity pattern of the cone is well-matched to simulation results in the reverse-Kretschmann configuration, and is found to be dependent on the polarization of the incident light and the polarization of the waveguide mode. In the Kretschmann geometry, all waveguide-coupled SPP cones are measured at incident angles that produce attenuated light reflectivity. In addition, the enhanced electric field produced under total internal reflection allows high signal-to-noise ratio multimodal spectroscopies (e.g., Raman scattering, luminescence) to measure the chemical content of the waveguide film, which traditionally is not measured with PWR.

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DNA hybridization using surface plasmon-coupled emission

Cited by 18 publications

References 19 publications

Plasmonic Technology: Novel Approach to Ultrasensitive Immunoassays

Plasmonic Technology: Novel Approach to Ultrasensitive Immunoassays

Surface Plasmon–coupled Polarized Emission of N-Acetyl-l-Tryptophanamide¶

Experimental analysis of waveguide-coupled surface-plasmon-polariton cone properties

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