DNA polymorphism at locus-2 of growth hormone gene of Madura cattle

Purwoko, Agus; Sutarno, Sutarno; Etikawati, Nita

doi:10.13057/biodiv/d040102

Cited by 5 publications

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“…This was evident that the frequencies of allele and genotype of GH gene in the four exons of Pesisir cattle having value less than 0.99 except in exon 2 which was monomorphic. GH gene polymorphism was found in samples of Madura cattle was consistent with the results of research of Purwoko et al (2003), which stated that there were polymorphisms in the GH gene locus 2 which was positioned at 329 bp. About Katingan cattle, the results of the study found that there were variations in frequencies alleles namely polymorphic in exon 1, 2, 4 and 5, except exon 3 which was monomorphic.…”

Section: Frequencies Of Genotype and Allele Gh Genesupporting

confidence: 89%

Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method

Rahmatullah

2016

Biodiversitas

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The aim of this study was to identify the polymorphisms of the growth hormone gene (GH) of Indonesian local cattle as well as two exotic cattle, as the outside group, using polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) and using five primers DNA to identify polymorphism of GH gene. Twenty DNA samples of each Indonesian local cattle, consists of Bali, Pesisir, Madura and Katingan, and ten DNA samples of Simmental and Limousine cattle were used. The results showed that the polymorphism of the GH gene was found in three exons which are exon 1, 2 and 5 for Indonesian local cattle except for the Bali and Madura cattle that showed polymorphism was only at exon 2. Bali and Madura cattle also showed monomorphism in exon 3 and 4. On the other hand, the exotic breed showed the polymorphism in all exons, except for exon 2 in Simmental cattle which was found to be monomorphic.

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Section: Frequencies Of Genotype and Allele Gh Genesupporting

confidence: 89%

Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method

Rahmatullah

2016

Biodiversitas

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“…The study of GH gene MspI locus of Indonesian beef cattle have been reported in Ongole crossbred cattle (Sutarno et al, 2005;Sutarno, 2010), Madura cattle (Purwoko et al, 2003), Pesisir cattle (Jakaria et al, 2007), Bali cattle (Jakaria et al, 2009) and Grati dairy cows (Maylinda, 2011). Their studies indicated that polymorphism was found on Ongole crossbred cattle, Madura cattle, Pesisir cattle and Grati dairy cows.…”

Section: Introductionmentioning

confidence: 99%

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

Putra

Hartatik

Sumadi

2013

J. Indonesian Trop. Anim. Agric.

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ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi genotip gen growth hormone (GH) pada sapi Aceh yang diseleksi di Balai Pembibitan Ternak Unggul -Hijauan Pakan Ternak (BPTU-HPT) Sapi Aceh Indrapuri. Empat puluh satu ekor sapi Aceh terdiri dari 21 jantan dan 20 betina digunakan dalam penelitian ini. Ekstraksi DNA genom menggunakan darah sesuai metode Sambrook et al. (1989). Analisis Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) dan sekuensing digunakan untuk melihat situs restriksi MspI pada gen GH. Berdasarkan hasil sekuensing diketahui bahwa semua sampel sapi Aceh bersifat monomorfisme. Frekuensi genotip TT dan alel T masing-masing sebesar 1,00. Mutasi transisi C (cytosine) menjadi T (thymine) pada posisi 1548 bp menyebabkan situs restriksi MspI tidak ditemukan. Kata kunci: Sapi Aceh, gen GH, PCR-RFLP, sekuensing, enzim restriksi MspI ABSTRACTThe objective of this research was to identify growth hormone (GH) genes genotype in selected Aceh cattle at Indrapuri's Breeding and Forage Centre (IBFC) of Aceh Cattle. Fourty one cattle consisting of 21 male and 20 female cattle were used in this study. The genomic DNA was extracted from blood using Sambrook et al. (1989) methods. Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) and mehod of sequencing was used to detect MspI site on GH gene. Based on sequencing results, it can be concluded that all cattle were monomorphism. The frequency of TT genotype were 1.00 and same as T allele frequency. The transition of C (cytosine) into T (thymine) on 1548 bp caused the lost of restriction site.

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“…The relationship of genotype variations in growth hormone locus with a total growth of cattle is probably caused by differences of growth hormone circulation as a result of the growth hormone gene variation (Sutarno 2006). Study of gene polymorphisms of growth hormone has also been done on some Indonesian local cattle such as Pesisir cattle (Jakaria et al 2007), Bali cattle (Jakaria et al 2009;Jakaria and Noor 2011), Madura cattle (Purwoko et al 2003;Hartatik et al 2013), PO cattle (Sutarno et al 2005;Sutarno 2010;Paputungan et al 2013;Rahayu et al 2014), Sumba Ongole cattle (Anwar et al 2015), Aceh cattle (Putra et al 2013(Putra et al , 2014 and Grati dairy cattle (Maylinda 2011).…”

Section: Growth Hormone Genementioning

confidence: 99%

Review: Genetic diversity of local and exotic cattle and their crossbreeding impact on the quality of Indonesian cattle

Sutarno

Setyawan

2015

Biodiversitas

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Sutarno, Setyawan AD. 2015. Genetic diversity of local and exotic cattle and their crossbreeding impact on the quality of Indonesian cattle. Biodiversita16: 327-354.Several species of cattle had been domesticated around the world, but only two species werefarmed extensively, zebu cattle (Bos indicus) of the tropics and taurine cattle (Bos taurus) of the subtropical areas. Both of them hadhundreds variety of offspring in the worlds. The third species of cattle that most widely farmed was Bali cattle (Bos javanicus), anindigenous cattle from Indonesia that was domesticated from wild banteng (Bos javanicus javanicus). Besides Bali cattle, Indonesia hadalso some local cattle as direct descendants of or as Crossbreeds of those three cattle. These cattle had been adapted to climaticconditions, feeds and diseases in Indonesia. Local zebu cattle that relatively pure were Peranakan Ongole (PO) or Ongole breeds andSumba Ongole (SO). The main Crossbreed between zebu and Bali cattle was Madura cattle. The other well-known cattle of this wereAceh cattle, Pesisir cattle, Rancah cattle, Jabres cattle, Galekan cattle and Rambon cattle. Crossbreeds of taurine and zebu cattlegenerally produced calf that declining reproductive ability in generations. One fairly successful was Grati cattle or Holstein FreisianIndonesia (FHI) which was a crossbreed of Holstein Friesian and PO cattle. In recent decades, there were many crossbreed activitiesthrough artificial insemination between local cattle and taurine cattle to produce excellent beef cattle, mainly Simmental and Limousin.This activity was carried out widely and evenly distributed throughout Indonesia. It was conducted on all local cattle breeds and wasstrongly supported by local farmers. This crossbreeding activity was feared to change the genetic diversity of local Indonesia cattle,where the descendants could not adapt to the climatic conditions, feeds and localized diseases; and the ability of reproduction continuesto decline in generations, there fore the availability of parental cattle should be maintained continuously. This crossbreed had producedsome new breeds, among others Simpo (Simmental x PO), Limpo (Limousin x PO), Simbal (Simmental x Bali cattle), Limbal(Limousin x Bali cattle), and Madrasin or Limad (Limousin x Madura cattle). Male offsprings were sterile, while female offsprings hadlower reproductive capacity than of the parentâ€™s. This lead to uncertainty over the guarantee of meeting the needs of protein (meat andmilk) of Indonesian in the future, thus there was a need of regulation. On the other hand, in the grasslands of North Australia, thebreeder had produced an eminent cattle breeds, namely Australian Commercial Cattle (ACC), from uncontrolled crossbreeds betweendifferent breedsof taurine and zebu cattle in the pasture, therefore this concerns ignored.

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DNA polymorphism at locus-2 of growth hormone gene of Madura cattle

Cited by 5 publications

References 0 publications

Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method

Identification of growth hormone gene variation in exon region at Indonesian Local Cattle based on PCR-SSCP method

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

Review: Genetic diversity of local and exotic cattle and their crossbreeding impact on the quality of Indonesian cattle

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