DNA vaccination with VP2 gene fragment confers protection against Infectious Bursal Disease Virus in Chickens

Pradhan, Satya Narayan; Prince, Prabhu Rajaiah; Madhumathi, Jayaprakasam; Arunkumar, Chakkaravarthy; Roy, Parimal; Narayanan, R. B.; Antony, Usha

doi:10.1016/j.vetmic.2014.02.019

Cited by 17 publications

(7 citation statements)

References 34 publications

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“…Clostridium perfringens [32]). While we have shown that the Eimeria expression system can be used to stimulate host immune recognition of foreign antigens, the levels of humoral response generated are likely to be insufficient to guarantee protection against challenge [33]. Prior to conducting an in vivo challenge the consistency and magnitude of immune response stimulated by transgenic Eimeria vaccination should be improved.…”

Section: Discussionmentioning

confidence: 99%

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

et al. 2016

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BackgroundEimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens. Vaccination against chicken coccidiosis is effective using wild-type or attenuated live parasite lines. The development of protocols to express foreign proteins in Eimeria species has opened up the possibility of using Eimeria live vaccines to deliver heterologous antigens and function as multivalent vaccine vectors that could protect chickens against a range of pathogens.ResultsIn this study, genetic complementation was used to express immunoprotective virus antigens in Eimeria tenella. Infectious bursal disease virus (IBDV) causes Gumboro, an immunosuppressive disease that affects productivity and can interfere with the efficacy of poultry vaccination programmes. Infectious laryngotracheitis virus (ILTV) causes a highly transmissible respiratory disease for which strong cellular immunity and antibody responses are required for effective vaccination. Genes encoding the VP2 protein from a very virulent strain of IBDV (vvVP2) and glycoprotein I from ILTV (gI) were cloned downstream of 5’Et-Actin or 5’Et-TIF promoter regions in plasmids that also contained a mCitrine fluorescent reporter cassette under control of the 5’Et-MIC1 promoter. The plasmids were introduced by nucleofection into E. tenella sporozoites, which were then used to infect chickens. Progeny oocysts were sorted by FACS and passaged several times in vivo until the proportion of fluorescent parasites in each transgenic population reached ~20 % and the number of transgene copies per parasite genome decreased to < 10. All populations were found to transcribe and express the transgene and induced the generation of low titre, transgene-specific antibodies when used to immunise chickens.ConclusionsE. tenella can express antigens of other poultry pathogens that are successfully recognised by the chicken immune system. Nonetheless, further work has to be done in order to improve the levels of expression for its future use as a multivalent vaccine vector.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1756-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

et al. 2016

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“…DNA vaccines have great potential as an alternative capable of inducing protective immune responses against a variety of infectious diseases. Several studies have been conducted for the development of a DNA vaccine encoding the VP2 gene of IBDV, with variable protection results (Pradhan et al, 2014). However, a DNA vaccine encoding the VP2 gene alone has limited potency and results in only partial protection.…”

Section: Immunosuppression Interventionsmentioning

confidence: 99%

Interaction of infectious bursal disease virus with the immune system of poultry

Rehman

Chen

Umar

et al. 2016

World's Poultry Science Journal

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Immune dysfunction can be either at the humoral or cellular levels and is mediated by a myriad of factors including virus-induced immunosuppression. Infectious bursal disease virus (IBDV) affects domesticated poultry and causing health problems mainly due to prolonged immunosuppression. Destruction of the immunoglobulin-producing cells is the principal cause of IBDV-induced immunosuppression, which leads to significant impairment of the primary antibody responses. Due to these effects, IBDV infection not only increases the susceptibility of poultry to other viral infections but predisposes the host to several other bacteria of variable pathologies. The IBDV-induced immunesuppression is well-known phenomenon, however, recently there have been significant advancements in understanding the molecular mechanisms of this immune-suppression. This review discuss current updates regarding the immunotoxic and immunosuppressive nature of IBDV in the poultry and highlights areas requiring future research attentions that may help to establish foundations for effective and improved vaccines against IBDV.

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“…IBD virus consists of two segments includes A constitutes VP2, VP3 and VP4 while the smaller segment B encodes only VP1. IBD is highly infectious disease and the only control is vaccination [12,13]. VP2 segment of the infectious bursal disease virus considered as the most immunogenic region and could be used in the development of vaccine for the effective control of infectious bursal disease in poultry birds to eliminate the major economic losses.…”

Section: Discussionmentioning

confidence: 99%

Infectious bursal disease virus cloning and structural protein (VP2) expression in Escherichia coli

Sajid

Khan

2020

PAB

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Immunosuppressive diseases like infectious bursal disease (IBD) are serious threat to the poultry meat industry. IBD virus consists of two genomic segments in which segment A encodes all structural and non-structural proteins (VP2, VP3, VP4) and segment B encodes RNA dependent RNA polymerase VP1. In IBD the host immune response is due to the VP2 capsid protein of the virus that leads to the immunosuppression of the birds. In the current research the partial VP2 clones were developed using cRNA based reversed genetic system to understand the molecular determinants of infectious bursal disease virus for pathogenic phenotype and virulence. For the expression analysis, the cloning primers were designed for amplification containing NdeI and EcoRI restriction sites for the attachment of restriction enzymes. VP2 of the IBDV was expressed in E. coli cells with the utilization of pET28a expression vector. Expression analysis of bacterial lysate on SDS-PAGE after IPTG induction of 5 hours with 1mM concentration, 30 KDa VP2 protein band was observed. This study suggests the use of recombinant VP2 (rVP2) as a subunit vaccine.

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DNA vaccination with VP2 gene fragment confers protection against Infectious Bursal Disease Virus in Chickens

Cited by 17 publications

References 34 publications

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Interaction of infectious bursal disease virus with the immune system of poultry

Infectious bursal disease virus cloning and structural protein (VP2) expression in Escherichia coli

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