Docosahexaenoic acid production by a novel high yielding strain of Thraustochytrium sp. of Indian origin: Isolation and bioprocess optimization studies

Chandrasekaran, Kabilan; Roy, Rony K.; Chadha, Anju

doi:10.1016/j.algal.2018.03.011

Cited by 26 publications

(29 citation statements)

References 31 publications

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“…The present study recorded 43.68% saturated fatty acid, 4.90% monounsaturated fatty acid, and 51.42% polyunsaturated fatty acids in Thraustochytrium kinnei, in addition to better fatty acid profile in terms of essential fatty acids such as alpha-linolenic acid (ALA) (0.33%), eicosapentaenoic acid (EPA) (7.76%), docosahexaenoic acid (DHA) (39.16%), arachidonic acid (1.67%) and docosapentaenoic acid (DPA) (2.09%) (Table 5). Thus, thraustochytrids are promising for the production of essential fatty acids, in accordance with earlier reports [1,44].…”

Section: Discussionsupporting

confidence: 91%

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Kalidasan

Nabikhan

Gomathi

et al. 2021

JMSE

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This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

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Section: Discussionsupporting

confidence: 91%

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Kalidasan

Nabikhan

Gomathi

et al. 2021

JMSE

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“…Microbial oil, being produced in a controlled environment, is one of the current topics of massive research because it has many advantages compared to fish oil [6,7]. Marine heterotrophic protists, largely known as marine microalgae, especially Aurantiochytrium spp., have been shown to be excellent DHA producers.…”

Section: Introductionmentioning

confidence: 99%

Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

et al. 2020

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In the present study, the impact of eight phytohormones from six different classes on the growth, lipid and docosahexaenoic acid (DHA) biosynthetic capacity of Aurantiochytrium sp. SW1 (SW1) was evaluated. Kinetin (KIN), jasmonic acid (JA) and gibberellic acid (GA) significantly enhanced the growth and DHA production of SW1 by 16%–28% and 66%–84% in comparison to the control, respectively. The synergistic effect of these three phytohormones, evaluated by the response surface methodology (RSM), showed that a combination of 3.6 mg/L GA, 2.0 mg/L KIN and 20.0 mg/L JA further increased the growth and DHA production of SW1 by 16% to 28% and 22% to 36%, respectively, in comparison to the individual supplementation. The synergistic effect of these phytohormones was also shown to be time-dependent, where feeding at 24 h of cultivation led to 15%, 26% and 35% further increments in the biomass, lipid and DHA production in comparison to that of 0 h, respectively. The determination of stress markers, antioxidant enzymes and key enzymes involved in fatty acid biosynthesis aided to elucidate the potential mechanism underlying the improvement of growth and DHA production by SW1 at various times of feeding. Supplementation with the phytohormones at 24 h exhibited the maximum impact on reducing the level of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as augmented the antioxidants (superoxide dismutase and catalase) and key metabolic enzymes involved in lipogenesis (malic, glucose-6-phosphate dehydrogenase and ATP-citrate lyase) in comparison to the control and other time points. This study signifies the potential application of phytohormones for improving the growth, lipid and DHA production in Aurantiochytrium spp.

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“…Thraustochytrium is a marine protist that can produce a relatively large amount of VLCPUFAs, mostly DHA. Therefore, Thraustochytrium and other related species have attracted much scientific and industrial interest in the production of DHA for commercial uses through fermentation and metabolic engineering (Patil and Gogate, 2015; Chandrasekaran et al, 2018; Chen and Yang, 2018; Sun et al, 2018). Our previous work indicates that DHA is solely synthesized by the anaerobic pathway using a polyketide synthase-like PUFA synthase comprising three large subunits each with multiple functional domains, whereas the aerobic pathway employing desaturases and elongases is incomplete in Thraustochytrium (Meesapyodsuk and Qiu, 2016; Zhao and Qiu, 2018).…”

Section: Introductionmentioning

confidence: 99%

Very Long Chain Polyunsaturated Fatty Acids Accumulated in Triacylglycerol Are Channeled From Phosphatidylcholine in Thraustochytrium

Zhao

Qiu

2019

Front. Microbiol.

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Thraustochytrium is a marine protist that can accumulate a large amount of very long chain polyunsaturated fatty acids (VLCPUFA) in triacylglycerols (TAG). How these freshly synthesized VLCPUFAs are channeled into TAG remains unknown. In this study, the glycerolipid profile of Thraustochytrium at log and stationary growth stages was first analyzed by lipidomic tools, and then 14 C-acetate and 14 C-glycerol were used to trace the flux of fatty acids and backbone in glycerolipids. Lipidomic analysis showed that VLCPUFAs were mostly allocated to phosphatidylcholine (PC) and TAG. PC possessed a relatively stable profile of VLCPUFAs, whereas TAG carrying VLCPUFAs were significantly increased at the stationary phase. 14 C-acetate labeled VLCPUFAs were predominately incorporated into PC initially but were mostly found in TAG at later time of labeling. Positional analysis showed that PC had either one VLCPUFA at its sn -2 position (PC1) or two VLCPUFAs (PC2), while TAG incorporated VLCPUFAs almost exclusively at the sn -2 position. Similarly, 14 C-glycerol was more efficiently incorporated into PC1 than TAG initially but was mostly found in TAG at later time of labeling, and diacylglycerol and PC1 shared a similar incorporation pattern. These results indicate that VLCPUFAs in TAG are mainly channeled from PC likely through diacylglycerol as the intermediate.

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Docosahexaenoic acid production by a novel high yielding strain of Thraustochytrium sp. of Indian origin: Isolation and bioprocess optimization studies

Cited by 26 publications

References 31 publications

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

Very Long Chain Polyunsaturated Fatty Acids Accumulated in Triacylglycerol Are Channeled From Phosphatidylcholine in Thraustochytrium

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