Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p

Wang, Lingna; Lewis, Marc S.; Johnson, Arlen

doi:10.1261/rna.2060405

Cited by 62 publications

(51 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The core of the Ski complex is formed by the interaction of Ski2 with a large TPR protein (Ski3) and a small WD40 protein (Ski8). Yeast two-hybrid and coimmunoprecipitation data suggest that the N-terminal region of Ski2 mediates binding to Ski3, which in turn interacts with Ski8 (Wang et al 2005). In line with these data, coexpression of f.l.…”

Section: The Ski2 Insertion Is Not Required To Form the Ski Complexsupporting

confidence: 62%

“…S. cerevisiae ortholog (residues 1-1287) from insect cells. Ski2 is predicted to have a low-complexity N-terminal region that has previously been shown to interact with Ski3 by yeast two-hybrid and coimmunoprecipitation analyses (Wang et al 2005). Limited proteolysis of f.l.…”

Section: Resultsmentioning

confidence: 99%

“…4A). We next tested whether the insertion of Ski2 is required to bind Ski7, an outer-layer protein of the Ski complex that mediates the interaction with the exosome (Araki et al 2001;Wang et al 2005). In in-vitro pull-down experiments with purified proteins, f.l.…”

Section: The Ski2 Insertion Is Not Required To Form the Ski Complexmentioning

confidence: 99%

See 2 more Smart Citations

The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome

Halbach

Rode

Conti

2011

RNA

View full text Add to dashboard Cite

Ski2 is a cytoplasmic RNA helicase that functions together with the exosome in the turnover and quality control of mRNAs. Ski2 is conserved in eukaryotes and is related to the helicase Mtr4, a cofactor of the nuclear exosome involved in the processing and quality control of a variety of structured RNAs. We have determined the 2.4 Å resolution crystal structure of the 113 kDa helicase region of Saccharomyces cerevisiae Ski2. The structure shows that Ski2 has an overall architecture similar to that of Mtr4, with a core DExH region and an extended insertion domain. The insertion is not required for the formation of the Ski2-Ski3-Ski8 complex, but is instead an RNA-binding domain. While this is reminiscent of the Mtr4 insertion, there are specific structural and biochemical differences between the two helicases. The insertion of yeast Mtr4 consists of a b-barrel domain that is flexibly attached to a helical stalk, contains a KOW signature motif, and binds in vitro-transcribed tRNA iMet , but not singlestranded RNA. The b-barrel domain of yeast Ski2 does not contain a KOW motif and is tightly packed against the helical stalk, forming a single structural unit maintained by a zinc-binding site. Biochemically, the Ski2 insertion has broad substrate specificity, binding both single-stranded and double-stranded RNAs. We speculate that the Ski2 and Mtr4 insertion domains have evolved with different properties tailored to the type of transcripts that are the substrates of the cytoplasmic and nuclear exosome.

show abstract

Section: The Ski2 Insertion Is Not Required To Form the Ski Complexsupporting

confidence: 62%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome

Halbach

Rode

Conti

2011

RNA

View full text Add to dashboard Cite

show abstract

“…Ski8 forms a cytoplasmic complex with Ski2 and Ski3 Wang et al 2005) that is required for inhibition of translation of nonpolyadenylated RNA and for 3′→5′ exonucleolytic degradation of RNA (Masison et al 1995;Jacobs Anderson and Parker 1998;van Hoof et al 2000;Araki et al 2001). During meiosis, however, Ski8 relocalizes from the cytoplasm to the nucleus and associates with meiotic chromosomes, dependent on Spo11 in both S. cerevisiae and S. macrospora Arora et al 2004).…”

Section: Ski8-ski8mentioning

confidence: 99%

Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis

Keeney

Genome Dynamics and Stability

311

359

View full text Add to dashboard Cite

Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks made by the Spo11 protein, a relative of archaeal topoisomerase VI. This review summarizes recent studies that provide insight to the mechanism of DNA cleavage by Spo11, functional interactions of Spo11 with other proteins required for break formation, mechanisms that control the timing of recombination initiation, and evolutionary conservation and divergence of these processes.

show abstract

“…To confirm the diagnosis of Rotavirus infection, we used two tests and are always recommended for surveillance. The ELISA is carried out in our reference or regional site with a quality control carried out by INRB [11][12][13]. The PCR for genotyping is conducted at the National Reference Laboratory (NRL) of INRB, Kinshasa/DRC, at the Regional Reference Laboratory (RRL) of Mendusa University, and at the CDC Global Reference Laboratory (GRL) Atlanta, USA.…”

Section: Support Strategiesmentioning

confidence: 99%

Monitoring Sentinel of Diarrhea: A Rotavirus in the Site of Hospital Complex Pediatrique of Kingasani II: 2009-2014

Mukwela¹,

Kitambala²,

I³

et al. 2017

J Gastrointest Dig Syst

View full text Add to dashboard Cite

After paludism, respiratory infections, and meningitis, the severe diarrhoea belongs to the major causes of the problems of public health in the whole world. According to current statistics' of WHO, this disease cause at least 483 000 deaths of children from zero to 59 months per annum in the world. The children whose above mentioned age, are victims permanant of this very fatal disease. Let us announce by here that the vaccine against the gastro entérite with rotavirus is already operational in certain developed countries whose principal objective remains that to reduce its incidence. In addition, no one is not unaware of that the diarrhoea with rotavirus remains one of the concerns paramount among the problems of public health in the countries under developed and sends development; they is the cases in particular of the Latin America and certain sub-Saharan countries of Africa. At strong present remains to draw the alarm bell near the authorities politico medical and administrative on all the levels in order to make a plea with close to the backers for establishment again vaccine with rotavirus especially in the countries with strong incidence of this cruel disease (case of the RDC). This would reduce the incidence. By way of recall, it ya place to announce by here that it is since 2009 that the ministry for the public health of the RDC, via its program widened of vaccination (PEV) with the support of the certain partners and financial backers of the bottom had made the installation of a study on the incidence of this disease.

show abstract

Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p

Cited by 62 publications

References 34 publications

The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome

The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome

Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis

Monitoring Sentinel of Diarrhea: A Rotavirus in the Site of Hospital Complex Pediatrique of Kingasani II: 2009-2014

Contact Info

Product

Resources

About