Dopamine-induced epithelial K+ and Na+ movements in the salivary ducts of Periplaneta americana

Lang, Ingo

doi:10.1016/s0022-1910(00)00134-7

Cited by 27 publications

(36 citation statements)

References 25 publications

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“…This level fits well to electrophysiological data of (22 ± 19.3) mM. 39 About 1 min after dopamine application, a significant [Na + ] i increase to (34.1 ± 4.1) mM (N = 6) could be observed (P = 0.0313), which was totally reversible after dopamine washout (Fig. 6A).…”

Section: Physiological Experiments In Salivary Duct and Acinar Cellssupporting

confidence: 88%

“…So, a reversible dopamineinduced [Na + ] i rise could be measured in duct cells quantitatively with Na + -sensitive microelectrodes. 39 This effect could be confirmed qualitatively using 2P-excited steady-state fluorescence recordings. 17 In addition, that study had been also demonstrated for the first time a dopamine-induced [Na + ] i increase in the acinar peripheral cells.…”

Section: Physiological Experiments In Salivary Duct and Acinar Cellsmentioning

confidence: 74%

“…17 According to this, inhibition of the NKCC can indirectly influence the dopamine-induced effects in the duct cells as shown electrophysiologically. 39 Indeed, bath application of 1 µM dopamine together with 50 µM bumetanide, a well-known NKCC inhibitor, 40 resulted in a complete suppression of the dopamine-induced [Na + ] i rise in duct cells (Fig. 7).…”

Section: Physiological Experiments In Salivary Duct and Acinar Cellsmentioning

confidence: 92%

“…The dopamine-induced [Na + ] i rise recorded here is markedly lower than that of an electrophysiological study, in which a huge [Na + ] i rise up to 92 mM was recorded. 39 It should be noted that these [Na + ] i changes were recorded with two hardly comparable techniques, because the ion-selective microelectrodes represent an invasive point measurement method and 2P-FLIM is a large-scale non-invasive method. Indeed, this could have an impact on the measured [Na + ] i .…”

Section: Physiological Experiments In Salivary Duct and Acinar Cellsmentioning

confidence: 99%

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ANG-2 for quantitative Na+ determination in living cells by time-resolved fluorescence microscopy

Roder

Hille

2014

Photochem Photobiol Sci

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U n i v e r s i t ä t P o t s d a mPostprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe ; 184 Phillip Roder and Carsten Hille* Sodium ions (Na + ) play an important role in a plethora of cellular processes, which are complex and partly still unexplored. For the investigation of these processes and quantification of intracellular Na + concentrations ([Na + ] i ), two-photon coupled fluorescence lifetime imaging microscopy (2P-FLIM) was performed in the salivary glands of the cockroach Periplaneta americana. For this, the novel Na + -sensitive fluorescent dye Asante NaTRIUM Green-2 (ANG-2) was evaluated, both in vitro and in situ. In this context, absorption coefficients, fluorescence quantum yields and 2P action cross-sections were determined for the first time. ANG-2 was 2P-excitable over a broad spectral range and displayed fluorescence in the visible spectral range. Although the fluorescence decay behaviour of ANG-2 was triexponential in vitro, its analysis indicates a Na + -sensitivity appropriate for recordings in living cells. The Na + -sensitivity was reduced in situ, but the biexponential fluorescence decay behaviour could be successfully analysed in terms of quantitative [Na + ] i recordings. Thus, physiological 2P-FLIM measurements revealed a dopamineinduced [Na + ] i rise in cockroach salivary gland cells, which was dependent on a Na + -K + -2Cl − cotransporter (NKCC) activity. It was concluded that ANG-2 is a promising new sodium indicator applicable for diverse biological systems.

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Section: Physiological Experiments In Salivary Duct and Acinar Cellssupporting

confidence: 88%

Section: Physiological Experiments In Salivary Duct and Acinar Cellsmentioning

confidence: 74%

Section: Physiological Experiments In Salivary Duct and Acinar Cellsmentioning

confidence: 92%

Section: Physiological Experiments In Salivary Duct and Acinar Cellsmentioning

confidence: 99%

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ANG-2 for quantitative Na+ determination in living cells by time-resolved fluorescence microscopy

Roder

Hille

2014

Photochem Photobiol Sci

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“…Primary saliva secretion resulting from transepithelial NaCl and water transport through the peripheral cells is driven by an apical Na + /K + -ATPase (Gupta and Hall, 1983;Just and Walz, 1994b). A basolateral Na + /K + -ATPase and an apical vacuolar-type proton pump (V-H + -ATPase) in the duct epithelial cells are thought to be important for saliva modification (Lang and Walz, 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of neurotransmitter-induced electrolyte transport in cockroach salivary glands by intracellular Ca2+, Na+ and pH measurements in duct cells

Hille

2008

Journal of Experimental Biology

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The aminergic control of cockroach salivary glands

Baumann

Krach

Baumann

et al. 2006

Arch Insect Biochem Physiol

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The acinar salivary glands of cockroaches receive a dual innervation from the subesophageal ganglion and the stomatogastric nervous system. Acinar cells are surrounded by a plexus of dopaminergic and serotonergic varicose fibers. In addition, serotonergic terminals lie deep in the extracellular spaces between acinar cells. Excitation-secretion coupling in cockroach salivary glands is stimulated by both dopamine and serotonin. These monoamines cause increases in the intracellular concentrations of cAMP and Ca(2+). Stimulation of the glands by serotonin results in the production of a protein-rich saliva, whereas stimulation by dopamine results in saliva that is protein-free. Thus, two elementary secretory processes, namely electrolyte/water secretion and protein secretion, are triggered by different aminergic transmitters. Because of its simplicity and experimental accessibility, cockroach salivary glands have been used extensively as a model system to study the cellular actions of biogenic amines and to examine the pharmacological properties of biogenic amine receptors. In this review, we summarize current knowledge concerning the aminergic control of cockroach salivary glands and discuss our efforts to characterize Periplaneta biogenic amine receptors molecularly.

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Dopamine-induced epithelial K+ and Na+ movements in the salivary ducts of Periplaneta americana

Cited by 27 publications

References 25 publications

ANG-2 for quantitative Na+ determination in living cells by time-resolved fluorescence microscopy

ANG-2 for quantitative Na+ determination in living cells by time-resolved fluorescence microscopy

Characterisation of neurotransmitter-induced electrolyte transport in cockroach salivary glands by intracellular Ca2+, Na+ and pH measurements in duct cells

The aminergic control of cockroach salivary glands

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