Dose dependence of radiation-induced micronuclei in cytokinesis-blocked human lymphocytes

Silva, Maria João; Carothers, Andrew D.; Dias, Anabela; Luís, José; Piper, Jim; Boavida, Maria Guida

doi:10.1016/0165-1218(94)00019-0

Cited by 28 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1(a), the obtained dose-response curves, obtained at all depths, showed the best fitness with the linear-quadratic models. The variations of the micronuclei response as a function of absorbed dose through a quadratic equation are comparable with the studies of Prosser et al 35 and Silva et al 36 Likewise, they obtained a quadratic equation for the dose-response curve in the micronuclei method.…”

Section: Resultssupporting

confidence: 88%

Dependence of micronuclei assay on the depth of absorbed dose

Abtahi

Aghamiri

Yadolahi

et al. 2017

Reports of Practical Oncology & Radiotherapy

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 88%

Dependence of micronuclei assay on the depth of absorbed dose

Abtahi

Aghamiri

Yadolahi

et al. 2017

Reports of Practical Oncology & Radiotherapy

View full text Add to dashboard Cite

show abstract

“…These findings are similar to former studies performed by Silva et al [17], Koksal G et al [18] and Gantonberg HW et al [19]. Our results have released that radiosensitivity index is elevated in left handed women compared to right handed women and also it's evident that difference of the mean MN frequency in left-handers compared to right-handers is higher.…”

Section: Discussionsupporting

confidence: 93%

The study of radiosensitivity in left handed compared to right handed healthy women

et al. 2012

View full text Add to dashboard Cite

BackgroundRadiosensitivity is an inheriting trait that mainly depends on genetic factors. it is well known in similar dose of ionizing radiation and identical biological characteristics 9–10 percent of normal population have higher radiation response. Some reports indicate that distribution of breast cancer, immune diseases including autoimmune diseases as example lupus, Myasthenia Gravies and even the rate of allergy are more frequent in left handed individuals compared to right handed individuals. The main goal of the present study is determination of radiosensitivity in left handed compared to right handed in healthy women by cytokinesis blocked micronuclei [CBMN] assay.5 ml peripheral fresh blood sample was taken from 100 healthy women including 60 right handed and 40 left handed. The age of participants was between 20–25 old years and they had been matched by sex. After blood sampling, blood samples were divided to 2 groups including irradiated and non-irradiated lymphocytes that irradiated lymphocytes were exposed to 2 Gy CO-60 Gama rays source then chromosomal aberrations was analyzed by CBMN [Cytokinesis Blocked Micronuclei Assay].ResultsOur results have shown radiosensitivity index [RI] in left-handers compared to right-handers is higher. Furthermore, the mean MN frequency is elevated in irradiated lymphocytes of left-handers in comparison with right-handers.ConclusionOur results from CBMN assay have shown radiosensitivity in the left handed is higher than right handed women but more attempts need to prove this hypothesis.

show abstract

“…The slides were post-fixed for 10 min in a solution of formaldehyde/MgCl 2 (1% formaldehyde/50 mM MgCl 2 in PBS). The slides after denaturation (2X SSC at 70°C for 20 min and after cooling to ambient temperature 1 min in 0.07 N NaOH) were dehydrated in graded series of ethanol (30,70, 90 and 100%) and air dried. The M-FISH probe mixture was denatured separately by incubation at 75°C for 5 min followed by incubation at 37°C for 30 min to allow the annealing of repetitive DNA sequences.…”

Section: Cell Culture and Irradiationmentioning

confidence: 99%

Multicolour FISH analysis of ionising radiation induced micronucleus formation in human lymphocytes

et al. 2014

View full text Add to dashboard Cite

Micronucleation of chromosomal DNA is an effective indicator of DNA damage and micronucleus (MN) analysis is a valuable tool for radiation biodosimetry studies. To gain a comprehensive knowledge of micronucleation process after ionising radiation (IR) exposure, whole genome-wide chromosome analysis is desirable. With this objective, multicolour fluorescence in situ hybridization (M-FISH) technique was utilised in the present study to characterise the chromosome content of spontaneous and IR-induced micronuclei in three human donors. M-FISH analysis revealed a radiation dose-dependant increase in the number of micronuclei with multi-chromosome material above 2 Gy and as many as 3-6 multicolour signals were detected in micronuclei after high γ-rays radiation doses (5-10 Gy). Involvement of each human chromosome material was more frequently detected in multicoloured micronuclei than in single-coloured micronuclei at high radiation doses (>2 Gy). Observation of dose-dependant increase in the MN frequency with multi-chromosome material may be due to misrepair of DNA double-strand breaks involving multiple chromosomes leading to asymmetric dicentric or ring chromosomes and acentric fragments. Chromosomes belonging to groups A (1, 2 and 3) and B (4 and 5) were frequently detected in 35-45% of the total micronuclei either as single entities or in combination with other chromosomes. Among the A and B groups, chromosome 1 material was consistently detected at high MN frequencies after radiation exposure in all the donors. Additionally, chromosomes 13 and 19 were more frequently observed in micronuclei than the expected frequency based on DNA content. Our whole genome approach utilising the M-FISH technique revealed that MN formation at high radiation doses might be complex involving multiple chromosome fragments. Understanding the fate and biological consequences of these multi-chromosome-containing micronuclei may provide key molecular insights for some aspects of IR-induced genomic instability and cancer development processes.

show abstract

Dose dependence of radiation-induced micronuclei in cytokinesis-blocked human lymphocytes

Cited by 28 publications

References 28 publications

Dependence of micronuclei assay on the depth of absorbed dose

Dependence of micronuclei assay on the depth of absorbed dose

The study of radiosensitivity in left handed compared to right handed healthy women

Multicolour FISH analysis of ionising radiation induced micronucleus formation in human lymphocytes

Contact Info

Product

Resources

About