Dose-Response Curve to Secretin on Gastric Mucus Secretion in Conscious Cats

Abstract

The effect of secretin on gastric mucus was studied simultaneously with acid and pepsin secretions in three conscious cats with gastric fistula and Heidenhain pouch. The glycoprotein fraction of mucus was estimated by fucose and galactose concentration and the polysaccharide fraction by uronic acid concentration in homogenized gastricjuice. Secretin produced stimulation of both fractions which persisted during continuous infusion and was proportional to the doses. However, this action was greater on the polysaccharide than on the glycoprotein fraction and the variations of pepsin and uronic acids were strongly correlated. Secretin stimulates mucus secretion when no parietal stimulation is induced.

Effect of Secretin on Mucinous Secretion by the Isolated Canine Stomach Perfused Extracorporeally

“…In agreement with previous studies, we found that secretin induced an increase in the output of major carbohydrate components, represent ing glycoprotein fraction of gastric mucus and also in the output of uronic acid, considered a representative of the polysaccharide fraction of gastric mucus (9). This study agrees with our previous experiment, concerned with the com position of gastric mucus, secreted by the iso lated mammalian stomach perfused extracorporeally (11).…”

“…Secretin was found previously to stimulate gastric mucus secretion in cats (8,9) and man (7), and in Brunner canine pouches (25). Vague (9) estimated glycoprotein fractions of mucus by fucose and galactose concentration and the polysaccharide fraction by uronic acid concen tration in homogenized gastric juice.…”

“…Vague (9) estimated glycoprotein fractions of mucus by fucose and galactose concentration and the polysaccharide fraction by uronic acid concen tration in homogenized gastric juice. Secretin produced stimulation of both glycoprotein and polysaccharide fractions (8,9). André et al (7) found, in man, that secretin induced a sustained increase in the output of fucose, galactose, total hexosamines, uronic acid and sulfate.…”

“…Mucin produc tion by gastric mucosa has been investigated in clinical conditions and in experimental animals, in vivo (1 -10) and under ex vivo (11,12) conditions. Gastric mucus secretion can be stimulated by secretin, in man (7) and in cats (8,9). Secretin is a known inhibitor of gastric acid secretion and an effective stimulant of gastric pepsin, both in vivo (13) and under ex vivo conditions (14).…”

Effects of Sodium Fluoride on Water and Acid Secretion, Soluble Mucus and Adherent Mucus of the Rat Stomach

“…Estimation of luminal mucus in gastric washings has been used extensively as an index of secretion. For example, pentagastrin, histamine and prostaglandins have been reported to increase luminal glycoproteins (25)(26)(27)(28)(29). However, various phenomena may induce variation in soluble mucus -a shift of adherent glycoproteins to soluble mucus, an increased rate of mucus secretion of adherent glycoproteins or an increased breakdown of mucus by excessive mucolytic activity of pepsin.…”