Double overexpression of <scp>DREB</scp> and <scp>PIF</scp> transcription factors improves drought stress tolerance and cell elongation in transgenic plants

Kudo, Madoka; Kidokoro, Satoshi; Yoshida, Takuya; Mizoi, Junya; Todaka, Daisuke; Fernie, Alisdair R.; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

doi:10.1111/pbi.12644

Cited by 147 publications

(98 citation statements)

References 61 publications

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“…DREB genes from several species have been reported to be functionally involved in the regulation of plant responses to drought. These include Arabidopsis DREB1A and DREB2A (Kudo et al ., ; Yamaguchi‐Shinozaki and Shinozaki, ), soya bean GmDREB2 (Chen et al ., ) and GmERF3 (Zhang et al ., ), tomato JERF1 (Zhang et al ., ) and SIERF5 (Pan et al ., ) and apple MsDREB6.2 (Liao et al ., ), among others.…”

Section: Discussionmentioning

confidence: 99%

NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato

Thirumalaikumar

Devkar

Mehterov

et al. 2017

Plant Biotechnology Journal

253

116

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SummaryWater deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell‐damaging reactive oxygen species and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus‐induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2O2) levels and a decrease in the expression of various drought‐responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2 and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress‐related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato.

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Section: Discussionmentioning

confidence: 99%

NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato

Thirumalaikumar

Devkar

Mehterov

et al. 2017

Plant Biotechnology Journal

253

116

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“…Alternatively, gene-stacking approaches have proven fruitful in overcoming growth inhibition using positive regulators of drought responses such as DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN1A (DREB1A). Overexpression of DREB1A led to increased drought tolerance and growth inhibition, but the growth limitation could be overcome by simultaneous overexpression of DREB1A together with the rice homolog of PIF4 (OsPIL1) in Arabidopsis (Kudo et al, 2016). Along these lines, an elegant study demonstrated that overexpressing the BR receptor gene BRL3 increased plant survival during drought without the growth penalty observed in BR mutants such as the loss-of-function mutant bri1 (Fàbregas et al, 2018).…”

Section: Tissue-specific Modulation Of the Br Pathway Allows For Incrmentioning

confidence: 99%

Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses

et al. 2019

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Brassinosteroids (BRs) are a group of polyhydroxylated plant steroid hormones that are crucial for many aspects of a plant's life. BRs were originally characterized for their function in cell elongation, but it is becoming clear that they play major roles in plant growth, development, and responses to several stresses such as extreme temperatures and drought. A BR signaling pathway from cell surface receptors to central transcription factors has been well characterized. Here, we summarize recent progress toward understanding the BR pathway, including BR perception and the molecular mechanisms of BR signaling. Next, we discuss the roles of BRs in development and stress responses. Finally, we show how knowledge of the BR pathway is being applied to manipulate the growth and stress responses of crops. These studies highlight the complex regulation of BR signaling, multiple points of crosstalk between BRs and other hormones or stress responses, and the finely tuned spatiotemporal regulation of BR signaling.

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“…Although the regulatory functions of the PIFs have been widely explored in light responses, seed germination and hormone regulation, the roles of PIFs in the drought stress response remain elusive (Leivar and Quail, 2011;Oh et al, 2009). The rice PIF-like protein OsPIL1 is down-regulated under drought stress conditions (Todaka et al, 2012), and overexpression of both DREB1A and OsPIL1 enhances drought tolerance in Arabidopsis (Kudo et al, 2016), while ZmPIF3 regulates plant responses to drought stresses (Gao et al, 2015). Thus, PIFs likely play complicated roles under drought stress.…”

Section: Introductionmentioning

confidence: 99%

A maize phytochrome‐interacting factors protein ZmPIF1 enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa

Gao

Zhang

et al. 2018

Plant Biotechnology Journal

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SummaryPhytochrome‐interacting factors (PIFs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. Here, we cloned and characterized a maize (Zea mays) PIF transcription factor, ZmPIF1. The expression level of ZmPIF1 was significantly induced by independent drought and abscisic acid (ABA) treatments. The ZmPIF1 transgenic rice and Arabidopsis displayed water saving and drought resistance, which were associated with reduced a stomatal aperture and transpiration rate. Moreover, the ZmPIF1 transgenic rice were hypersensitive to exogenous ABA, while the endogenous ABA level was not significantly changed, suggesting that ZmPIF1 was a positive regulator of the ABA signalling pathway. Digital gene expression (DGE) results further indicated that ZmPIF1 participated in ABA signalling pathway and regulated the stomatal aperture in rice. In addition, grain yield and agronomic traits analysis over 4 years showed that ZmPIF1 was able to increase the grain yield through an increase in tiller and panicle numbers in transgenic rice. Overall, ZmPIF1 plays an important role in the ABA‐mediated regulation of stomatal closure to control water loss. ZmPIF1 can enhance water saving and drought resistance and improve the crop yield in rice, illustrating the capacity of ZmPIF1 for crop improvement.

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Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants

Cited by 147 publications

References 61 publications

NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato

NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato

Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses

A maize phytochrome‐interacting factors protein ZmPIF1 enhances drought tolerance by inducing stomatal closure and improves grain yield in Oryza sativa

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