Double-stranded RNA and isometric virus-like particles in the entomopathogenic fungus Metarhizium anisopliae

Bogo, Maurı́cio Reis; Queiroz, Marisa Vieira de; Silva, D.M.; Giménez, M.; Azevedo, João Lúcio de; Schrank, Augusto

doi:10.1016/s0953-7562(96)80079-7

Cited by 25 publications

(5 citation statements)

References 13 publications

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“…From the seminal discovery in the C. neoformans until now, fungal EVs were identified in at least eight additional fungal species [9–11,14]. The hypothesis that EVs by are produced by additional fungal species is supported by different reports describing vesicle-like structures as components of regular physiologic events [43] or products of viral infections [44]. …”

Section: Fungal Evsmentioning

confidence: 99%

Where Do They Come from and Where Do They Go: Candidates for Regulating Extracellular Vesicle Formation in Fungi

Oliveira

Rizzo

Joffe

et al. 2013

IJMS

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In the past few years, extracellular vesicles (EVs) from at least eight fungal species were characterized. EV proteome in four fungal species indicated putative biogenesis pathways and suggested interesting similarities with mammalian exosomes. Moreover, as observed for mammalian exosomes, fungal EVs were demonstrated to be immunologically active. Here we review the seminal and most recent findings related to the production of EVs by fungi. Based on the current literature about secretion of fungal molecules and biogenesis of EVs in eukaryotes, we focus our discussion on a list of cellular proteins with the potential to regulate vesicle biogenesis in the fungi.

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Section: Fungal Evsmentioning

confidence: 99%

Where Do They Come from and Where Do They Go: Candidates for Regulating Extracellular Vesicle Formation in Fungi

Oliveira

Rizzo

Joffe

et al. 2013

IJMS

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“…Mycelium was ground under liquid nitrogen, and the DNA extracted using the protocol described by Bogo et al (1996). Pelleted DNA was resuspended in 60 ll of ultrapure water and stored at 4°C.…”

Section: Dna Isolationmentioning

confidence: 99%

On the Diversity of the Laccase Gene: A Phylogenetic Perspective from Botryosphaeria rhodina (Ascomycota: Fungi) and Other Related Taxa

et al. 2009

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The present study is the first describing the sequencing of a fragment of the copper-oxidase domain of a laccase gene in the family Botryosphaeriaceae. The aim of this work was to assess the degree of genetic and evolutionary relationships of a laccase gene from Botryosphaeria rhodina MAMB-05 with other ascomycete and basidiomycete laccase genes. The 193-amino acid sequences of the copper-oxidase domain from several different fungi, insects, a plant, and a bacterial species were retrieved from GenBank and aligned. Phylogenetic analyses were performed using neighbor-joining, maximum parsimony, and Bayesian inference methods. The organisms studied clustered into five gene clades: fungi (ascomycetes and basidiomycetes), insects, plants, and bacteria. Also, the topologies showed that fungal laccases of the ascomycetes and basidiomycetes are clearly separated into two distinct clusters. This evidence indicated that B. rhodina MAMB-05 and other closely related ascomycetes are a new biological resource given the biotechnological potential of their laccase genes.

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“…No dsRNA elements were discovered in any of the Danish Metarhizium sp. or B. bassiana isolates, although their presence has been documented previously in populations of Metarhizium mostly from Brazil [32,33,34,35,36,37,38,39]. The number of isolates from other fungal species screened was very small and no dsRNA elements were found in any of these.…”

Section: Resultsmentioning

confidence: 79%

Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

Filippou

Garrido‐Jurado

Meyling

et al. 2018

Viruses

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The use of mycoviruses to manipulate the virulence of entomopathogenic fungi employed as biocontrol agents may lead to the development of novel methods to control attacks by insect pests. Such approaches are urgently required, as existing agrochemicals are being withdrawn from the market due to environmental and health concerns. The aim of this work is to investigate the presence and diversity of mycoviruses in large panels of entomopathogenic fungi, mostly from Spain and Denmark. In total, 151 isolates belonging to the genera Beauveria, Metarhizium, Lecanicillium, Purpureocillium, Isaria, and Paecilomyces were screened for the presence of dsRNA elements and 12 Spanish B. bassiana isolates were found to harbor mycoviruses. All identified mycoviruses belong to three previously characterised species, the officially recognised Beauveria bassiana victorivirus 1 (BbVV-1) and the proposed Beauveria bassiana partitivirus 2 (BbPV-2) and Beauveria bassiana polymycovirus 1 (BbPmV-1); individual B. bassiana isolates may harbor up to three of these mycoviruses. Notably, these mycovirus species are under distinct selection pressures, while recombination of viral genomes increases population diversity. Phylogenetic analysis of the RNA-dependent RNA polymerase gene sequences revealed that the current population structure in Spain is potentially a result of both vertical and horizontal mycovirus transmission. Finally, pathogenicity experiments using the Mediterranean fruit fly Ceratitis capitata showed no direct correlation between the presence of any particular mycovirus and the virulence of the B. bassiana isolates, but illustrated potentially interesting isolates that exhibit relatively high virulence, which will be used in more detailed virulence experimentation in the future.

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Double-stranded RNA and isometric virus-like particles in the entomopathogenic fungus Metarhizium anisopliae

Cited by 25 publications

References 13 publications

Where Do They Come from and Where Do They Go: Candidates for Regulating Extracellular Vesicle Formation in Fungi

Where Do They Come from and Where Do They Go: Candidates for Regulating Extracellular Vesicle Formation in Fungi

On the Diversity of the Laccase Gene: A Phylogenetic Perspective from Botryosphaeria rhodina (Ascomycota: Fungi) and Other Related Taxa

Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

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