Down-Regulation of ZmEXPB6 (Zea mays β-Expansin 6) Protein Is Correlated with Salt-mediated Growth Reduction in the Leaves of Z. mays L.

Geilfus, Christoph‐Martin; Ober, Dietrich; Eichacker, Lutz A.; Mühling, Karl H.; Zörb, Christian

doi:10.1074/jbc.m114.619718

Cited by 30 publications

(18 citation statements)

References 50 publications

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“…Changes in expansin expression facilitate this growth adaption in maize where water deficits enhance expression of selected expansin genes within the growth zone of roots [79]. In leaves a reverse response is observed: when maize seedlings were treated with 100 mM NaCl, leaf elongation was inhibited and ZmEXPB6 protein concomitantly vanished from leaf cell walls [80]. Leaf growth could be partially restored by exogenous application of recombinant ZmEXPB6 to leaf surfaces, an effect seen only in NaCl-treated plants, indicating a specific action of NaCl on ZmEXPB6 abundance.…”

Section: Stress Responses and Genetic Manipulation Of Expansin Gene Ementioning

confidence: 99%

Plant expansins: diversity and interactions with plant cell walls

Cosgrove

2015

Current Opinion in Plant Biology

405

370

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Expansins were discovered two decades ago as cell wall proteins that mediate acid-induced growth by catalyzing loosening of plant cell walls without lysis of wall polymers. In the interim our understanding of expansins has gotten more complex through bioinformatic analysis of expansin distribution and evolution, as well as through expression analysis, dissection of the upstream transcription factors regulating expression, and identification of additional classes of expansin by sequence and structural similarities. Molecular analyses of expansins from bacteria have identified residues essential for wall loosening activity and clarified the bifunctional nature of expansin binding to complex cell walls. Transgenic modulation of expansin expression modifies growth and stress physiology of plants, but not always in predictable and even understandable ways.

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Section: Stress Responses and Genetic Manipulation Of Expansin Gene Ementioning

confidence: 99%

Plant expansins: diversity and interactions with plant cell walls

Cosgrove

2015

Current Opinion in Plant Biology

405

370

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“…IbEXP1, IbEXP2, and IbEXPL1 genes are located in sweet-potato [33], AtEXLA2 in are found in Arabidopsis [34], and TaEXPB7-B is located in wheat [35], which responds to heat. Additionally, the AsEXP1 gene is present in turf grass [36], and TaEXPA2 [37] and ZmEXPB6 is located in maize [38], which respond to salt tolerance. In rice, OsEXPA3 is involved in root system development and is highly induced when responding to salt stress [39].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba

Kang

Paek

et al. 2020

IJMS

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Expansins are key regulators of cell-wall extension and are also involved in the abiotic stress response. In this study, we evaluated the function of OsEXPA7 involved in salt stress tolerance. Phenotypic analysis showed that OsEXPA7 overexpression remarkably enhanced tolerance to salt stress. OsEXPA7 was highly expressed in the shoot apical meristem, root, and the leaf sheath. Promoter activity of OsEXPA7:GUS was mainly observed in vascular tissues of roots and leaves. Morphological analysis revealed structural alterations in the root and leaf vasculature of OsEXPA7 overexpressing (OX) lines. OsEXPA7 overexpression resulted in decreased sodium ion (Na+) and accumulated potassium ion (K+) in the leaves and roots. Under salt stress, higher antioxidant activity was also observed in the OsEXPA7-OX lines, as indicated by lower reactive oxygen species (ROS) accumulation and increased antioxidant activity, when compared with the wild-type (WT) plants. In addition, transcriptional analysis using RNA-seq and RT-PCR revealed that genes involved in cation exchange, auxin signaling, cell-wall modification, and transcription were differentially expressed between the OX and WT lines. Notably, salt overly sensitive 1, which is a sodium transporter, was highly upregulated in the OX lines. These results suggest that OsEXPA7 plays an important role in increasing salt stress tolerance by coordinating sodium transport, ROS scavenging, and cell-wall loosening.

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“…Na al. (55). In brief, frozen-thawed leaf segments (2.5 cm length * 0.75 cm width) were connected to the LVDT by a thread (via glue) that was pulled over the wheel of the potentiometer.…”

Section: Ion Analysismentioning

confidence: 99%

Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves

Geilfus

Tenhaken

Carpentier

2017

Journal of Biological Chemistry

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During chloride salinity, the pH of the leaf apoplast (pHapo) transiently alkalizes. There is an ongoing debate about the physiological relevance of these stress-induced pHapo changes. Using proteomic analyses of expanding leaves of corn (Zea mays L.), we show that this transition in pHapo conveys functionality by (i) adjusting protein abundances and (ii) affecting the rheological properties of the cell wall. pHapo was monitored in planta via microscopy-based ratio imaging, and the leaf-proteomic response to the transient leaf apoplastic alkalinization was analyzed via ultra-high-performanceliquid-chromatography-MS. This analysis identified 1459 proteins, of which 44 exhibited increased abundance specifically through the chloride-induced transient rise in pHapo. These elevated protein abundances did not directly arise from high tissue concentrations of Cl -or Na + but were due to changes in the pHapo. Most of these proteins functioned in growth-relevant processes and in the synthesis of cell wallbuilding components such as arabinose. Measurements with a linear-variable differential transducer revealed that the transient alkalinization rigidified (i.e. stiffened) the cell wall during the onset of chloride salinity. A decrease in t-coumaric-and t-ferulicacid indicates that the wall stiffening arises from cross-linkage to cell wall polymers. We conclude that the pH of the apoplast represents a dynamic factor that is mechanistically coupled to cellular responses to chloride stress. By hardening the wall, the increased pH abrogates wall loosening required for cell expansion and growth. We conclude that the transient alkalinization of the leaf apoplast is related to salinity-induced growth reduction.

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Down-Regulation of ZmEXPB6 (Zea mays β-Expansin 6) Protein Is Correlated with Salt-mediated Growth Reduction in the Leaves of Z. mays L.

Cited by 30 publications

References 50 publications

Plant expansins: diversity and interactions with plant cell walls

Plant expansins: diversity and interactions with plant cell walls

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves

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