Downregulation of MicroRNA‐320d predicts poor overall survival and promotes the growth and invasive abilities in glioma

Qin, Chong-Zhen; Lv, Qiao‐Li; Yang, Yantao; Zhang, Jingmin; Zhang, Xiaojian; Zhou, Hong‐Hao

doi:10.1111/cbdd.12906

Cited by 25 publications

(19 citation statements)

References 37 publications

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“…Although recent studies have reported the functions of miR-320d in several cancer types, such as colorectal cancer [15], renal cell carcinoma [16], glioma [23], et al, less studies are focusing on the potential targeted genes of miR-320d. As the classical function of microRNA is silencing targeted genes by completely or incompletely pairing to the 3′-UTR of the specific genes, we screened the miR-320d targeted genes through the TargetScan website and other microRNA databases, and eventually found that FoxM1 contains two mRNA 3′-UTR binding sites for miR-320d.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

et al. 2020

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Recent evidences demonstrate that dysregulated expression of microRNA-320d (miR-320d) has been associated with several cancer development and progression. However the effect of miR-320d on gastric cardiac adenocarcinoma (GCA) and the association of miR-320d with its potential gene target FoxM1 remain unclear. Here, we evaluated expression profile of miR-320d and FoxM1 in 60 human GCA tissues and GCA cell lines (OE-19 and SK-GT2). Immunohistochemistry, qualitative PCR and western-blotting were performed in GCA tissues to detect the expression level of miR-320d and FoxM1. CCK-8, transwell, wound-healing assays, and in vivo experiments were conducted using GCA cells that treated with miR-320d mimics or inhibitors to evaluate the biological functions of miR-320d. Luciferase reporter assay was conducted to confirm possible binding sites of FoxM1 for miR-320d. Compared with paired noncancerous tissues, it showed that miR-320d expression was significantly decreased in GCA specimens (P < 0.0001), while FoxM1 was significantly upregulated in GCA tissues (P < 0.0001). Modulating miR-320d function by transfection of miR-320 mimics or inhibitor led to inhibition or promotion of GCA cell proliferation and invasion, thus regulating tumor progression in GCA-tumor bearing mice. The mechanism analysis of miR-320d/FoxM1 showed that FoxM1 has two miR-320d binding sites in its 3′-untranslated region (3′-UTR), that contributes to regulation of the cell biological behaviors. Taken together, our data suggested that miR-320d acts as a tumor suppressor in GCA by directly targeting FoxM1 and thus potentially serves as a biomarker for anti-GCA therapy in GCA patients.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

et al. 2020

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“…Previous studies have reported that miR-1290 and miR-320d were significantly dysregulated in various cancers, including CRC10-14. Mao et al declared that miR-1290 functions as a tumor oncogene to promote cancer progression by targeting nuclear factor I/X (NFIX) in esophageal squamous cell carcinoma (ESCC)15; Ma et al reported that miR-1290 expression increases in CRC tissues and could contribute to cancer cell proliferation by targeting inositol Polyphosphate 4-Phosphatase B (INPP4B) 16; Tadano et al demonstrated miR-320 family, including miR-320a, miR-320b, miR-320c, miR-320d, and miR-320e, were frequently downregulated in colorectal adenoma and submucosal invasive carcinoma tissues and regulated tumor proliferation by targeting CDK6 17; In addition, Qin et al revealed that the expression of miR-320d is significantly decreased in glioma tissues and cell lines and overexpression of miR-320d could suppress cell growth, migration and invasion, and induce cell apoptosis as well as cell cycle at G0/G1 arrest18. Similarly, our results exhibited that circulating miR-1290 was significantly associated with tumor size, TNM stage, lymph node metastasis, and distant metastasis, and circulating miR-320d was significantly correlated with tumor size, advanced TNM stage, lymph node metastasis, and distant metastasis.…”

Section: Discussionmentioning

confidence: 99%

Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer

Liu¹,

Xu²,

Pan³

et al. 2019

J. Cancer

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Background: The lack of screening methods with high diagnostic utility leads to colorectal cancer (CRC) patients usually diagnosed in advanced stages which results in high mortality. This study aimed to identify novel circulating miRNAs as biomarkers for the early detection of CRC.Materials and Methods: Total 205 participants were enrolled in this study. First, two dysregulated candidate miRNAs were selected after integrated analysis of four GEO datasets. Then, the expression of these two miRNAs in plasma samples were tested through qRT-PCR. Training phase and validation phase were designed to verify the diagnostic value of these two miRNAs using receiver operating characteristic curve (ROC) analysis.Results: After integrated analysis of GEO datasets, we discovered miR-1290 and miR-320d were dysregulated in colorectal adenoma and adenocarcinoma tissues, and circulating miR-1290 and miR-320d in CRC patients were tumor-derived. Thereafter, circulating miR-1290 and miR-320d were selected to further investigate their potential for early diagnosis of CRC. Plasma miR-1290 expression could differentiate adenoma and CRC patients from healthy controls with area under the curve (AUC) of 0.78 and 0.88. Similarly, plasma miR-320d expression could discriminate adenoma and CRC patients from healthy controls with AUC of 0.74 and 0.81.Conclusions: Circulating miR-1290 and miR-320d are novel promising biomarkers for early diagnosis of CRC.

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“…12 However, the expression pattern and biological function of miR-320d in breast cancer are largely unknown.…”

Section: -10mentioning

confidence: 99%

“…Previous studies have reported the function of miR-320d in glioma, colorectal adenoma and B-cell lymphoma. 11,12,30 In the present study, we rst investigated the clinical signicance of miR-320d and explored its role in cell growth, apoptosis, migration and invasion of breast cancer. The results demonstrated that the expression of miR-320d in breast cancer tissues was signi-cantly lower than that in normal tissues.…”

Section: 29mentioning

confidence: 99%

MiR-320d suppresses the progression of breast cancervialncRNA HNF1A-AS1 regulation and SOX4 inhibition

Shi

et al. 2018

RSC Adv.

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MicroRNA-320d (miR-320d) is a novel cancer-related miRNA and functions as a tumor suppressor in human cancers. However, the expression pattern and function of miR-320d in breast cancer remain largely unknown. In the present study, we found that the expression level of miR-320d in breast cancer tissues and cells was significantly lower than in non-tumor tissues and MCF-10A cells. Decreased miR320d was associated with poor overall survival in patients with breast cancer. Overexpression of miR320d inhibited proliferation, migration, and invasion and promoted apoptosis of breast cancer cells. In addition, the long non-coding RNA, HNF1A antisense RNA 1 (HNF1A-AS1) was up-regulated in both breast cancer tissues and cell lines. HNF1A-AS1 suppressed the expression and function of miR-320d.Moreover, SRY-related HMG-box 4 (SOX4) was speculated and confirmed as a target of miR-320d. We also demonstrated that HNF1A-AS1 may function as a sponge competitive endogenous RNA for miR320d, and thus regulate the expression of SOX4. Taken together, our study has identified a novel signaling pathway through which miR-320d exerts its anti-carcinogenic roles and suggested that the HNF1A-AS1/miR-320d/SOX4 may be a potential target for the therapy of breast cancer.

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Downregulation of MicroRNA‐320d predicts poor overall survival and promotes the growth and invasive abilities in glioma

Cited by 25 publications

References 37 publications

MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma

Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer

MiR-320d suppresses the progression of breast cancervialncRNA HNF1A-AS1 regulation and SOX4 inhibition

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